Pressure-induced expression changes in segmental flow regions of the human trabecular meshwork.
Janice A. Vranka,Ted S. Acott +1 more
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TL;DR: This work identified molecular expression differences between segmental flow regions of the TM from anterior segments perfused at either physiological or elevated pressure and found a limited subset of ECM genes is differentially regulated in both high and low flow regions and in response to elevated pressure.
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About: This article is published in Experimental Eye Research. The article was published on 01 May 2017. and is currently open access. The article focuses on the topics: Trabecular meshwork & Extracellular matrix.
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Normal and glaucomatous outflow regulation
TL;DR: A hypothetical model, based on considerable available data, posits that the continuous SCE basal lamina, which lies between 125 and 500 nm beneath the S CE basal surface, is the primary source of normal resistance and has the potential to advance the understanding of IOP regulation and of glaucoma.
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Matrix Metalloproteinases and Glaucoma Treatment.
TL;DR: It is hypothesized that the higher concentrations of bimatoprost achieved in ocular outflow tissues with the implant produce greater MMP upregulation and more extensive, sustained MMP-mediated target tissue remodeling, providing an extended duration of effect.
86
Biomechanical Rigidity and Quantitative Proteomics Analysis of Segmental Regions of the Trabecular Meshwork at Physiologic and Elevated Pressures
Janice A. Vranka,Julia Staverosky,Ashok P. Reddy,Phillip A. Wilmarth,Larry L. David,Ted S. Acott,Paul Russell,Vijay Krishna Raghunathan +7 more
TL;DR: Data show changes in biomechanical properties of segmental regions within the TM in response to elevated pressure, and levels of specific ECM proteins that are specifically involved in outflow resistance and IOP homeostasis.
69
Open-angle glaucoma: therapeutically targeting the extracellular matrix of the conventional outflow pathway.
TL;DR: The role of ECM of the TM in outflow homeostasis and its relevance as a target for glaucoma therapy is discussed, including progress in development of topical eye formulations, together with gene therapy approaches based on inducible, virally-mediated expression of matrix metalloproteinases to enhance aqueous outflow.
51
Characterization of Human Transition Zone Reveals a Putative Progenitor-Enriched Niche of Corneal Endothelium.
Gary Hin-Fai Yam,Xin-Yi Seah,Nur Zahirah Binte M Yusoff,Melina Setiawan,Stephen Wahlig,Hla Myint Htoon,Gary S L Peh,Gary S L Peh,Viridiana Kocaba,Jodhbir S. Mehta +9 more
TL;DR: A novel inner TZ containing progenitor-like cells is identified, which could serve the regenerative potential for corneal endothelium.
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References
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Two Pore Types in the Inner-Wall Endothelium of Schlemm's Canal
TL;DR: The dependence of intracellular pore density on postmortem time and on volume of fixative passed through the outflow pathway suggests that these pores are artifacts of tissue fixation or processing conditions, while that border pores do not depend on such conditions and that their presence is correlative with perfusion pressure suggests that this population may be nonartifactual.
•Journal Article
Novel Molecular Insights Into Rho GTPase-Induced Resistance to Aqueous Humor Outflow Through the Trabecular Meshwork
TL;DR: Collectively, these data demonstrate that sustained activation of Rho GTPase signaling in the aqueous humor outflow pathway increases resistance to aqueosin outflow through the trabecular pathway by influencing the actomyosin assembly, cell adhesive interactions, and the expression of ECM proteins and cytokines in TM cells.
99
Novel molecular insights into RhoA GTPase-induced resistance to aqueous humor outflow through the trabecular meshwork
TL;DR: The role of RhoA GTPase-mediated actomyosin organization, cell adhesive interactions, and gene expression in regulation of aqueous humor outflow was investigated using adenoviral vector-driven expression of constitutively active mutant of rhoA (RhoAV14) as mentioned in this paper.
98
Extracellular matrix gene alternative splicing by trabecular meshwork cells in response to mechanical stretching.
TL;DR: Alternative splicing events led to the modulation of potential GAG attachment sites and other ECM-binding motifs during the ECM remodeling that occurs coincident with homeostatic restoration of IOP to normal.
98
Effects of Modifiers of Glycosaminoglycan Biosynthesis on Outflow Facility in Perfusion Culture
TL;DR: Disrupting GAG chain biosynthesis increased outflow facility in perfusion culture and induced atypical ECM molecule interactions in cell culture, providing direct evidence of the critical role of GAG chains in regulating outflow resistance in human TM.