9 Papers
79 Citations
Siyi Hu is an academic researcher from University of Science and Technology of China. The author has contributed to research in topics: Affinity chromatography & Pichia pastoris. The author has an hindex of 6, co-authored 9 publications.
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Papers
Epitope mapping and structural analysis of an anti-ErbB2 antibody A21: Molecular basis for tumor inhibitory mechanism
Siyi Hu,Zhiqiang Zhu,Liangwei Li,Liang Chang,Wei-Fang Li,Liansheng Cheng,Maikun Teng,Jing Liu +7 more
TL;DR: The results showed that A21 recognized a conformational epitope comprising a large region mostly from ErbBB2 extracellular subdomain I with several surface‐exposed residues important for the binding affinity, which provided unique functional properties of A21 that are quite different from two broadly used anti‐ErbB2 mAbs, Herceptin and 2C4.
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Purification and characterization of human IL-10/Fc fusion protein expressed in Pichia pastoris.
TL;DR: The designed fusion protein containing human IL-10 and an IgG Fc fragment and expressed in Pichia pastoris displayed highly specific biological activity, which was slightly higher than that of the commercial recombinant human Il-10 (rhIL-10), and was useful in the large-scale production of hIL- 10/Fc fusion protein for both research and therapeutic applications.
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In vivo activity of novel anti-ErbB2 antibody chA21 alone and with Paclitaxel or Trastuzumab in breast and ovarian cancer xenograft models
Guodong Shen,Huang Hui,Anli Zhang,Ting Zhao,Siyi Hu,Liansheng Cheng,Jing Liu,Weihua Xiao,Bin Ling,Qiang Wu,Lihua Song,Wei Wei +11 more
TL;DR: The study reveals that the effects by chA21 to cause an enhanced inhibition on cancer cell proliferation and angiogenesis was highly associated with the intrinsic ability of chA 21 to down-regulate ErbB2 receptor, inhibit downstream MAPK and PI3K-AKT signal transduction and activate natural killer cells.
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Production and characterization of recombinant 9 and 15 kDa granulysin by fed-batch fermentation in Pichia pastoris.
Yugang Guo,Gan Luan,Guodong Shen,Lidan Wu,Jia Hao,Yongjun Zhong,Rui Li,Guangwei Li,Yi Shen,Jie Sun,Siyi Hu,Weihua Xiao +11 more
TL;DR: Results suggest that fermentation in P. pastoris provides a sound strategy for large-scale recombinant granulysin production that may be used in clinical applications and basic research.
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Purification and characterization of a single-chain chimeric anti-p185 antibody expressed by CHO–GS system
TL;DR: The high purity of the recombinant antibody allowed us to identify a number of its intrinsic molecular properties, including antigen binding activity, measurement of affinity constant, N-terminal sequencing, and mass spectrometer analysis.
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