9 Papers
28 Citations
Jia Hao is an academic researcher from University of Science and Technology of China. The author has contributed to research in topics: Pichia pastoris & Monoclonal antibody. The author has an hindex of 4, co-authored 9 publications.
Chat about Author
Papers
The expression and characterization of functionally active soluble CD83 by Pichia pastoris using high-density fermentation.
Yugang Guo,Rui Li,Xiaoping Song,Yongjun Zhong,Chenguang Wang,Jia Hao,Lidan Wu,Dong Wang,Fang Fang,Jiajia Ma,Wenyao Kang,Jie Sun,Zhigang Tian,Weihua Xiao +13 more
TL;DR: In vitro tests indicated that recombinant sCD83 bound to its putative counterpart on monocytes and specifically blocked the binding of anti-CD83 antibodies to cell surface CD83 on DCs, which suggests that s CD83 acts as a potential immune suppressor.
Balancing the Expression and Production of a Heterodimeric Protein: Recombinant Agkisacutacin as a Novel Antithrombotic Drug Candidate
Yugang Guo,Jing Wu,Jia Hao,Wei Chen,Shao Changsheng,Lei Zhao,Jiajia Ma,Rui Li,Yongjun Zhong,Fang Fang,Dong Wang,Jie Sun,Fang Qian,Xiangrong Dai,Guohui Zhang,Zhigang Tian,Benjamin Xiaoyi Li,Weihua Xiao +17 more
TL;DR: The recombinant Agkisacucetin possessed extremely similar binding affinity to recombinant GPIb and human platelets in in vitro assays, and its ristocetin-induced platelet aggregation activity ex vivo was identical to that of the extracted native Agkistrodon acutus, demonstrating that the yeast-derived Agk Isaccuetin could be an effective alternative to native AgKisacUCetin.
Production and characterization of recombinant 9 and 15 kDa granulysin by fed-batch fermentation in Pichia pastoris.
Yugang Guo,Gan Luan,Guodong Shen,Lidan Wu,Jia Hao,Yongjun Zhong,Rui Li,Guangwei Li,Yi Shen,Jie Sun,Siyi Hu,Weihua Xiao +11 more
TL;DR: Results suggest that fermentation in P. pastoris provides a sound strategy for large-scale recombinant granulysin production that may be used in clinical applications and basic research.
11
Patent
Expression and preparation method of soluble CD83
Weihua Xiao,Yugang Guo,Rui Li,Yongjun Zhong,Jia Hao,Sun Hao,Zhigang Tian +6 more
- 14 Aug 2013
TL;DR: In this paper, an expression and preparation method of soluble CD83 (soluble CD83, sCD83) was provided, and the expression quantity was as high as 200mg/L, and through two-step purification, the purity was higher than 95%.
3
Patent
Anti-human CD83 monoclonal antibody as well as preparation, identification and application thereof
Xiao Weihua,Yugang Guo,Wu Jing,Rui Li,Jia Hao,Shao Changsheng,Wei Chen +6 more
- 13 May 2015
TL;DR: In this paper, an anti-human CD83 monoclonal antibody with the specificity direct at the amino acid sequences of 20 th and 145 th sites at the outer segment of a human CD83 cell was revealed.