Hao Chen
Chinese Academy of Sciences
7 Papers
1 Citations
Hao Chen is an academic researcher from Chinese Academy of Sciences. The author has contributed to research in topics: Metabolic engineering & Escherichia coli. The author has an hindex of 4, co-authored 7 publications.
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Papers
Computational redesign of enzymes for regio- and enantioselective hydroamination.
Ruifeng Li,Hein J. Wijma,Lu Song,Yinglu Cui,Marleen Otzen,Yu’e Tian,Jiawei Du,Tao Li,Dingding Niu,Yanchun Chen,Jing Feng,Jian Han,Hao Chen,Yong Tao,Dick B. Janssen,Bian Wu +15 more
TL;DR: Computational protein design, without subsequent directed evolution, rapidly provides a set of aspartase variants capable of biocatalytic asymmetric addition of ammonia to substituted acrylates, producing various enantiopure β-amino acids.
Metabolic engineering for efficient supply of acetyl-CoA from different carbon sources in Escherichia coli
TL;DR: The metabolically engineered E. coli strains for efficiently supplying acetyl-CoA from glucose, acetate, and fatty acid were constructed and applied in one-step biosynthesis of N-acetylglutamate (NAG) from glutamate and acetyl -CoA.
Metabolic engineering of Escherichia coli for production of L-aspartate and its derivative β-alanine with high stoichiometric yield.
TL;DR: This study designed a shortcut pathway of L-aspartate biosynthesis in Escherichia coli, with a maximized stoichiometric yield of 2 mol/mol glucose and the highest yield of β-alanine reported so far.
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Efficient production of myo-inositol in Escherichia coli through metabolic engineering.
TL;DR: An inositol-synthesis pathway from glucose with a theoretical stoichiometric yield of 1 mol inositl/mol glucose is designed and successfully biosynthesized after introducing two crucial enzymes: inositoli-3-phosphate synthase (IPS) from Trypanosoma brucei and inositols monophosphatase (IMP) from E. coli.
Menaquinone-7 production in engineered Escherichia coli
TL;DR: This report is the first to describe the metabolic engineering of MK-7 in E. coli, which provides a new perspective forMK-7 production and revealed a new bottleneck in the MK- 7 pathway at ubiE, suggesting an avenue for further optimization.
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