Optimizing human synovial fluid preparation for two-dimensional gel electrophoresis.
Carl Pc Chen,Chih-Chin Hsu,Wen-Lin Yeh,Hsiu-Chu Lin,Sen-Yung Hsieh,Shih-Cherng Lin,Tai-Tzung Chen,Max Jl Chen,Simon F. T. Tang +8 more
TL;DR: The acetone precipitation method and the combined treatment effect of acetone and2-DE Clean-Up Kit are not preferred in preparing SF samples for 2-DE analysis as both protein intensities and numbers decrease significantly.
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Abstract: Background
Proteome analysis is frequently applied in identifying the proteins or biomarkers in knee synovial fluids (SF) that are associated with osteoarthritis and other arthritic disorders. The 2-dimensional gel electrophoresis (2-DE) is the technique of choice in these studies. Disease biomarkers usually appear in low concentrations and may be masked by high abundant proteins. Therefore, the main aim of this study was to find the most suitable sample preparation method that can optimize the expression of proteins on 2-DE gels that can be used to develop a reference proteome picture for non-osteoarthritic knee synovial fluid samples. Proteome pictures obtained from osteoarthritic knee synovial fluids can then be compared with the reference proteome pictures obtained in this study to assist us in identifying the disease biomarkers more correctly.
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Citations
A normative study of the synovial fluid proteome from healthy porcine knee joints.
Tue Bjerg Bennike,Ugur M. Ayturk,Carla M. Haslauer,John W. Froehlich,Benedikt L. Proffen,Omar S. Barnaby,Svend Birkelund,Martha M. Murray,Matthew L. Warman,Allan Stensballe,Hanno Steen +10 more
TL;DR: Knowing the synovial fluid proteome of a healthy joint will help to identify mechanisms that cause joint disease and pathways involved in disease progression, and underlining the detected plasma derived nature of many synvial fluid components.
Human body-fluid proteome: quantitative profiling and computational prediction
TL;DR: The experimental progresses using a combination of conventional and high-throughput technologies, along with the major discoveries, are summarized, and the emerging computational work on protein prediction based on support vector machine, ranking algorithm, and protein–protein interaction network is surveyed.
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Delineating the synovial fluid proteome: recent advancements and ongoing challenges in biomarker research.
TL;DR: Some of the potential pitfalls of biomarker studies using SF are focused on, the status of the field of SF proteomics in general is summarized, and some of the most promising biomarker study approaches using proteomics are discussed.
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Shell proteome of rhynchonelliform brachiopods
TL;DR: This study employed a proteomic approach to identify numerous peptides that constitute the shell proteins, in three rhynchonellid brachiopods from different localities, suggesting that the shell protein repertoires identified thus far, differ from that of better known calcifying metazoans, such as molluscs.
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Subchondral bone proteomics in osteoarthritis: Current status and perspectives
TL;DR: Osteoarthritis was conceived as a “wear and tear” problem of articular cartilage, yet there is a lack of treatment options to delay or rescue articularcartilage degeneration once it is established.
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