Early-replicating heterochromatin
TL;DR: It is found that heterochromatic centromeres and silent mating-type cassettes replicate in early S phase, and late replication is not an obligatory feature ofheterochromatin.
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Abstract: Euchromatin, which has an open structure and is frequently transcribed, tends to replicate in early S phase. Heterochromatin, which is more condensed and rarely transcribed, usually replicates in late S phase. Here, we report significant deviation from this correlation in the fission yeast, Schizosaccharomyces pombe. We found that heterochromatic centromeres and silent mating-type cassettes replicate in early S phase. Only heterochromatic telomeres replicate in late S phase. Research in other laboratories has shown that occasionally other organisms also replicate some of their heterochromatin in early S phase. Thus, late replication is not an obligatory feature of heterochromatin.
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Citations
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Tom Volpe,Catherine A. Kidner,Ira M. Hall,Ira M. Hall,Grace Teng,Grace Teng,Shiv I. S. Grewal,Robert A. Martienssen +7 more
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TL;DR: In this article, two-dimensional agarose gel electrophoresis was used to identify the origin of the yeast autonomous replication sequence ARS1 in the Saccharomyces cerevisiae.
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Ira M. Hall,Gurumurthy D. Shankaranarayana,Ken-ichi Noma,Nabieh Ayoub,Amikam Cohen,Shiv I. S. Grewal,Shiv I. S. Grewal +6 more
TL;DR: It is demonstrated that a centromere-homologous repeat present at the silent mating-type region is sufficient for heterochromatin formation at an ectopic site, and that its repressive capacity is mediated by components of the RNA interference (RNAi) machinery.
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Transitions in Distinct Histone H3 Methylation Patterns at the Heterochromatin Domain Boundaries
TL;DR: It is shown that distinct site-specific histone H3 methylation patterns define euchromatic and heterochromatic chromosomal domains within a 47-kilobase region of the mating-type locus in fission yeast.
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Cell cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast.
TL;DR: Fluorescence in situ hybridization (FISH) shows that fission yeast centromeres and telomeres make up specific spatial arrangements in the nucleus and is a powerful tool for analyzing mitotic chromosome movement and disjunction using various mutants.
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