Changes in synaptic potential properties during acetylcholine receptor accumulation and neurospecific interactions in Xenopus nerve-muscle cell culture.

TL;DR: It is shown that spontaneous and nerve-evoked synaptic currents during the initial period of nerve-muscle contact in Xenopus cell cultures are similar to those observed at naturally occurring synapses and the transition from low- to high-efficacy transmission during the early phase of contact may reflect the process of selective adhesion between the cells, and thus signify the formation of specific synapse.

TL;DR: It is hypothesized that the interaction of LN with α-DG contributes to the growth and/or stabilization of AChR microaggregates into macroaggregate at the developing NMJ via a MuSK-independent mechanism.

TL;DR: This study of the identified functional contacts may help to understand the structural basis for early nerve-muscle interaction and the functional significance of various synaptic specializations.

TL;DR: It is found that neurons which had previously made synapse with other muscle cells in the culture all failed to release ACh from the soma and showed reduced release capability at the neurite for the first 30 min to 1 hr of contact with a myoball, suggesting that, during synapatogenesis, there is a depletion of ACh molecules and/or substances responsible for the triggering of their release in the extrasynaptic regions of the neuron.

TL;DR: It is suggested that post‐synaptic potentiation between quanta may play a role in signalling at synapses in which non‐linear dose‐response characteristics have been observed and where transmitter is not as repidly inactivated as the neuromuscular synapse.

TL;DR: The most slowly eluting peak, Peak IV, has the highest quantum efficiency and binds irreversibly to acetylcholine receptors on electroplax fragments and labels the fragments more intensely than Peaks I–III, which are identified as mixtures of multiply labeled tetramethyl rhodamine α-bungarotoxin.

TL;DR: Clonal neuroblastoma x glioma hybrid cells frequently formed synapses with clonal mouse striated muscle cells and acetylcholine sensitivity measurements indicate that acetylCholine receptors of clonal myotubes are distributed more uniformly over the cell surface than the receptors of cultured mouse embryo myot tubes.

TL;DR: Reinnervation or cross‐reinnervation of the sartorius and the iliofibularis muscles in adults or at a late stage of development simply reconstituted the normal focal and distributed innervation patterns of the muscles, as found in the control muscles of the contralateral and unoperated legs.