Fred J. Sigworth
Yale University
163 Papers
2.1K Citations
Fred J. Sigworth is an academic researcher from Yale University. The author has contributed to research in topics: Gating & Chemistry. The author has an hindex of 63, co-authored 155 publications. Previous affiliations of Fred J. Sigworth include Howard Hughes Medical Institute & Max Planck Society.
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Papers
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
TL;DR: The extracellular patch clamp method, which first allowed the detection of single channel currents in biological membranes, has been further refined to enable higher current resolution, direct membrane patch potential control, and physical isolation of membrane patches.
Quantifying the local resolution of cryo-EM density maps.
TL;DR: By evaluating the local resolution of single-particle reconstructions and subtomogram averages for four example data sets, this work reports variable resolution across a 4- to 40-Å range.
Importance of the Debye screening length on nanowire field effect transistor sensors.
TL;DR: The appropriate conditions under which the selective binding of macromolecules is accurately sensed with NW-FET sensors are shown.
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Primary structure and functional expression of a mammalian skeletal muscle sodium channel
James S. Trimmer,Sharon S. Cooperman,Sally A. Tomiko,Jiuying Zhou,Shelia M. Crean,Mary B. Boyle,Roland G. Kalen,Zu-Hang Sheng,Robert L. Barchi,Fred J. Sigworth,Richard H. Goodman,William S. Agnew,Gail Mandel +12 more
TL;DR: The isolation and characterization of a cDNA encoding the alpha subunit of a new voltage-sensitive sodium channel, microI, from rat skeletal muscle is described, and northern blot analysis indicates that the 8.5 kb microI transcript is preferentially expressed in skeletal muscle.
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Single Na + channel currents observed in cultured rat muscle cells
Fred J. Sigworth,Erwin Neher +1 more
TL;DR: The first observations of currents through individual Na+ channels under physiological conditions using an improved version of the extracellular patch-clamp technique on cultured rat muscle cells support earlier inferences about channel gating and show a single-channel conductance of approximately 18 pS.
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