Diane D. Chen
Boston Children's Hospital
5 Papers
6 Citations
Diane D. Chen is an academic researcher from Boston Children's Hospital. The author has contributed to research in topics: Enhancer & Cas9. The author has an hindex of 5, co-authored 5 publications. Previous affiliations of Diane D. Chen include Harvard University.
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Papers
BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis
Matthew C. Canver,Elenoe C. Smith,Falak Sher,Luca Pinello,Neville E. Sanjana,Ophir Shalem,Diane D. Chen,Patrick G. Schupp,Divya S. Vinjamur,Sara P. Garcia,Sidinh Luc,Ryo Kurita,Yukio Nakamura,Yuko Fujiwara,Yuko Fujiwara,Takahiro Maeda,Guo-Cheng Yuan,Feng Zhang,Stuart H. Orkin,Stuart H. Orkin,Daniel E. Bauer +20 more
TL;DR: A pooled clustered regularly interspaced palindromic repeat-Cas9 guide RNA libraries are developed to perform in situ saturating mutagenesis of the human and mouse enhancers and reveal critical minimal features and discrete vulnerabilities of these enhancers.
Genome-wide association study of red blood cell traits in Hispanics/Latinos: The Hispanic Community Health Study/Study of Latinos
Chani J. Hodonsky,Deepti Jain,Ursula M. Schick,Ursula M. Schick,Jean Morrison,Lisa M. Brown,Caitlin P. McHugh,Claudia Schurmann,Diane D. Chen,Yongmei Liu,Paul L. Auer,Cecilia A. Laurie,Kent D. Taylor,Brian L. Browning,Yun Li,George Papanicolaou,Jerome I. Rotter,Ryo Kurita,Yukio Nakamura,Sharon R. Browning,Ruth J. F. Loos,Kari E. North,Cathy C. Laurie,Timothy A. Thornton,Nathan Pankratz,Daniel E. Bauer,Daniel E. Bauer,Tamar Sofer,Alex P. Reiner +28 more
TL;DR: It is demonstrated that disruption of the regulatory element harboring rs3812049 affects transcription of SLC12A2 and LINC01184 in human erythroid progenitor cells, reinforcing the importance of genetic study of diverse ancestral populations, in particular Hispanics/Latinos.
Crispr-Cas9 Saturating Mutagenesis Reveals an Achilles Heel in the BCL11A Erythroid Enhancer for Fetal Hemoglobin Induction (by Genome Editing)
Daniel E. Bauer,Matthew C. Canver,Elenoe C. Smith,Falak Sher,Luca Pinello,Neville E. Sanjana,Ophir Shalem,Diane D. Chen,Patrick G. Schupp,Divya S. Vinjamur,Sara P. Garcia,Sidinh Luc,Ryo Kurita,Yukio Nakamura,Yuko Fujiwara,Takahiro Maeda,Guo-Cheng Yuan,Guillaume Lettre,Feng Zhang,Stuart H. Orkin +19 more
TL;DR: It is shown that in primary CD34+ hematopoietic stem and progenitor derived human erythroid precursors that delivery of an individual gRNA and Cas9 is sufficient to produce robust reinduction of HbF.
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BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis
Matthew C. Canver,Elenoe C. Smith,Falak Sher,Luca Pinello,Neville E. Sanjana,Ophir Shalem,Diane D. Chen,Patrick G. Schupp,Divya S. Vinjamur,Sara P. Garcia,Sidinh Luc,Ryo Kurita,Yukio Nakamura,Yuko Fujiwara,Yuko Fujiwara,Takahiro Maeda,Guo-Cheng Yuan,Feng Zhang,Stuart H. Orkin,Stuart H. Orkin,Daniel E. Bauer +20 more
- 01 Sep 2015
TL;DR: The authors developed pooled clustered regularly interspaced palindromic repeat (CRISPR)-Cas9 guide RNA libraries to perform in situ saturating mutagenesis of the human and mouse enhancers.
Quantitative assessment of timing, efficiency, specificity and genetic mosaicism of CRISPR/Cas9-mediated gene editing of hemoglobin beta gene in rhesus monkey embryos.
Uros Midic,Pei Hsuan Hung,Kailey A. Vincent,Benjamin Goheen,Patrick G. Schupp,Diane D. Chen,Daniel E. Bauer,Daniel E. Bauer,Catherine A. VandeVoort,Keith E. Latham +9 more
TL;DR: The data demonstrate an ability to achieve a high efficiency of gene editing in rhesus monkey zygotes, with no detected off-target effects at selected off- target loci.