Topic
Hyphal tip
About: Hyphal tip is a research topic. Over the lifetime, 664 publications have been published within this topic receiving 25246 citations.
Papers published on a yearly basis
1 / 2
Papers
1 Jan 2004
TL;DR: Three new species of Trichoderma atroviride, known as one of the most important biocontrol agents against plant diseases caused by some pathogenic fungi, are reported from different parts of Iran.
Abstract: Some 350 Trichoderma isolates were obtained from soil samples collected from different parts of Iran. Cultures were purified on 2% water agar by hyphal tip method prior to identification. Obtained isolates were identified using morphological features including colony characters (pigmentation and growth rate on PDA) and microscopic characters such as shape of conidiophores, shape and size of conidia and phialides. Microscopic features of conidiophores and shape and size of conidia were studied and recorded 3–5 days after inoculation on cultures grown on 2% MEA at 20°C under ambient laboratory conditions by using light microscopy and scanning electron microscopy (SEM). Ten species including T. citrinoviride, T. longibrachiatum, T. saturnisporum, T. hamatum, T. harzianum, T. inhamalum, T. tomentosum, T. virens, T. asperellum and T. koningii were already identified and reported from Iran (ZAFARI et al. 2002) and three new species, T. ghanense (Fig. 1), T. spirale (Fig. 2) and T. atroviride (Fig. 3) from sections Longibrachiatum, Pachybasium and Trichoderma, respectively, are reported here. Trichoderma atroviride is known as one of the most important biocontrol agents against plant diseases caused by some pathogenic fungi.
3 citations
TL;DR: A novel, but hitherto unknown mechanism for reconciling two fundamental processes that utilize the same macromolecular template that allows for rapid growth without compromising chromosome replication in filamentous bacteria and may have implications for evolution of filamentous growth in micro-organisms, where uncoupling of DNA replication from cell division is required.
Abstract: Reconciling transcription and DNA replication in the growing hyphae of the filamentous bacterium Streptomyces presents several physical constraints on growth due to their apically extending and branching, multigenomic cells and chromosome replication being independent of cell division. Using a GFP translational fusion to the β'-subunit of RNA polymerase (rpoC-egfp), in its native chromosomal location, we observed growing Streptomyces hyphae using time-lapse microscopy throughout the lifecycle and under different growth conditions. The RpoC-eGFP fusion co-localized with DNA around 1.8 µm behind the extending tip, whereas replisomes localize around 4-5 µm behind the tip, indicating that at the growing tip, transcription and chromosome replication are to some degree spatially separated. Dual-labelled RpoC-egfp/DnaN-mCherry strains also indicate that there is limited co-localization of transcription and chromosome replication at the extending hyphal tip. This likely facilitates the use of the same DNA molecule for active transcription and chromosome replication in growing cells, independent of cell division. This represents a novel, but hitherto unknown mechanism for reconciling two fundamental processes that utilize the same macromolecular template that allows for rapid growth without compromising chromosome replication in filamentous bacteria and may have implications for evolution of filamentous growth in micro-organisms, where uncoupling of DNA replication from cell division is required.
3 citations
TL;DR: In this article, the function of AAA ATPase AipA and AoAbp1 in endocytosis using enhanced green fluorescent protein (EGFP)-tagged arginine permease AoCan1 as an endocytic marker.
3 citations
TL;DR: A set of five carbohydrate-specific monoclonal antibodies were used to probe the ultrastructure of the walls of the soybean pathogen Phytophthora megasperma f.sp.
3 citations
TL;DR: The results show that the effect of an antifungal agent on a hypha depends on the region of the hypha exposed, and cytoplasmic streaming may play key role in the transmission of antIFungal effects within a single hypha.
Abstract: A micro-compartment culture method was devised in which a single hypha of Rhizopus stolonifer growing on an agar section traversed an antifungal non-diffusible barrier to another agar section; thus the local environment of the distal or proximal part of the hypha could be controlled independently. The responses in terms of hyphal extension of the test fungus to local application of amphotericin B in this culture system were estimated by using an automatic analysing system. After hyphae had traversed the barrier, distal application of amphotericin B caused no appreciable effect on the proximal hyphae. In contrast, proximal application of amphotericin B caused inhibition of the extension of distal hyphae. The reversal of polarized cytoplasmic streaming also occured during the inhibition of distal hyphal extension. The extents of inhibition of the distal hyphal extension and the cytoplasmic streaming were dependent upon the hyphal distance between the amphotericin B application site and the hyphal tip. These results show that the effect of an antifungal agent on a hypha depends on the region of the hypha exposed. Cytoplasmic streaming may play key role in the transmission of antifungal effects within a single hypha.
3 citations
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Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 25 |
| 2020 | 18 |
| 2019 | 19 |
| 2018 | 15 |
| 2017 | 19 |
| 2016 | 17 |