Topic
Gene Expression Process
About: Gene Expression Process is a research topic. Over the lifetime, 41 publications have been published within this topic receiving 1210 citations.
Papers
TL;DR: In this article, the role of stochasticity qualitatively in a network of two genes was investigated and the authors found that the noise in gene expression process reduces the energy cost of protein synthesis.
Abstract: Gene expression and its regulation is a nonequilibrium stochastic process. Different molecules are involved in several biochemical steps in this process with low copies. It is observed that the stochasticity in biochemical processes is mainly due to the low copy number of the molecules present in the system. Several studies also show that the nonequilibrium biochemical processes require energy cost. But cellular system has developed itself through natural evolution by minimizing energy cost for optimum output. Here we study the role of stochasticity qualitatively in a network of two genes using stochastic simulation method and approximately measure the energy consumption for the gene expression process. We find that the noise in gene expression process reduces the energy cost of protein synthesis. Therefore, we argued that the stochasticity in gene expression may be a choice of cellular system for protein synthesis with minimum energy cost.
4 citations
TL;DR: A highly efficient transient gene expression system was established by optimizing significant factors; namely, the host cells, lipid-based transfection reagent, enhancer, and expression vector, and the novel combination method was successfully applied to human IgG and Fc protein production using Expi293 F cells.
4 citations
Patent•
11 Mar 2015
TL;DR: In this paper, a method for simultaneously cloning multiple exogenous genes to a microbial genome was proposed, which can organically integrate the whole gene expression process such as expression design, clone design, host cell modification, and the like.
Abstract: The invention discloses a method for simultaneously cloning multiple exogenous genes to a microbial genome. The method comprises steps of introducing a plurality of exogenous genes to a plurality of bidirectional gene expression carriers, and then simultaneously introducing the plurality of bidirectional gene expression carriers into a host microbe. Each bidirectional gene expression carrier comprises a bidirectional terminator and a bidirectional promoter. Except for the first bidirectional gene expression carrier and the last bidirectional gene expression carrier, the 3' ends of the bidirectional terminators of other bidirectional gene expression carriers and the 5' ends of the bidirectional terminators of the next bidirectional gene expression carriers have the same homologous arm. The 5' end of the bidirectional terminator of the first bidirectional gene expression carrier and the 3' end of the bidirectional terminator of the last bidirectional gene expression carrier can both carry out homologous recombination with the genomes of a host microbe. Through the provided method, established is a novel technology that can organically integrate the whole gene expression process such as expression design, clone design, host cell modification, and the like.
3 citations
Patent•
11 Dec 2002
TL;DR: In this article, a method of preparing human liver cell growth factor rhHGF in eukaryon expression system through cloning hHGF gene and constituting EK-expression plasmia pRC/CMV-rhHGF, CHO with stable cell strain and high expression efficiency and NRK with easy surviral, short liver period and fast growth speed are used as host cell to express rh HGF with wild activity.
Abstract: The present invention discloses a method of preparing human liver cell growth factor rhHGF in eukaryon expression system Through cloning hHGF gene and constituting eukaryon expression plasmia pRC/CMV-rhHGF, CHO with stable cell strain and high expression efficiency and NRK with easy surviral, short liver period and fast growth speed are used as host cell to express rhHGF with wild activity Compared with traditional prokaryon expression system, the eukaryon expression system of the present invention has the advantages of easy synthesis, effective secretion of rhHGF, processing mode similar to that of natural one, etc The present invention provides one exogenous gene expression process of producing gene engineering medicine
3 citations
TL;DR: Understanding the interplay between the different gene expression layers during cell differentiation and cell reprogramming is expected to improve strategies aiming at producing both pluripotent stem cell and differentiated cells.
Abstract: The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) is a major challenge for medical applications such as regenerative medicine since iPSCs can be used to generate different types of functional differentiated cells. Up to now, strategies aiming at reprogramming somatic cells have been mainly conducted toward the targeting of factors modifying gene expression at the transcriptional level. However, gene expression is a multistep process involving epigenetic and transcriptional regulators, as well as RNA binding proteins and miRNAs regulating mRNA splicing, stability or translation. The different steps of the gene expression process are physically, spatially and temporally interconnected, and each factor involved in this process may itself be controlled by other regulators within that chain of molecular mechanisms. Therefore, understanding the interplay between the different gene expression layers during cell differentiation and cell reprogramming is expected to improve strategies aiming at producing both pluripotent stem cell and differentiated cells.
3 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 2 |
| 2020 | 4 |
| 2019 | 2 |
| 2018 | 4 |
| 2017 | 2 |
| 2016 | 3 |