Topic
Bouin's Fixative
About: Bouin's Fixative is a research topic. Over the lifetime, 7 publications have been published within this topic receiving 401 citations.
Papers
TL;DR: It is demonstrated that mDF provides comparable, and in many respects superior preservation of the testes to that of BF, both for IHC staining and for detailed histopathological examination, and provides an acceptable fixative for eyes, although the quality of cellular preservation is inferior to those of DF.
Abstract: Most recent revisions of regulatory guidelines for testing effects of chemicals on reproduction recommend Bouin's fluid (BF) or a "comparable fixative" instead of formalin to preserve the morphologic detail of testes for histopathological evaluation. However, picric acid in BF is a health and safety hazard, as well as a laboratory waste disposal problem. Furthermore, use of BF is labor intensive, requiring multiple alcohol rinses to remove picric acid for optimum preservation and immunohistochemical (IHC) detection of testicular antigens that may potentially be used to identify and quantify cells and functional proteins with critical roles in spermatogenesis. Recently a modified Davidson's fluid (mDF) has been reported as an altemative to BF to fix testes for routine histopathological examination. This study compared the overall histomorphologic clarity and the immuno- and histochemical staining of testicular specimens fixed in BF and mDF. Additionally, because conventional Davidson's fixative (DF) is used routinely for optimum fixation of eyes, preservation of ocular histomorphology by DF and mDF was compared. mDF resulted in noticeably less shrinkage of the seminiferous tubules and superior overall morphologic detail compared to BF. Unlike DF, the mDF also supported excellent staining of acrosomes with periodic acid-Schiff (PAS) reagent when staging of spermatogenesis was required. IHC detection of androgen receptor and PCNA (to directly and indirectly identify Sertoli cells) as well as protein gene product 9.5 (to label spermatogonia) was Superior in mDF compared to BF-fixed specimens. For histopathological examination of the eye, apposition and preservation of rods and cones, and nuclear layers of the retina were slightly inferior with mDF compared to DF. This paper has demonstrated that mDF provides comparable, and in many respects superior preservation of the testes to that of BF, both for IHC staining and for detailed histopathological examination. It also provides an acceptable fixative for eyes, although the quality of cellular preservation is inferior to that of DF.
407 citations
TL;DR: Bentin’s fixed tissues may be unsuitable when subsequent IHC and morphometry are contemplated, and an awareness of which antibodies are suitable for use in Bouin's fixed biopsies is essential.
Abstract: Aims: To investigate the problems involved in undertaking immunohistochemistry (IHC) and nuclear morphometry using Bouin’s fixed prostate biopsies. Methods: Archival Bouin’s fixed and formalin fixed, paraffin wax embedded prostatic biopsies were immunostained for three nuclear biomarkers (minichromosome maintenance protein 2 (MCM-2), p27, and Ki-67), one membrane localised biomarker (C-erb-B2), CD34, and α methylacyl-CoA racemase (AMACR). The quality of IHC staining was compared between tissues prepared separately in both fixatives. Feulgen staining was also performed on Bouin’s fixed tissues to check its suitability for nuclear morphometry. Results: MCM-2 staining was completely negative in Bouin’s fixed tissues, whereas p27 showed more background and excess cytoplasmic staining in Bouin’s fixed versus formalin fixed tissues. C-erb-B2 showed non-specific, strong luminal cell staining in the Bouin’s fixed tissue. Feulgen staining was also very weak in Bouin’s fixed tissue. However, Ki-67, AMACR, and CD34 worked equally well in Bouin’s and formalin fixed tissues. Conclusions: Bouin’s fixed tissues may be unsuitable when subsequent IHC and morphometry are contemplated. An awareness of which antibodies are suitable for use in Bouin’s fixed biopsies is essential.
39 citations
TL;DR: These studies indicate that TH-like immunoreactive fibers and terminals are not uniformly distributed in the human hippocampal complex.
23 citations
Journal Article•
TL;DR: Pol André Bouin was a distinguished French scientist who gained international reputation at the turn of the century for his work in histology and reproductive endocrinology and his name has been retained eponymously in the fixative today known as "Bouin's fixative".
Abstract: Pol Andre Bouin was a distinguished French scientist who gained international reputation at the turn of the century for his work in histology and reproductive endocrinology. His name has been retained eponymously in the fixative he described in 1897, today known as "Bouin's fixative." The fixative that bears his name is only one of many contributions he made to academic medicine.
10 citations
TL;DR: In this paper, indirect immunohistochemistry method for paraffin-embedded mouse ovaries fixed with Bouin's Fixative is described. But the method is not suitable for the detection of specific proteins.
Abstract: Immunohistochemistry (IHC) is an efficient technique to detect cellular localizations of the proteins in paraffin-embedded tissues. It allows specific proteins to be visualized by the interaction of antibodies with an enzyme-substrate-chromogen system. Here, we describe indirect immunohistochemistry method for paraffin-embedded mouse ovaries fixed with Bouin's Fixative.
3 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2016 | 1 |
| 2006 | 1 |
| 2005 | 1 |
| 2002 | 1 |
| 1992 | 1 |
| 1990 | 1 |