Topic
alpha-Eleostearic acid
About: alpha-Eleostearic acid is a research topic. Over the lifetime, 11 publications have been published within this topic receiving 124 citations. The topic is also known as: (9Z,11E,13E)-9,11,13-Octadecatrienoic acid & alpha-ELA.
Papers
TL;DR: In this article, the active component that induces apoptosis in cancer cells, bitter gourd ethanol extract was fractionated by liquid-liquid partition and silica gel column chromatography.
Abstract: Bitter gourd ( Momordica charantia L.) pericarp, placenta, and seed extracts were previously shown to induce apoptosis in HL60 human leukemia cells. To determine the active component that induces apoptosis in cancer cells, bitter gourd ethanol extract was fractionated by liquid-liquid partition and silica gel column chromatography. Several fractions obtained by silica gel column chromatography inhibited growth and induced apoptosis in HL60 cells. Among them, fraction 7 had the strongest activity in inhibiting growth and inducing apoptosis in HL60 cells. A component that induced apoptosis in HL60 cells was then isolated from fraction 7 by another silica gel column chromatography and high-performance liquid chromatography (HPLC) using a C18 column and was identified as (9Z,11E,13E)-15,16-dihydroxy-9,11,13-octadecatrienoic acid (15,16-dihydroxy alpha-eleostearic acid). 15,16-Dihydroxy alpha-eleostearic acid induced apoptosis in HL60 cells within 5 h at a concentration of 160 microM (50 microg/mL). (9Z,11E,13E)-9,11,13-Octadecatrienoic acid (alpha-eleostearic acid) is known to be the major conjugated linolenic acid in bitter gourd seeds. Therefore, the effect of alpha-eleostearic acid on the growth of some cancer and normal cell lines was examined. alpha-Eleostearic acid strongly inhibited the growth of some cancer and fibroblast cell lines, including those of HL60 leukemia and HT29 colon carcinoma. alpha-Eleostearic acid induced apoptosis in HL60 cells after a 24 h incubation at a concentration of 5 microM. Thus, alpha-eleostearic acid and the dihydroxy derivative from bitter gourd were suggested to be the major inducers of apoptosis in HL60 cells.
64 citations
TL;DR: It is indicated that CLnA and CLA have differential effects on body fat mass and liver TAG levels in mice, compared with the control and conjugated linoleic acid and CLA respectively.
Abstract: The effects of two isomers of conjugated linolenic acid (CLnA), α-eleostearic acid (α-ESA) and punicic acid (PA), on body fat and lipid metabolism were investigated, compared with a conjugated linoleic acid (CLA) mixture (primarily cis9,trans11- and trans10,cis12-18:2) and α-linolenic acid (ALA), a non-conjugated octadecatrienoic acid, in the present study. ICR mice were fed either a control diet or one of four experimental diets supplemented with 1 % α-ESA, 1 % PA, 1 % CLA mixture and 1 % ALA in the form of triacylglycerols (TAG) for 6 weeks. The weights of perirenal and epididymal adipose tissues were significantly decreased while the liver weight was significantly increased in mice fed CLA, compared with the control. In contrast to CLA, the tissue weights in α―ESA-, PA- and ALA-fed mice were not affected. No significant differences were observed in TAG, total cholesterol, high-density lipoprotein and low-density lipoprotein cholesterol levels among the five groups. The liver TAG level was significantly decreased in mice fed α-ESA and PA while it was significantly increased in mice fed the CLA mixture. These results indicate that CLnA and CLA have differential effects on body fat mass and liver TAG levels in mice.
39 citations
Journal Article•
TL;DR: The seeds of five cucurbitaceae species growing in Niger, were subjected to a physicochemical study in order to evaluate their nutritional interest as discussed by the authors, and the results showed that they are 13-26% protein content, 9-31% lipids content, and 2 - 5% ash content (P, Na, K, Ca, Mg, Fe, Cu, Zn, Mn).
Abstract: The seeds of 5 cucurbitaceae species growing in Niger, were subjected to a physicochemical study in order to evaluate their nutritional interest. They are 13-26% protein content, 9-31% lipids content, and 2 - 5% ash content (P, Na, K, Ca, Mg, Fe, Cu, Zn, Mn). The major fatty acids of total lipids are: palmitic acid (12-18%), stearic acid (8-12%), oleic acid (13-40%), linoleic acid (31-61%). Nutritional interest was evaluated by considering 4 criteria: R1 (% lipids)/(% proteins), R2 = (%saturated FA)/(%unsaturated FA) , linoleic acid content and occurence or not of alpha eleostearic acid. This last one has not been highlighted on studied samples. So, taking into account of R1 and R2 suggests following classification, according to a decreasing scale of interest, for studied species: Cucumis melo > Cucurbita pepo > Cucumis metuliflorus > Citrullus lanatus > Lagenaria siceraria.
11 citations
TL;DR: The findings support the potential commercial viability of Njangsa seed oil as an important source of physiologically beneficial PUFAs and indicate that the acid-catalyzed transesterification of NSO resulted in lower yields of α-ESA methyl esters, due to isomerization.
Abstract: This study investigated the compositional characteristics and shelf-life of Njangsa seed oil (NSO). Oil from Njangsa had a high polyunsaturated fatty acid (PUFA) content of which alpha eleostearic acid (α-ESA), an unusual conjugated linoleic acid was the most prevalent (about 52%). Linoleic acid was also present in appreciable amounts (approximately 34%). Our investigations also indicated that the acid-catalyzed transesterification of NSO resulted in lower yields of α-ESA methyl esters, due to isomerization, a phenomenon which was not observed under basic conditions. The triacylglycerol (TAG) profile analysis showed the presence of at least 1 α-ESA fatty acid chain in more than 95% of the oil's TAGs. Shelf-life was determined by the Weibull Hazard Sensory Method, where the end of shelf-life was defined as the time at which 50% of panelists found the flavor of NSO to be unacceptable. This was determined as 21 wk. Our findings therefore support the potential commercial viability of NSO as an important source of physiologically beneficial PUFAs.
7 citations
TL;DR: In this article, the effect of Vicine and α-Eleostearic acid from the seeds of Momordica charantia was investigated and shown to be inhibitory.
Abstract: α-Glucosidase Inhibitory Effect of Vicine and α-Eleostearic Acid from the Seeds of Momordica charantia Heung Joo Yuk, Geon Min Noh, Jeong Sook Choe, Oh Kyung Kwon, Su Young Hong, Sang Soo Kang, Kye Man Cho and Dong Sik Park (Department of Agro-food Resources, National Academy of Agricultural Science, Rural Development Administration, Wanju-Gun, 565-851, Korea, Advanced Food Materials Research Institute, Kolmar BNH Inc. Sejong, 339-706, Korea, Department of Anatomy, School of Medicine, Gyeongsang National University, Jinju, 660-751, Korea, Department of Food Science, Gyeongnam National University of Science and Technology, Jinju, 660-758, Korea)
4 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2020 | 1 |
| 2019 | 1 |
| 2018 | 1 |
| 2017 | 1 |
| 2016 | 1 |
| 2015 | 1 |