Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein
Jason Tenboer,Shibom Basu,Nadia A. Zatsepin,Kanupriya Pande,Despina Milathianaki,Matthias Frank,Mark S. Hunter,Sébastien Boutet,Garth J. Williams,Jason E. Koglin,Dominik Oberthuer,Michael Heymann,Christopher Kupitz,Chelsie E. Conrad,Jesse Coe,Shatabdi Roy-Chowdhury,Uwe Weierstall,Daniel James,Dingjie Wang,Thomas D. Grant,Anton Barty,Oleksandr Yefanov,Jennifer Scales,Cornelius Gati,Carolin Seuring,Vukica Šrajer,Robert Henning,Peter Schwander,Raimund Fromme,Abbas Ourmazd,Keith Moffat,Jasper J. van Thor,John C. H. Spence,Petra Fromme,Henry N. Chapman,Marius Schmidt +35 more
TL;DR: This work used microcrystals of photoactive yellow protein as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features, which open the way to the study of reversible and nonreversible biological reactions on time scales as short as femtoseconds under conditions that maximize the extent of reaction initiation throughout the crystal.
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Abstract: Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics of biomolecules. We used microcrystals of photoactive yellow protein (a bacterial blue light photoreceptor) as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features; these allowed the determination of structures of reaction intermediates to a resolution of 1.6 angstroms. Our results open the way to the study of reversible and nonreversible biological reactions on time scales as short as femtoseconds under conditions that maximize the extent of reaction initiation throughout the crystal.
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Beyond crystallography: Diffractive imaging using coherent x-ray light sources
TL;DR: The revolutionary advances that are transforming x-ray sources and imaging in the 21st century are reviewed.
Linac Coherent Light Source: The first five years
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Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein.
Kanupriya Pande,C. Hutchison,Gerrit Groenhof,Andrew Aquila,Josef S. Robinson,Jason Tenboer,Shibom Basu,Sébastien Boutet,Daniel P. DePonte,Mengning Liang,Thomas A. White,Nadia A. Zatsepin,Oleksandr Yefanov,Dmitry Morozov,Dominik Oberthuer,Cornelius Gati,Ganesh Subramanian,Daniel James,Yun Zhao,Jake Koralek,Jennifer Brayshaw,Christopher Kupitz,Chelsie E. Conrad,Shatabdi Roy-Chowdhury,Jesse Coe,Markus Metz,Paulraj Lourdu Xavier,Thomas D. Grant,Jason E. Koglin,Gihan K. Ketawala,Raimund Fromme,Vukica Šrajer,Robert Henning,John C. H. Spence,Abbas Ourmazd,Peter Schwander,Uwe Weierstall,Matthias Frank,Petra Fromme,Anton Barty,Henry N. Chapman,Keith Moffat,Jasper J. van Thor,Marius Schmidt +43 more
TL;DR: Structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein are identified.
Direct observation of ultrafast collective motions in CO myoglobin upon ligand dissociation.
Thomas R. M. Barends,Lutz Foucar,Albert Ardevol,Karol Nass,Andrew Aquila,Sabine Botha,R. Bruce Doak,Konstantin Falahati,Elisabeth Hartmann,M. Hilpert,Marcel Heinz,Marcel Heinz,Matthias C. Hoffmann,Jürgen Köfinger,Jason E. Koglin,Gabriela Kovacsova,Mengning Liang,Despina Milathianaki,Henrik T. Lemke,Jochen Reinstein,C.M. Roome,Robert L. Shoeman,Garth J. Williams,Irene Burghardt,Gerhard Hummer,Sébastien Boutet,Ilme Schlichting +26 more
TL;DR: Time-resolved serial femtosecond crystallography at an x-ray free-electron laser is used to resolve the ultrafast structural changes in the carbonmonoxy myoglobin complex upon photolysis of the Fe-CO bond and supports the prediction that an immediate collective response of the protein occurs upon ligand dissociation.
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Protein Allostery and Conformational Dynamics.
Jingjing Guo,Huan-Xiang Zhou +1 more
TL;DR: The roles of conformational dynamics in allosteric regulation and Perspectives on contentious issues, for example, the relationship between picosecond-nanosecond local and microsecond-millisecond conformational exchange dynamics, are presented.
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