Journal Article10.1089/HYB.2006.25.133
Structural mapping of immunoglobulin subclasses using multiplexed bead flow cytometry.
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TL;DR: The sensitivity and flexibility of this approach suggests the utility of multiplexed bead flow cytometry in the early management of immunoglobulin-secreting hybridomas, and efficiently defined traditional class/subclass determinants as well as more subtle patterns of crossreactivity.
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Abstract: Monoclonal hybridomas secrete immunoglobulin molecules with a single specificity and distinct class/subclass structure. The determination of immunoglobulin structure can be used to facilitate hybridoma colony management and predict monoclonality. In this report, we used multiplexed bead flow cytometry to define hybridoma class/subclass. The assay was sufficiently sensitive to detect 50 ng/mL of antibody. The multiplexed bead assay efficiently defined traditional class/subclass determinants as well as more subtle patterns of crossreactivity. Further, the assay was combined with Poisson statistics to provide a numerical estimate of hybridoma monoclonality. The sensitivity and flexibility of this approach suggests the utility of multiplexed bead flow cytometry in the early management of immunoglobulin-secreting hybridomas.
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Citations
Hierarchical clustering of monoclonal antibody reactivity patterns in nonhuman species.
Juan P. Pratt,Qing Treitler Zeng,Qing Treitler Zeng,Dino J. Ravnic,Harold O. Huss,James D. Rawn,Steven J. Mentzer +6 more
TL;DR: It is concluded that the computational algorithm developed is a potentially useful tool for both Mab classification and molecular taxonomy in nonhuman experimental systems.
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Sydney Cohen,Rodney R. Porter +1 more
TL;DR: The structure and linkages of the four recognized chains of immunoglobulins, the positions of their various genetic markers, and the nature of the antibody-combining sites are discussed, including the existence of two separate chains within the A or heavy chain.
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Rapid Multiplex Assay for Serotyping Pneumococci with Monoclonal and Polyclonal Antibodies
TL;DR: The serotyping system can identify not only all the serotypes in pneumococcal vaccines but also most (>90%) of clinical isolates.
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Selective cloning of hybridoma cells for enhanced immunoglobulin production using flow cytometric cell sorting and automated laser nephelometry
TL;DR: The results indicate that flow cytometric-assisted single cell deposition provides positive attributes of several traditional hybridoma cloning techniques and, in addition, furnishes a tool for steering the cloning process toward selection of enhanced immunoglobulin producing cultures.
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