Precise nanometer localization analysis for individual fluorescent probes
TL;DR: A localization algorithm motivated from least-squares fitting theory is constructed and tested both on image stacks of 30-nm fluorescent beads and on computer-generated images (Monte Carlo simulations), and results show good agreement with the derived precision equation.
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About: This article is published in Biophysical Journal. The article was published on 01 May 2002. and is currently open access. The article focuses on the topics: Point spread function & Background noise.
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