Bioanalytical method Development and Validation for Estimation of Olanzapine in K3edta Human Plasma by Liquid Chromatography–Tandem Mass Spectrometry and Application to a Pharmacokinetic Study
TL;DR: A rapid and sensitive LC-MS/MS method for the bioanalytical method development and validation for estimation of olanzapine in k3EDTA human plasma using the internal standard has been developed and validated as discussed by the authors .
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Abstract: A rapid and sensitive LC–MS/MS method for the bioanalytical method development and validation for estimation of olanzapine in k3EDTA human plasma using Olanzapine D3 as internal standard has been developed and validated. The analytes and IS were extracted from plasma by solid phase extraction using Oasis HLB 1cc (30mg) Extraction Cartridge and separated on a Cosmosil, 5µm, C18 150*4. 6 mmcolumn using a 10 mM ammonium formate in Water: Acetonitrile (10: 90) at a flow rate of 1.0 mL/min. Detection involved an API-4000 LC–MS/MS with electrospray ionization in the positive ion mode and multiple-reaction monitoring for analysis. The method was validated according to FDA guidelines and shown to provide intra- and inter-day precision and accuracy within acceptable limits in a run time of only 4.0 min. The method was successfully applied to a pharmacokinetic study involving a single oral administration of a combination tablet to human male volunteers
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References
Mechanistic investigation of ionization suppression in electrospray ionization.
TL;DR: Results point to changes in the droplet solution properties caused by the presence of nonvolatile solutes as the main cause of ionization suppression in electrospray ionization of biological extracts.
1.1K
An automated screening method for drugs and toxic compounds in human serum and urine using liquid chromatography-tandem mass spectrometry.
TL;DR: The developed LC-MS-MS system seems to be a valuable alternative to other general unknown screening methods allowing fast and specific identification of drugs in serum and urine samples.
39
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TL;DR: The bioequivalence of 2 commercial 10-mg tablet formulations of olanzapine were assessed by statistical analysis of the pharmacokinetic parameters C(max), AUC from 0 to 72 hours after dosing (AUC(0-72)), and AUC( 0-infinity) as required by the Egyptian health authority for the marketing of a generic product.
23
Therapeutic drug monitoring of seven psychotropic drugs and four metabolites in human plasma by HPLC-MS.
TL;DR: Good performance in terms of recoveries, selectivity, stability, repeatability, intermediate precision, trueness and accuracy, was obtained by the developed and validated LC-MS method for simultaneous quantification of aripiprazole (ARI), atomoxetine (ATO) and their active metabolites in human plasma.
Validated HPLC-MS/MS method for determination of quetiapine in human plasma.
TL;DR: In this article, a validated, highly sensitive and selective high-pressure liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantitative determination of quetiapine (QUE) in human Na2EDTA plasma with MS detection.