Zhaohui Cai
University of Kentucky
10 Papers
126 Citations
Zhaohui Cai is an academic researcher from University of Kentucky. The author has contributed to research in topics: Hepatitis C virus & NS5B. The author has an hindex of 9, co-authored 9 publications.
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Papers
Replication of Hepatitis C Virus (HCV) RNA in Mouse Embryonic Fibroblasts: Protein Kinase R (PKR)-Dependent and PKR-Independent Mechanisms for Controlling HCV RNA Replication and Mediating Interferon Activities
Kyung-Soo Chang,Zhaohui Cai,Chen Zhang,Ganes C. Sen,Bryan R.G. Williams,Bryan R.G. Williams,Guangxiang Luo +6 more
TL;DR: It is demonstrated that a subgenomic RNA replicon of genotype 2a HCV replicated efficiently in mouse embryonic fibroblasts (MEFs), as determined by cell colony formation efficiency and the detection of HCV proteins and both positive- and negative-strand RNAs.
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Immunogenic and Functional Organization of Hepatitis C Virus (HCV) Glycoprotein E2 on Infectious HCV Virions
Zhen-Yong Keck,Jinming Xia,Zhaohui Cai,Ta-Kai Li,Ania M. Owsianka,Arvind H. Patel,Guangxiang Luo,Steven K. H. Foung +7 more
TL;DR: Findings support an immunogenic model of HCV E2 having three immunogenic domains with distinct structures and functions and provide added support for the idea that CD81 is required for virus entry.
97
Stimulation of Hepatitis C Virus (HCV) Nonstructural Protein 3 (NS3) Helicase Activity by the NS3 Protease Domain and by HCV RNA-Dependent RNA Polymerase
Chen Zhang,Zhaohui Cai,Young Chan Kim,Ranjith Kumar,Fenghua Yuan,Pei Yong Shi,C. Cheng Kao,Guangxiang Luo +7 more
TL;DR: Findings suggest that HCV RdRp regulates the functions of NS3 during HCV replication and enhances the NS3 protease domain of the full-length NS3 (NS3FL), which is contrary to a previously published report that the HCV NS3 enhances NS5B RdRP activity.
91
Effects of mutations of the initiation nucleotides on hepatitis C virus RNA replication in the cell.
TL;DR: Findings demonstrate that replication of positive-strand HCV RNA was preferentially initiated with purine nucleotides (ATP and GTP), whereas the negative- StrandHCV RNA replication is invariably initiated with an ATP.
36
Mutagenesis Analysis of the rGTP-Specific Binding Site of Hepatitis C Virus RNA-Dependent RNA Polymerase
TL;DR: It is demonstrated that the rGTP-specific binding site of the HCV NS5B is not required for in vitro RdRp activity but is important for HCV RNA replication in vivo.
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