The invention discloses a method and primer for detecting gene mutation relevant to the hereditary blood coagulation factor XI(F11). The primer comprises 15 pairs of primers with amplification detecting all-exon sequences. The Sanger sequencing technology and the sequencing primers are adopted. Hereditary blood coagulation factor XI(F11) deficiency disease all-exon and relevant mutation can be fast detected. The detecting result completed through the method and primer is accurate, diagnosis of the hereditary blood coagulation factor XI(F11) deficiency disease can be assisted, and the method and primer have important reference significance on early intervention, early treatment and antenatal diagnosis.

Method and primer for detecting hereditary blood coagulation factor XI(F11) genes

The invention provides a method for detecting cattle SMC2 gene recessive lethal mutation, and relates to the field of molecular biology. The method provided by the invention is based on a pyrosequencing technology, a pair of primers, a universal primer and a sequencing primer having the amino acid sequences as shown in SEQ ID No.1, 2, 3 and 4 respectively are adopted for analyzing single nucleotide polymorphism of a 95410507 bp locus of a cattle No.8 chromosome, and results find out that a wild-type homozygote has a T/T genotype at a mutation locus, a pyrosequencing result pattern indicates that the peak height ratio of the T base to the C base is 3:1; a lethal mutation carrier peak pattern has a T/C genotype at the mutation locus, and the pyrosequencing result pattern indicates that the peak height ratio of the T base to the C base is 5:3. The method provided by the invention is simple to operate, high in sensitivity, strong in accuracy, high in throughput and low in detection cost, and has importable application value in cattle breed-conservation and breeding processes.

Method for detecting cattle SMC2 gene recessive lethal mutation

The invention discloses a method for identifying milk production traits of dairy cows based on an ACACB gene and application thereof The method for identifying the milk production traits of the dairy cows refers to detection of the genotypes and/or the genotype of 66218726 -position deoxyribonucleotide and/or 66218117 -position deoxyribonucleotide and/or 66199005 -position deoxyribonucleotide and/or 66167817 -position deoxyribonucleotide and/or 66102564 -position deoxyribonucleotide on a chromosome 17 of a bovine genome Tests prove that the method provided by the invention is simple, convenient, rapid, sensitive, and reliable, stable and accurate in result, and can be used for assisting in identification of a cow population with excellent milk production traits, and is suitable for large-population detection large-scale laboratory detection With the method, early screening can be performed on cows to be screened, the breeding cost is reduced, and the milk production of the cows during actual production can be effectively improved, so that the method will play a huge role during cow breeding

Method for identifying milk production traits of dairy cows based on ACACB gene and application thereof

The invention provides a molecular marker for detecting DNA methylation of cow mastitis resistance and a primer pair of the molecular marker According to an amplification primer pair and a sequencing primer pair designed for the molecular marker, the DNA methylation of cow mastitis resistance can be detected The detecting method comprises the following steps: treating the genome DNA of a cow to be detected through bisulfite, performing PCR amplification on a TRAPPC9 gene by using the amplification primer pair, and sequencing by using the sequencing primer pair and a pyrophosphoric acid instrument, so as to obtain the methylation level of a TRAPPC9 gene promotor region of the cow to be detected According to the invention, a theory basis and a hereditary basis are provided for molecular breeding of cows with mastitis resistance

Molecular marker for detecting DNA methylation of cow mastitis resistance

The invention discloses a primer composition for detecting a harmful gene of cattle blood coagulation factor XI deficiency, a kit with the primer composition and an application of the kit. The primer composition disclosed by the invention is composed of a primer group A and a primer group B, wherein the primer group A is composed of a primer 1 and a primer 2, the primer group B is composed of a primer 3 and a primer 4, and the nucleotide sequences of the primer 1, the primer 2, the primer 3 and the primer 4 are respectively shown as SEQ ID NO. 1-4. The invention also provides the kit with the primer composition. The method for applying the kit disclosed by the invention to the detection of the harmful gene of the cattle blood coagulation factor XI deficiency comprises the steps of extracting the complete set of DNA (Deoxyribonucleic acid) in cattle blood as a template to carry out nested PCR (Polymerase Chain Reaction) amplification to obtain a PCR product, and carrying out gel electrophoresis on the obtained PCR product so as to directly know about the basic group change on a mutation site according to an electrophoresis result and sequence the PCR product, thereby ensuring the accuracy of the result and meeting the requirements of a detecting technology for characteristics such as high speed, precision, high throughput and the like.

Primer composition for detecting harmful gene of cattle blood coagulation factor XI deficiency, kit with primer composition and application of kit

The invention discloses a molecular marker related to milk production traits of dairy cows and an application of the molecular marker and discloses a group of primers. The primers comprise DNA molecules represented by (1)-(2) and/or (3)-(4) as follows: (1) DNA molecules represented by SEQ ID No.5; (2) DNA molecules represented by SEQ ID No.6; (3) DNA molecules represented by SEQ ID No.7; (4) DNA molecules represented by SEQ ID No.8. The molecular marker can be applied to assistance in identification of cow groups with the excellent milk production traits and has the advantages as follows: convenience, quickness, sensitivity, reliable, stable and accurate result and capability of meeting the large-group-size examination requirement of a laboratory.

Molecular marker related to milk production traits of dairy cows and application of molecular marker

The invention provides a kit for detecting 15bp insertion mutation in a ninth exon of a bovine coagulation factor XI gene. The kit comprises a primer combination shown as SEQ ID NO. 1-4. Based on the position of a 15bp insertion fragment in the ninth exon of the bovine coagulation factor XI gene, two pairs of primers across a 15bp insertion fragment area are designed, based on which a PCR (Polymerase Chain Reaction) detection system is established. The first pair of primers (Seq ID No. 1 and 2) only specifically amplify a wild type allele, and the second pair of primers (Seq ID No.3 and 4) only specifically amplify a mutant allele; the length difference between two PCR products is 94bp, different genotypes can be sensitively distinguished by shorter-timer electrophoresis by using low-concentration agarose gel, and thus carriers of genetic defects are accurately screened out.

Kit for detecting 15bp insertion mutation in ninth exon of bovine coagulation factor XI gene

The invention provides a molecular marker for detecting resistant DNA (Deoxyribose Nucleic Acid) methylation of dairy cow mastitis and a primer pair of the molecular marker. According to an amplification primer pair and a sequencing primer pair designed by the molecular marker, the resistant DNA methylation of the dairy cow mastitis can be detected. The detecting method comprises the following steps of: treating genome DNA of a to-be-detected dairy cow by bisulfate; carrying out PCR (Polymerase Chain Reaction) amplification onto a CD4 gene by utilizing the amplification primer pair; sequencing by utilizing the sequencing primer pair to match with a pyrophosphoric acid apparatus, so that the methylation degree of a CD4 gene promoter region of the to-be-detected dairy cow can be detected. The molecular marker for detecting resistant DNA methylation of dairy cow mastitis disclosed by the invention can be used for providing theoretical basis and hereditary basis for molecular breeding of the dairy cow with the mastitis.

Molecular maker for detecting resistant DNA (Deoxyribose Nucleic Acid) methylation of dairy cow mastitis

Fluorescent automated detection primer and kit of pathogenic sites of Arachnomelia syndrome (AS) in Simmental cattle

Popular Tasks used by Researchers

How can I help with your research?

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

We encountered an error while loading the panel. Please try refreshing the page.

Something went wrong

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

Anyone with the link can view this chat. You can switch it back to private anytime.

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

Sun Dongxiao

Author Tools

Chat about Author