http://www.comphys.ethz.ch/hans/p/502.pdf

Fragmentation and restructuring of soft-agglomerates under shear.

Kinetics of adsorption of high molecular weight anionic polyacrylamide onto kaolinite: the flocculation process.

Experimental analysis of floc size distributions in a 1-L jar under different hydrodynamics and physicochemical conditions

In this work we present experimental and simulation analysis of the breakage and restructuring of colloidal aggregates in dilute conditions under shear. In order to cover a broad range of hydrodyna...

Experimental and modeling study of breakage and restructuring of open and dense colloidal aggregates.

In this work we investigated whether the type of shear flow, to which cells are exposed, influences the initiation of cell death. It is shown that mammalian cells, indeed, distinguish between discrete types of flow and respond differently. Two flow devices were employed to impose accurate hydrodynamic flow fields: uniform steady simple shear flow and oscillating extensional flow. To distinguish between necrotic and apoptotic cell death, fluorescence activated cell sorting and the release of DNA in the culture supernatant was used. Results show that Chinese Hamster Ovaries and Human Embryonic Kidney cells will enter the apoptotic pathway when subjected to low levels of hydrodynamic stress (around 2.0 Pa) in oscillating, extensional flow. In contrast, necrotic death prevails when the cells are exposed to hydrodynamic stresses around 1.0 Pa in simple shear flow or around 500 Pa in extensional flow. These threshold values at which cells enter the respective death pathway should be avoided when culturing cells for recombinant protein production to enhance culture longevity and productivity.

Induction of mammalian cell death by simple shear and extensional flows

Preflocculated ferric hydroxide flocs were subjected to either a simple shear flow or a two-dimensional straining flow, and their motion was optically observed. Digital image analysis was applied to extract information on orientation and deformation from the digitized frames. It was found that the simple shear flow led to a rotation of the flocs whose motion can be understood from the behavior of a solid ellipsoid. In the extensional flow, no continuous rotation occurred and flocs were broken apart along the axis of straining. The rupture forces estimated from an ellipsoid model were found to be in the range of 0.1 N/m(2). Copyright 2000 Academic Press.

Flocs in Shear and Strain Flows.

The velocity data obtained by many 3D measurement methods such as particle tracking velocimetry (PTV) are not regularly distributed in3D space. We revised three numerical schemes to interpolate the scattered velocity vectors to a regularly spaced grid. Additionally, two techniques were examined to smooth the resulting flow field. The different algorithms were tested for a synthetic data set based on the analytical solution of Burgers' vortex. To study the impact of measurement errors a Gaussian noise was superimposed on the exact solution. It was found that an interpolation scheme of higher order does not necessarily perform better than one of lower order. The most‘robust’ algorithm was used to process 3D PTV data, which were obtained from measurements of a separating flow in a forward facing step configuration. Information on the 3D streamlines and vortex structures was obtained.

Interpolation of Scattered 3D PTV Data to a Regular Grid

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Flocs in Shear and Strain Flows.

Interpolation of Scattered 3D PTV Data to a Regular Grid