The invention discloses a butterfly orchid tissue culture seedling breeding method which includes the steps: (1) inoculating butterfly orchid pedicel sections with dormant buds onto a cluster bud induction medium; (2) cutting the cluster buds, and inoculating the cut cluster buds into a proliferation medium, culturing the cluster buds by an LED (light-emitting diode) red light source, and transferring the cluster buds to culture the cluster buds by a natural light source; (3) dividing the cluster buds into single buds, inoculating the single buds into a root tip induction medium, and culturingthe single buds by an LED composite light source; (4) transmitting the root tip induction medium into a greenhouse with a sunshade net to exercise seedlings by natural light; (5) wrapping a butterflyorchid plantlet root systems with sphagna, and placing butterfly orchid plantlets into the greenhouse to culture the plantlets; (6) beginning to apply fertilizers when new roots grow out; (7) changing the sphagna into nutrition pots with the sizes of 3.5 inches when the sphagna are completely wound by the root systems; (8) applying the fertilizer once every ten days after the pots are replaced for ten days. The method for breeding the butterfly orchid tissue culture seedlings can effectively solve the problems that the reproduction rate of the cluster buds is low, rooting rate is low, butterfly orchid plants are yellowed as roots are easily rotted after the plants are transplanted and the like, and the production cost of the butterfly orchid tissue culture seedlings can be reduced.

Butterfly orchid tissue culture seedling breeding method

The invention provides a method for acquiring pepper pollen. The method comprises the steps of 1) collecting pepper male flowers which have whitish buds and do not bloom; 2) picking anthers off the pepper male flowers; 3) acquiring pollen from the anthers via a pollen collecting box: placing the anthers in the pollen collecting box, closing the box body, and keeping the box body at a proper temperature and humidity to promote the anthers to scatter pollen; 4) taking out a paper sheet, sieving a mixture on the paper sheet and obtaining the pepper pollen. With the method, high quality pepper pollen can be obtained and high yield of pepper pollen can be guaranteed; the method solves the problems of insufficient pollen production and reduction of pollen viability caused by technical restriction and weather influence in conventional pepper hybrid seed production.

A method for acquiring pepper pollen

The invention provides a disinfection method for pedicel buds of phalaenopsis amabilis, which comprises the steps of: 1) selecting phalaenopsis amabilis plants which have complete pedicel buds and remains pedicels with the pedicel buds; 2) wiping the pedicels with alcohol cotton balls for 3-5 times from bottom to top; 3) according to the aged degree of the pedicels, classifying the pedicels into aged pedicels and tender pedicels; 4) preparing three disinfectants, respectively placing the aged pedicels and tender pedicels into two empty sterile bottles, pouring 1# disinfectant into the bottles with the aged pedicels and the 2# disinfectant into the bottles with the tender pedicels, and then pouring the disinfectants out; 5) stripping off the bracts on the pedicel buds on the aged pedicels and the tender pedicels after the first disinfection, soaking the pedicel buds, after the bracts are stripped off, in the 3# disinfectant, and pouring the disinfectant out; 6) inoculating the pedicel buds in a culture bottle filled with a pedicel bud inductive culture medium; and 7) placing the culture bottle in a culture room and performing induction to the pedicel buds. The disinfection method is 2-7% in pollution ratio of the pedicel buds, is 95-99% in survival rate of the pedicel buds, and is low in cost of the disinfectants.

Disinfection method for pedicel buds of phalaenopsis amabilis

The invention relates to a sterilization method for cut bud explants in Chinese orchid stem tip tissue culture. The method comprises the following steps: (1) selecting explants; (2) pretreating the explants; (3) cutting and drying the explants without direct sunshine; (4) sterilizing the explants twice; (5) cutting and cultivating after sterilization is completed. The sterilization method for cutbud explants in Chinese orchid stem tip tissue culture is applicable to all Chinese orchid varieties, the survival rate of the explants can be greatly increased while pollution rates and browning rates are reduced, and the sterilization method has great popularization and application prospects and has high economic benefits.

Sterilization method for cut bud explants in Chinese orchid stem tip tissue culture

The invention discloses a butterfly orchid pollen storage and breeding method. A cap, a pollen load stem and a sticky tray of a butterfly orchid pollen are taken off, adhered in a pollen collecting box through the adhesiveness of the sticky tray, marked, and classified and stored according to the different varieties, can be used on the same day, can be stored for 2 months if being refrigerated ina refrigerator, and can be stored for months if stored at minus 20 DEG C. The flowering of a hybridized seedling is induced when the seedling is 2.5 inches on the field cultivation stage, the good-inflorescence seedling with flower color meeting the breeding objective is chosen to rapidly propagate, and the breeding time of the seedling is shortened by 5 months. The small test seedling obtained after rapid propagation is planted in a greenhouse, the flower forcing is started after the small seedling is planted to 1.7 inches and the moderate seedling is planted to 2.8 inches, the specificity, the consistency and the stability are tested after the flowering, the time of about 5 months is also shortened, and the whole flow shortens the breeding time of a new variety of the butterfly orchid.

Butterfly orchid pollen storage and breeding method

The invention discloses a method for propagating plants rapidly. The method comprises the following steps that butterfly orchid pedicle auxiliary buds after flowering are induced to form nutritional buds, and then the nutritional buds are cut, and the abandoned pedicle stem segments act as explants, and are directly inoculated into an induction culture medium for inducing clustered buds; the novel induced buds are cut off to be inoculated to the propagation culture medium for subculture propagation culture; finally rooting and strenghening are carried out and tissue culture seedlings are transplanted. By the method, the auxiliary buds on the butterfly orchid pedicles after flowering and abandoned pedicle materials after bud cutting germinate in a manually prepared culture medium for multiple times to generate novel buds. The method has the advantages that not only is the normal flower period of butterfly orchid not affected, but also butterfly orchid mother plants are not hurt, the butterfly orchid pedicle stem segments are recycled for 2-3 times; pedicle materials are used fully, waste is turned into wealth; the propagation velocity and the production of butterfly orchid tissue culture seedlings are improved greatly; the produced seed seedlings are strong and neat, and are convenient to manage in a factory.

Method for inducing clustered shoots to rapidly propagate butterfly orchid by utilizing pedicle stem segments after bud cutting

The utility model discloses a marigold pollen collecting device. The device comprises a pollen collector and an induced draft fan, wherein the pollen collector comprises a box body and a box cover, a pollen filter is arranged between the pollen body and the box cover, a pollen collecting chamber is arranged between the pollen filter and the box body, a negative pressure chamber is arranged between the pollen filter and the box cover, a pollen suction pipe communicated with the pollen collecting chamber is arranged outside the box body, an impurity filtering cap is arranged on an outer port of the pollen suction pipe, a negative pressure pipe which is communicated with the negative pressure chamber and also serves as a handle is arranged outside the box cover, and the negative pressure pipe is communicated with the induced draft fan through a hose. The device is simple in structure, convenient to carry, high in pollen collecting efficiency and suitable for being applied to large-area marigold hybrid seed production.

Marigold pollen collecting device

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Method for inducing clustered shoots to rapidly propagate butterfly orchid by utilizing pedicle stem segments after bud cutting

Marigold pollen collecting device