Ming Ding

TL;DR: In this article, a tri-anti sandwich enzyme-linked immuno sorbent assay (TAS-ELISA) was presented, which consists of positive control, negative control, a sorghum mosaic virus polyclonal antibody, an enzyme labeled antibody and other materials and drugs.

TL;DR: In this paper, a method for detecting different strains of potato virus Y tobacco isolates was proposed, which comprises the following steps of: trapping the potato virus in an infected plant sample by using a potato virus specific antibody; performing high-temperature transformation to obtain a virus RNA as a template; performing one-step method reverse transcriptase polymerase chain reaction (one-step RT-PCR) by using degenerate primers of coat protein genes of different strain of the Potato virus Y; performing enzyme digestion on a PCR product, and detecting a target fragment through electroph

TL;DR: In this article, a method for rapidly detecting a tomato spotted wilf virus in thrips is proposed, which comprises the following steps: putting one or more thrips to be detected onto a nitrocellulose membrane which is separated via drawing, adding 2 microliter of sample processing buffer solution, grinding the thrips in the buffer solution by using a pipette tip, processing different polypide samples in sequence, sucking 2 micro-liter of positive reference solution and 2 micro liter of negative reference solution, and dripping onto the nitroglobalose membrane, after the

TL;DR: In this article, a rice dwarf virus tri-anti sandwich enzyme-linked immuno sorbent assay kit was presented, which comprises positive control, negative control, a rice dwarfish virus polyclonal antibody, an enzyme-labeled antibody and other materials and drugs, including buffered solution, PBST, virus extraction buffer, sealing buffer, substrate buffer solution, a substrate, 5 enzyme label plates and 20 disposable droppers.