The invention provides an extraction and purification method and application of a fermented astragalus polysaccharide. The extraction and purification method of the fermented astragalus polysaccharide comprises the following steps of (1) carrying out warm immersion extraction on fermented astragalus, concentrating an extracting solution by using a membrane intercepting ultrafilter, removing protein through a Sevag method, and carrying out dialysis, alcohol precipitation and washing to obtain a crude fermented astragalus polysaccharide; (2) purifying the fermented astragalus polysaccharide by adopting DEAE Sepharose FastFlow, eluting by using ultrapure water, concentrating and freeze-drying an eluant to obtain a neutral polysaccharide DF-1, eluting by using gradient NaCl, and concentrating, dialyzing, concentrating and freeze-drying the eluant to obtain a weakly acidic polysaccharide; (3) purifying the neutral polysaccharide DF-1 obtained from the step (2) by adopting a Sephacryl S-400 High Reslution preparation chromatographic column, eluting by using ultrapure water, concentrating and freeze-drying the eluant to obtain neutral polysaccharides SF-1 and SF-2. Compared with a crude drug APS, the FAPS (Fermented Astragalus Polysaccharide) extracted by applying the extraction and purification method provided by the invention is outstandingly increased in yield and content.

Extraction and purification method and application of fermented astragalus polysaccharide

The invention relates to a preparation method of white muscardine silkworm anticancer-activity polysaccharide BBPW-2, which comprises the following steps: degreasing white muscardine silkworm powder under reflux of acetone-petroleum ether and 80% ethanol to remove glycosides and alkaloids, and extracting with distilled water at 80-100 DEG C to obtain crude polysaccharide; and after removing proteins from the crude polysaccharide by a Sevag process, separating out the neutral polysaccharide component by DEAE (diethylaminoethanol) sepharose ion-exchange chromatography, carrying out propylene dextrangel S-300 chromatography, taking the second eluting peak, concentrating under reduced pressure, carrying out propylene dextrangel S-200 chromatography for further purification, and carrying out freeze-drying to obtain the white muscardine silkworm anticancer-activity polysaccharide BBPW-2. The product prepared by the method provided by the invention has uniform purity, and the molecular weight is 2.0*10 Da; and the product has an inhibiting action on growth of human cervical cancer cells Hela and human liver cancer cells HepG2, does not have any adverse effect on growth of normal human embryo kidney cells HEK293 and mouse macrophages RAW264.7, and can be used for developing anticancer products.

Preparation method of white muscardine silkworm anticancer-activity polysaccharide BBPW-2

Isolates of hedysari polybotrys polysaccharide 3 and application thereof

The invention discloses a medium-molecular weight segment Astragalus polysaccharide and a preparation method and application thereof, and belongs to the technical field of biomedical manufacture. Themedium-molecular weight segment Astragalus polysaccharide is obtained through hydrolysis of crude polysaccharide of Astragalus membranaceus, and the molecular weight of the medium-molecular weight segment Astragalus polysaccharide is in the range of 103-104 Daltons. The invention also discloses the preparation method of the medium-molecular weight Astragalus polysaccharide, and the preparation method comprises the step of performing acid hydrolysis on crude polysaccharide of Astragalus membranaceus so as to obtain the medium-molecular weight segment Astragalus polysaccharide. The invention also discloses application of the medium-molecular weight segment Astragalus polysaccharide in preparation of drugs or food for improving intestinal mucosal immunity and functions of the intestinal micro-ecological system, the medium-molecular weight segment Astragalus polysaccharide produced through hydrolysis of Astragalus polysaccharide has a better effect on regulation of intestinal mucosal immunity and the intestinal micro-ecological system than natural Astragalus polysaccharide, and the health care effect of Astragalus polysaccharide can be enhanced. The acid hydrolysis process of Astragalus polysaccharide is simple, and has easy industrial production.

Medium-molecular weight segment Astragalus polysaccharide and preparation method and application thereof

The invention discloses a low-molecular-weight glucan, characterized in that the glucan is obtained by separating from radix astragali. The invention also discloses a method for separating and purifying the low-molecular-weight glucan. In addition, the invention discloses a use of the low-molecular-weight glucan in the preparation of a medicament for the immunopotentiation and the adjutant therapy of tumor or a health food.

Low-molecular-weight glucan, its preparation method and use

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Low-molecular-weight glucan, its preparation method and use