M. N. Widjojoatmodjo
Utrecht University
9 Papers
420 Citations
M. N. Widjojoatmodjo is an academic researcher from Utrecht University. The author has contributed to research in topics: Single-strand conformation polymorphism & Immunomagnetic separation. The author has an hindex of 8, co-authored 9 publications.
Chat about Author
Papers
The magnetic immuno polymerase chain reaction assay for direct detection of salmonellae in fecal samples.
TL;DR: The magnetic immuno PCR assay (MIPA), which combines immunomagnetic separation by using specific monoclonal antibodies and PCR, was used to directly detect salmonellae in feces from humans.
274
Rapid identification of bacteria by PCR-single-strand conformation polymorphism.
TL;DR: PCR-SSCP is a simple and rapid method for the detection and identification of a wide spectrum of bacteria by whole-cell-based PCR amplification with the use of conserved primers and identification by nondenaturing gel electrophoresis.
150
Evaluation of the magnetic immuno PCR assay for rapid detection of Salmonella.
TL;DR: The MIPA specifically detected 100 cfu of Salmonella within 5 h and may be potentially useful for rapid detection ofSalmonella in clinical specimens and food.
140
Rapid detection of salmonellae in poultry with the magnetic immuno-polymerase chain reaction assay.
TL;DR: Data indicate that the MIPA developed for S. typhimurium is applicable to other commonly isolated serotypes, and the use of immunoseparation with a Salmonella serogroup B-specific monoclonal antibody improved the sensitivity, but enrichment was required for the detection of low levels of contamination.
129
Comparison of immunomagnetic beads coated with protein A, protein G, or goat anti-mouse immunoglobulins. Applications in enzyme immunoassays and immunomagnetic separations.
TL;DR: PA- beads were superior to both PG- and G alpha M-beads in a magnetic enzyme immunoassay for the detection of mouse immunoglobulins as an alternative to a conventional capture ELISA and for specific isolation of bacteria from heterogeneous suspensions by immunomagnetic separation.
63