M. Cassano
10 Papers
M. Cassano is an academic researcher. The author has contributed to research in topics: Medicine & Biology. The author has an hindex of 2, co-authored 7 publications.
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Papers
High-plex protein and whole transcriptome co-mapping at cellular resolution with spatial CITE-seq
Yang Liu,Marcello DiStasio,Graham Su,Hiromitsu Asashima,Archibald Enninful,Xiaoyu Qin,Yanxiang Deng,Jungmin Nam,Fu Gao,Pino Bordignon,M. Cassano,Mary M. Tomayko,Ming Xu,Stephanie Halene,Joseph E. Craft,David A. Hafler,Rong Fan +16 more
TL;DR: In this article , the authors extended co-indexing of transcriptomes and epitopes (CITE) to the spatial dimension and demonstrated high-plex protein and whole transcriptome co-mapping.
Fully Automated Sequential Immunofluorescence (seqIF) for Hyperplex Spatial Proteomics
Francois Rivest,Deniz Eroglu,Benjamin Pelz,Joanna Kowal,Maria-Giuseppina Procopio,Pino Bordignon,Lorenzo Bruno,Matthieu Ruegg,Gabriel Campargue,Gilles Casqueiro,Lionel Arn,Saška Brajkovic,M. Cassano,Diego Gabriel Dupouy +13 more
TL;DR: Sequential ImmunoFluorescence (seqIF) as mentioned in this paper is a multiplexed protein biomarker detection method on the COMET instrument, which uses successive applications of antibody incubation and elution, and in-situ imaging enabled by an integrated microscope and a microfluidic chip.
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Abstract 1716: Mapping the cellular architecture of the tumor microenvironment by integrating hyperplex immunofluorescence and automated image analysis
P. M. Almeida,Francois Rivest,Quentin Juppet,Joanna Kowal,Benjamin Pelz,M. Cassano,Deniz Eroglu,Diego Gabriel Dupouy +7 more
TL;DR: This work demonstrates here how the combination of hyperplex images obtained using the COMET™ platform, along with machine learning clustering analysis, results in an easy workflow for analyzing the complex TME and obtaining a single-cell atlas of tissue specimens.
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842 Development of a multiplex test for predicting response to combined immunotherapies in patients with metastatic melanoma
Gabriele Madonna,P. M. Almeida,Mariaelena Capone,Vito Vanella,Lucia Festino,Antonio Sorrentino,M. Cassano,Benjamin Pelz,Diego Gabriel Dupouy,Paolo A. Ascierto +9 more
- 01 Nov 2022
TL;DR: Wang et al. as discussed by the authors studied the predictive value of a multi-parameter spatial signature composed of lymphocyte activation gene-3 (LAG-3), programmed death-ligand 1 (PD-L1), and cluster of differentiation 8 (CD8) in a retrospective cohort of patients with metastatic melanoma treated with a combination of ICI.
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1459 Characterization of tumor budding and the tumor microenvironment in colorectal cancer using hyperplex immunofluorescence
Cansaran Saygili Demir,Mauro Gwerder,Deniz Eroglu,M. Cassano,Maria-Giuseppina Procopio,Joanna Kowal,Diego Gabriel Dupouy,Inti Zlobec +7 more
- 01 Nov 2022
TL;DR: In this paper , the authors developed a sequential immunofluorescence (seqIF)-based panel for a more spatial approach to characterizing TBs and the tumor microenvironment, which allowed the visualization of inter-and intra-tumoral heterogeneity of stained cases and the identification of cell populations including immune cells, cancerassociated fibroblasts, and tumor cells.