Liqin Wang
20 Papers
46 Citations
Liqin Wang is an academic researcher. The author has contributed to research in topics: Gene & Oocyte. The author has an hindex of 9, co-authored 16 publications.
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Papers
Disruption of the sheep BMPR-IB gene by CRISPR/Cas9 in in vitro-produced embryos.
TL;DR: The results indicated that the Cas9/sgRNA system is a simple and efficient tool that may potentially be used in the genetic modification of the sheep BMPR-IB gene in vivo.
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Selection of ovine oocytes by brilliant cresyl blue staining.
TL;DR: Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects, and improving the in vitro culture efficiency of oocytes can provide more materials for similar studies.
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Highly efficient generation of transgenic sheep by lentivirus accompanying the alteration of methylation status.
Chenxi Liu,Liqin Wang,Wenrong Li,Xuemei Zhang,Yongzhi Tian,Ning Zhang,Sangang He,Tong Chen,Juncheng Huang,Mingjun Liu +9 more
TL;DR: This work illustrated for the first time that generation of transgenic sheep by injecting recombinant lentiviral transgene into zygote could be an efficient tool to improve sheep performance by genetic modification.
Expression of 2A peptide mediated tri-fluorescent protein genes were regulated by epigenetics in transgenic sheep.
Yongzhi Tian,Wenrong Li,Liqin Wang,Chenxi Liu,Jiapeng Lin,Xuemei Zhang,Ning Zhang,Sangang He,Juncheng Huang,Bin Jia,Mingjun Liu +10 more
TL;DR: The results showed that 2A peptide based tricistronic construct was subjected to hypermethylation in transgenic sheep and the silencing could be relieved by treating with methytransferase inhibitor and/or deacetylase inhibitor.
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Derivation and characterization of ovine embryonic stem‐like cell lines in semi‐defined medium without feeder cells
Yuncheng Zhao,Jiapeng Lin,Liqin Wang,Bo Chen,Chuan Zhou,Tong Chen,Meiying Guo,Sangang He,Ning Zhang,Chenxi Liu,Mingjun Liu,Juncheng Huang +11 more
TL;DR: To the authors' knowledge, this is the first experiment to use semi-defined medium without feeder-cells to derive ES-like cells from ovine blastocysts, and opens the door to deriving authentic ES cells from domesticated ungulates.
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