Chitooligosaccharide (COS), which is acknowledged for possessing multiple functions, is a kind of low-molecular-weight polymer prepared by degrading chitosan via enzymatic, chemical methods, etc. COS has comprehensive applications in various fields including food, agriculture, pharmacy, clinical therapy, and environmental industries. Besides having excellent properties such as biodegradability, biocompatibility, adsorptive abilities and non-toxicity like chitin and chitosan, COS has better solubility. In addition, COS has strong biological functions including anti-inflammatory, antitumor, immunomodulatory, neuroprotective effects, etc. The present paper has summarized the preparation methods, analytical techniques and biological functions to provide an overall understanding of the application of COS.

https://www.mdpi.com/1422-0067/19/8/2197/pdf

A Review of the Preparation, Analysis and Biological Functions of Chitooligosaccharide.

Chitosan is increasingly used for safe nucleic acid delivery in gene therapy studies, due to well-known properties such as bioadhesion, low toxicity, biodegradability and biocompatibility. Furthermore, chitosan derivatization can be easily performed to improve the solubility and stability of chitosan–nucleic acid polyplexes, and enhance efficient target cell drug delivery, cell uptake, intracellular endosomal escape, unpacking and nuclear import of expression plasmids. As in other fields, chitosan is a promising drug delivery vector with great potential for the fish farming industry. This review highlights state-of-the-art assays using chitosan-based methodologies for delivering nucleic acids into cells, and focuses attention on recent advances in chitosan-mediated gene delivery for fish biotechnology applications. The efficiency of chitosan for gene therapy studies in fish biotechnology is discussed in fields such as fish vaccination against bacterial and viral infection, control of gonadal development and gene overexpression and silencing for overcoming metabolic limitations, such as dependence on protein-rich diets and the low glucose tolerance of farmed fish. Finally, challenges and perspectives on the future developments of chitosan-based gene delivery in fish are also discussed.

/pdf/chitosan-based-drug-delivery-system-applications-in-fish-2h5v5n4eun.pdf

Chitosan-Based Drug Delivery System: Applications in Fish Biotechnology.

This work aimed to appraise growth performance, immune responsiveness, bacterial disease resistance and mRNA expression of two antimicrobial peptides in Labeo rohita fingerlings fed diets supplemented with seaweed Sargassum wightii and its fucoidan-rich extract. Two hundred and twenty-five fingerlings were divided into five experimental groups in triplicates, each replicate having 15 fish in a 90-L tank. Different groups were fed with isonitrogenous (35% crude protein) and isoenergetic (1408.16 kJ/100 g) diets containing either 0% fucoidan-rich seaweed extract (FRSE), 1% FRSE, 2% FRSE, 3% seaweed powder and 6% seaweed powder for 60 days. Partial nucleotide sequences of the antimicrobial peptides, hepcidin and defensin, were characterized in Labeo rohita fingerlings, and gene expression in the different tissues of experimental fish was studied. The fish-fed FRSE-supplemented diets showed significant increase (P < 0.05) in the mRNA expression of antimicrobial peptides in liver, skin and intestine tissues. Bacterial challenge study using Aeromonas hydrophila showed higher survival in the FRSE-fed groups. Fish-fed FRSE-supplemented diets also showed better non-specific immune response (NBT reduction, serum lysozyme activity, serum albumin: globulin ratio and phagocytic activity) in pre-challenge and post-challenge periods. No significant difference was observed in the growth performance in the different experimental groups. The study indicates that although dietary FRSE is a potential immunostimulant in Labeo rohita fingerlings, it does not improve growth performance.

/pdf/effect-of-dietary-sargassum-wightii-and-its-fucoidan-rich-2lfoluw5s7.pdf

Effect of dietary Sargassum wightii and its fucoidan-rich extract on growth, immunity, disease resistance and antimicrobial peptide gene expression in Labeo rohita

Analysis of the transcriptomic profilings of Mandarin fish (Siniperca chuatsi) infected with Flavobacterium columnare with an emphasis on immune responses

In this work, the preparation, characterization and removal capabilities of a novel biomass derived BC and its BCN nanocomposites are described. Possessing hierarchically porous structures, extremely large surface areas and special chemical bonds, porous BCN nanosheets have demonstrated advantages in terms of their adsorption and photocatalytic activities. The adsorption and photocatalytic activities of the as-prepared catalysts were evaluated by the degradation of RhB. The best results exhibited 97% and 95% decomposition of RhB which were obtained by using porous BCN-40 nanosheets within 120 min at 25 °C under UV light and visible light (>420 nm) irradiation respectively. The rate constant of the porous BCN-40 nanosheets for the degradation of RhB was more 16 times than that of pure h-BN. Besides, the porous BCN nanosheets showed remarkable cycling stability, maintaining a high photocatalytic activity up to 94% after 5 cycles. Furthermore, the degradation mechanisms of RhB and the photocatalytic mechanism have been explained in this paper.

/pdf/renewable-biomass-derived-porous-bcn-nanosheets-and-their-1ztfhc8acl.pdf

Renewable biomass derived porous BCN nanosheets and their adsorption and photocatalytic activities for the decontamination of organic pollutants

The immunostimulating effects of oligochitosan have been proven in several fish, however, the mechanisms underlying the stimulation are not characterized. In the present study, the effects of oligochitosan were investigated using macrophages isolated from blunt snout bream (Megalobrama amblycephala). The results showed that the phagocytic activity of the macrophages was enhanced by the addition of oligochitosan in vitro and in vivo. The two of the most important antimicrobial pathways of macrophages, NADPH oxidase and iNOS pathways were included for further studies. The amounts of superoxide anion and the mRNAs of the five subunits of NADPH oxidase genes were significantly enhanced in the oligochitosan-treated macrophages and macrophages isolated from fish fed with feed containing oligochitosan. In addition, the NO production, iNOS activity and iNOS gene expression were all significantly increased in the presence of oligochitosan. Furthermore, the mRNA levels of the TNF-α and IL-1β were also significantly increased in the macrophages derived from fish fed with oligochitosan. In conclusion, the stimulation effects of oligochitosan on the phagocytic activity of the fish macrophages were associated with respiratory burst coupled with nitric oxide production.

Oligochitosan stimulated phagocytic activity of macrophages from blunt snout bream (Megalobrama amblycephala) associated with respiratory burst coupled with nitric oxide production

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Oligochitosan stimulated phagocytic activity of macrophages from blunt snout bream (Megalobrama amblycephala) associated with respiratory burst coupled with nitric oxide production