A Streptomyces-like actinomycete strain, designated as YIM 78087T, was isolated from a sediment sample collected from Hehua hot spring in Tengchong, Yunnan province, south-west China The taxonomic position of strain YIM 78087T was investigated by a polyphasic approach Phylogenetic analyses based on 16S rRNA gene sequences showed that strain YIM 78087T belongs to the genus Streptomyces and is closely related to Streptomyces fimbriatus DSM 40942T, Streptomyces marinus DSM 41968T and Streptomyces qinglanensis DSM 42035T (9718, 9705 and 971 % similarity, respectively) Combined with the low values of DNA–DNA hybridization between strain YIM 78087T and its closest neighbours, these analyses indicated that this new isolate represents a different genomic species in the genus Streptomyces The predominant menaquinones of strain YIM 78087T were identified as MK-9 (H4) and MK-9 (H6) The major fatty acids were identified as anteiso-C15:0 (284 %), anteiso-C17:0 (230 %) and iso-C16:0 (151 %) The whole-cell hydrolysates found to contain glucose, mannose and ribose The DNA G+C content was determined to be 730 mol% Based on the comparative analysis of phenotypic and genotypic characteristics, it is proposed that strain YIM 78087T represents a novel species of the genus Streptomyces, for which the name Streptomyces calidiresistens sp nov, is proposed The type strain is YIM 78087T (=BCRC 16955T=DSM 42108T=JCM 19629T)

Streptomyces calidiresistens sp. nov., isolated from a hot spring sediment.

A novel actinobacterial strain, designated SYSU K10005T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic and Gram-stain-positive. On the basis of 16S rRNA gene sequence analysis, strain SYSU K10005T was most closely related to the type strains of the genus 
Nonomuraea
, and shared highest sequence similarity of 98.4 % with 
Nonomuraea candida
HMC10T. DNA–DNA hybridization values between the two strains were less than 70 %. The whole-cell hydrolysates of strain SYSU K10005T contained meso-diaminopimelic acid (diagnostic diamino acid), and arabinose, madurose and rhamnose (whole-cell sugars). The major isoprenoid quinone was MK-9(H4), while the major fatty acids were iso-C16 : 0, 10-methyl C17 : 0, C17
 : 1ω8c and C17 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, lyso-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, an unidentified lipid, two unidentified ninhydrin-positive phosphoglycolipids and two unidentified phospholipids. The genomic DNA G+C content of strain SYSU K10005T was 64.2 mol%. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10005T can be characterized to represent a novel species of the genus 
Nonomuraea
, for which the name 
Nonomuraea
cavernae sp. nov. is proposed. The type strain is SYSU K10005T (=KCTC 39805T=CGMCC 4.7368T).

Nocardia cavernae sp. nov., a novel actinobacterium isolated from a karst cave sample.

A Gram negative, yellow pigmented, rod shaped bacterium designated as RLT was isolated from a hot water spring (90–98 °C) located at Manikaran in Northern India. The isolate grows at 60–80 °C (optimum, 70 °C) and at pH 7.0–9.0 (optimum pH 7.2). Phylogenetic analysis of 16S rRNA gene sequences and levels of DNA–DNA relatedness together indicate that the new isolate represents a novel species of the genus Thermus with closest affinity to Thermus thermophilus HB8T (99.5 %) followed by Thermus arciformis (96.4 %). A comparative analysis of partial sequences of housekeeping genes (HKG) further revealed that strain RLT is a novel species belonging to the genus Thermus. The melting G+C content of strain RLT was calculated as 68.7 mol%. The DNA–DNA relatedness value of strain RLT with its nearest neighbours (>97 %) was found to be less than 70 % indicating that strain RLT represents a novel species of the genus Thermus. MK-8 was the predominant respiratory quinone. The presence of characteristic phospholipid and glycolipid further confirmed that strain RLT belongs to the genus Thermus. The predominant fatty acids of strain RLT were iso-C17:0 (23.67 %) and iso-C15:0 (24.50 %). The results obtained after DNA–DNA hybridization, biochemical and physiological tests clearly distinguished strain RLT from its closely related species. Thus, strain RLT represents a novel species of the genus Thermus for which the name Thermus parvatiensis is proposed (=DSM 21745T= MTCC 8932T).

/pdf/thermus-parvatiensis-rl-t-sp-nov-isolated-from-a-hot-water-3afxogug72.pdf

Thermus parvatiensis RL(T) sp. nov., Isolated from a Hot Water Spring, Located Atop the Himalayan Ranges at Manikaran, India.

Thermus species are widespread in natural and artificial thermal environments Two new yellow-pigmented strains, L198T and L423, isolated from Little Hot Creek, a geothermal spring in eastern California, were identified as novel organisms belonging to the genus Thermus Cells are Gram-negative, rod-shaped, and non-motile Growth was observed at temperatures from 45 to 75 °C and at salinities of 0–20% added NaCl Both strains grow heterotrophically or chemolithotrophically by oxidation of thiosulfate to sulfate L198T and L423 grow by aerobic respiration or anaerobic respiration with arsenate as the terminal electron acceptor Values for 16S rRNA gene identity (≤ 9701%), digital DNA–DNA hybridization (≤ 327%), OrthoANI (≤ 875%), and genome-to-genome distance (013) values to all Thermus genomes were less than established criteria for microbial species The predominant respiratory quinone was menaquinone-8 and the major cellular fatty acids were iso-C15:0, iso-C17:0 and anteiso-C15:0 One unidentified phospholipid (PL1) and one unidentified glycolipid (GL1) dominated the polar lipid pattern The new strains could be differentiated from related taxa by β-galactosidase and β-glucosidase activity and the presence of hydroxy fatty acids Based on phylogenetic, genomic, phenotypic, and chemotaxonomic evidence, the novel species Thermus sediminis sp nov is proposed, with the type strain L198T (= CGMCC 113590T = KCTC XXX)

Thermus sediminis sp. nov., a thiosulfate-oxidizing and arsenate-reducing organism isolated from Little Hot Creek in the Long Valley Caldera, California

Lipolytic enzymes, esterases (EC 3.1.1.1) and lipases (EC 3.1.1.3), catalyze the hydrolysis of ester bonds between alcohols and carboxylic acids, and its formation in organic media. At present, they represent about 20% of commercialized enzymes for industrial use. Lipolytic enzymes from thermophilic microorganisms are preferred for industrial use to their mesophilic counterparts, mainly due to higher thermostability and resistance to several denaturing agents. However, the production at an industrial scale from the native organisms is technically complicated and expensive. The thermophilic bacterium Thermus thermophilus (T. thermophilus) has high levels of lipolytic activity, and its whole genome has been sequenced. One esterase from the T. thermophilus strain HB27 has been widely characterized, both in its native form and in recombinant forms, being expressed in mesophilic microorganisms. Other putative lipases/esterases annotated in the T. thermophilus genome have been explored and will also be reviewed in this paper.

/pdf/thermus-thermophilus-as-a-source-of-thermostable-lipolytic-56bsefvr65.pdf

Thermus thermophilus as a Source of Thermostable Lipolytic Enzymes.

Two thermophilic bacterial strains, designated YIM 77925T and YIM 77777, were isolated from two hot springs, one in the Hydrothermal Explosion (Shuirebaozhaqu) area and Frog Mouth Spring in Tengchong county, Yunnan province, south-western China. The taxonomic positions of the two isolates were investigated by a polyphasic approach. Cells of the two strains were Gram-stain-negative, aerobic and rod-shaped. They were able to grow at 50–70 °C, pH 6.0–8.0 and with a NaCl tolerance up to 0.5 % (w/v). Colonies are circular, convex, non-transparent and produce yellow pigment. Phylogenetic analyses based on 16S rRNA gene sequences comparison clearly demonstrated that strains YIM 77925T and YIM 77777 represent members of the genus 
Thermus
, and they also detected low-level similarities of 16S rRNA gene sequences (below 97 %) compared with all other species in this genus. Their predominant menaquinone was MK-8. The genomic DNA G+C contents of strains YIM 77925T and YIM 77777 were 65.6 mol% and 67.2 mol%, respectively. Based on the results of physiological and biochemical tests and phylogenetic analyses, strains YIM 77925T and YIM 77777 could not be classified as representing any species of the genus 
Thermus
with a validly published name. Thus the two strains are considered to represent a novel species of the genus 
Thermus
, for which the name 
Thermus
caliditerrae sp. nov. is proposed. The type strain is YIM 77925T ( = DSM 25901T = CCTCC 2012061T).

Thermus caliditerrae sp. nov., a novel thermophilic species isolated from a geothermal area

., 2006; Krieg, 2005). All strain are motile, oxidase positive. According to the API 20NE test trips, all are negative for 344 aesculin hydrolysis, all assimilate L-histidine, but not L-arabinose, D-mannitol, adipic acid or phenylacetic acid.+, Positive, utilized; -, negative, not utilized; ND, not 345 determined. 346

1 Key Laboratory of Microbial Diversity in Southwest China, Ministry of

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Thermus caliditerrae sp. nov., a novel thermophilic species isolated from a geothermal area

1 Key Laboratory of Microbial Diversity in Southwest China, Ministry of