Lawrence J. Peck
Harvard University
6 Papers
22 Citations
Lawrence J. Peck is an academic researcher from Harvard University. The author has contributed to research in topics: DNA supercoil & Plasmid. The author has an hindex of 6, co-authored 6 publications.
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Papers
Flipping of cloned d(pCpG)n.d(pCpG)n DNA sequences from right- to left-handed helical structure by salt, Co(III), or negative supercoiling
TL;DR: Negative supercoiling of plasmid DNAs containing 24--42 base pairs of alternating d(pCpG) inserts is shown to cause the flipping of the helical hand of the inserts from right to left under physiological conditions.
319
Energetics of B-to-Z transition in DNA
Lawrence J. Peck,James C. Wang +1 more
TL;DR: The free energy parameters for the transition in dilute aqueous buffers have been evaluated from a statistical mechanical analysis of the data, and these parameters allow prediction of the superhelicities of plasmids at which the transition occurs in alternating C-G inserts over a wide range of lengths.
318
Negatively Supercoiled Plasmids Contain Left-handed Z-DNA Segments As Detected by Specific Antibody Binding
Alfred Nordheim,Eileen M. Lafer,Lawrence J. Peck,James C. Wang,B. David Stollar,Alexander Rich +5 more
TL;DR: The changes in free energy associated with B-Z transitions in superhelical plasmids is calculated on the basis of experimental findings and evidence suggests that the antibody combines with a section of DNA containing 14 bases with alternating purine and pyrimidine residues with one residue out of alternation.
284
Transcriptional block caused by a negative supercoiling induced structural change in an alternating CG sequence
Lawrence J. Peck,James C. Wang +1 more
TL;DR: S1 nuclease mapping of in vivo transcripts from an E. coli gyrase temperature-sensitive mutant harboring the plasmids indicates that the bulk of the transcripts at either permissive or nonpermissive temperatures can proceed through the CG sequence, suggesting that the sequence is normally in the B helical form in vivo.
164
Rate of B to Z structural transition of supercoiled DNA
TL;DR: The forward rate of the B to Z transition induced by negative supercoiling of plasmid DNA containing an alternating C-G sequence has been examined using the binding of Z-DNA-specific antibodies to follow the transition.
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