10 Papers
39 Citations
Kun Wei is an academic researcher from Third Military Medical University. The author has contributed to research in topics: KRAS & Gene. The author has an hindex of 3, co-authored 10 publications.
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Papers
Sensitive and specific detection of miRNA using an isothermal exponential amplification method using fluorescence-labeled LNA/DNA chimera primers.
TL;DR: The ReFIRE system showed high specificity, enabling differentiation between homologous miRNA family members exhibiting only single-base differences, and the sensitivity, specificity, and dynamic range associated with this system were greater than most currently available miRNA isothermal amplification assays.
13
Improved detection of BRAF V600E using allele-specific PCR coupled with external and internal controllers
Zhao Yang,Na Zhao,Dong Chen,Kun Wei,Ning Su,Jun-Fu Huang,Han-Qing Xu,Guangjie Duan,Weiling Fu,Qing Huang +9 more
TL;DR: In this paper, a competitive external allele-specific controller (i.e., rcAS-PCR) was proposed to detect low levels of the mutated BRAF V600E gene.
Concentration and integrity indexes of urine cell-free DNA as promising biomarkers for early lung cancer diagnosis.
Sai Ren,Xiao-Dong Ren,Hai-Qin Guo,Lan Liang,Kun Wei,Li-Fang Guo,Xue-Mei Qu,Xiao-Tian Dai,Qing Huang +8 more
TL;DR: In this article, the role of urine cell-free DNA (ucfDNA) concentration and integrity indexes as potential biomarkers for lung cancer diagnosis was explored, and the results showed that ucfDNA integrity indexes were significantly higher in lung cancer patients than in healthy controls.
10
Enhanced Separation Efficiency and Purity of Circulating Tumor Cells Based on the Combined Effects of Double Sheath Fluids and Inertial Focusing.
Bo-Wen Li,Kun Wei,Qi-Qi Liu,Xian-Ge Sun,Ning Su,Li Wenman,Mei-Yun Shang,Jinmi Li,Dan Liao,Jin Li,Weiping Lu,Shaoli Deng,Qing Huang +12 more
TL;DR: In this article, a double spiral microchannel was developed for efficient separation and enrichment of intact and high-purity CTCs based on the combined effects of two-stage inertial focusing and particle deflection.
Wild‑type blocking pcr coupled with internal competitive amplified fragment improved the detection of rare mutation of KRAS.
Jia Peng,Kun Wei,Xiang Zhao,Ke Yang,Huan Wang,Yang Zhang,Mei Guo,Jing He,Hai-Yan Wu,Li Yongchuan,Na Zhao,Qing Huang,Weiling Fu +12 more
TL;DR: WIRE-PCR facilitated the detection of mutated alleles at a single molecular level and completely blocked the amplification of wild-type KRAS in 50–150 ng DNA templates.