5 Papers
33 Citations
Justyna Nocon is an academic researcher from University of Natural Resources and Life Sciences, Vienna. The author has contributed to research in topics: Pichia pastoris & Flux (metabolism). The author has an hindex of 5, co-authored 5 publications.
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Papers
Pichia pastoris: protein production host and model organism for biomedical research
Brigitte Gasser,Roland Prielhofer,Hans Marx,Michael Maurer,Justyna Nocon,Matthias G. Steiger,Verena Puxbaum,Michael Sauer,Diethard Mattanovich +8 more
TL;DR: Pichia pastoris, as an evolutionarily more 'ancient' yeast, may in some cases be a better model for human cell biology and disease than Saccharomyces cerevisiae.
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Model based engineering of Pichia pastoris central metabolism enhances recombinant protein production
Justyna Nocon,Matthias G. Steiger,Martin Pfeffer,Seung Bum Sohn,Tae Yong Kim,Michael Maurer,Hannes Rußmayer,Stefan Pflügl,Magnus Ask,Christina Haberhauer-Troyer,Karin Ortmayr,Stephan Hann,Gunda Koellensperger,Brigitte Gasser,Sang Yup Lee,Diethard Mattanovich +15 more
TL;DR: Genome scale metabolic modeling is shown to predict overexpression and deletion mutants which enhance recombinant protein production with high accuracy and has a strong impact on intracellular fluxes.
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Increasing pentose phosphate pathway flux enhances recombinant protein production in Pichia pastoris
Justyna Nocon,Matthias G. Steiger,Teresa Mairinger,Jonas Hohlweg,Hannes Rußmayer,Stephan Hann,Brigitte Gasser,Diethard Mattanovich +7 more
TL;DR: It is shown that overexpression of SOL3 together with ZWF1 enhanced both the PPP flux ratio and hSOD accumulation, providing evidence that in P. pastoris Sol3 limits the flux through PPP and recombinant protein production.
Gas Chromatography-Quadrupole Time-of-Flight Mass Spectrometry-Based Determination of Isotopologue and Tandem Mass Isotopomer Fractions of Primary Metabolites for (13)C-Metabolic Flux Analysis.
Teresa Mairinger,Matthias G. Steiger,Justyna Nocon,Diethard Mattanovich,Gunda Koellensperger,Stephan Hann +5 more
TL;DR: The method provides a powerful new toolbox for (13)C-based metabolic flux analysis, which is an emerging strategy in metabolic engineering and outperforms previous approaches in terms of metabolite coverage, while also providing rich isotopomer information for a significant number of key metabolites.
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Sample preparation workflow for the liquid chromatography tandem mass spectrometry based analysis of nicotinamide adenine dinucleotide phosphate cofactors in yeast
Karin Ortmayr,Justyna Nocon,Brigitte Gasser,Diethard Mattanovich,Stephan Hann,Gunda Koellensperger +5 more
TL;DR: A thorough evaluation and optimization of the sample preparation procedure resulted in excellent biological repeatabilities without employing an internal standardization approach, and the methodology proved to be appropriate for the relative assessment of intracellular levels of oxidized and reduced nicotinamide adenine dinucleotide phosphate in different P. pastoris strains.