. Inrenaltransplantpatients,immunosuppressivetherapymayresultinthereactivationofpolyomavirusBK(BKV),leadingtopolyomavirus-associatednephropathy(PVAN),whichinevitablycausesallograftfailure.Sincethetreatment outcomes of PVAN remain unsatisfactory, early identification and continuous monitoring of BKV reac-tivationandreductionof immunosuppressantsareessential topreventPVANdevelopment. Thepresent studydem-onstrated that the developed dual-channel heterodyne-based surface plasmon resonance (SPR) biosensor isapplicable for the rapid detection of urinary BKV. The use of a symmetrical reference channel integrated withthe poly(ethylene glycol)-based low-fouling self-assembled monolayer to reduce the environmental variationsand the nonspecific noise was proven to enhance the sensitivity in urinary BKV detection. Experimentally, thedetection limit of the biosensor for BKV detection was estimated to be around 8500 copies∕mL. In addition,urine samples from five renal transplant patients were tested to rapidly distinguish PVAN-positive and PVAN-neg-ative renal transplant patients. By virtue of its simplicity, rapidity, and applicability, the SPR biosensor is a remark-able potential to be used for continuous clinical monitoring of BKV reactivation.

https://www.spiedigitallibrary.org/journals/Journal-of-Biomedical-Optics/volume-19/issue-01/011013/Rapid-detection-of-urinary-polyomavirus-BK-by-heterodyne-based-surface/10.1117/1.JBO.19.1.011013.pdf

Rapid detection of urinary polyomavirus BK by heterodyne-based surface plasmon resonance biosensor.

Real-time SPR characterization of the interactions between multi-epitope proteins and antibodies against classical swine fever virus.

Vascular endothelial growth factor(VEGF) is a vital mediator of angiogenesis.VEGF receptor 2(VEGFR2) interacts with VEGF,as one of its specific membrane receptors.Researchers have found out that a VEGF blocker can restrain some vital processes of tumor suppression by blocking the signal pathway of VEGF-VEGFR2.However,there is no literature evidence of investigation on the antiangiogenic ability of single domain 3 of VEGFR-2(KDR3),as the key domain in the signal transduction of VEGF.In this article,Escherichia coli Rosetta-gami(DE3)/pET22b was taken to express the soluble KDR3.The binding between KDR3 and VEGF165 was tested with BiacoreX100(GE).Scratch-wound healing assay was used to evaluate KDR3’s cell migration ability.The in vivo anti-angiogenic effect of KDR3 was tested with chicken embryo chorioallantoic membrane(CAM) model.The outcome showed that the soluble KDR3 was expressed successfully with prokaryotic expression system.The surface plasmon resonance(SPR) technology was adopted to test the binding between KDR3 and VEGF165,which equilibrium dissociation rate constant(Kd) was 54 nmol/L.KDR3 can suppress EA.hy926 cell’ migration with a dose-dependent tendency.Also the in vivo investigation showed that KDR3 is capable of inhibiting neovascularization,adopting the CAM assay.This article has therefore provided a novel antiangiogenic drug candidate relating to VEGF-VEGFR2 pathway.

Antiangiogenic Activity Research of the Vascular Endothelial Growth Factor Receptor 2 Extracellular Region 3 (KDR3)

Real-time analysis of the interaction of a multiple-epitope peptide with antibodies against classical swine fever virus using surface plasmon resonance.

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Real-time analysis of the interaction of a multiple-epitope peptide with antibodies against classical swine fever virus using surface plasmon resonance.