Several methods to determine amino acids and vitamins in biological and pharmaceutical samples have been reported Thin layer chromatography (TLC) finds its place when the relatively costly equipment required by other methods is unavailable This review covers the 1991–2010 literature on TLC/HPTLC (high performance thin layer chromatography) amino acid and vitamin determinations It gives an overview of the special features as well as the problems in TLC/HPTLC determinations of amino acids and vitamins Various chromatographic systems useful in amino acid and vitamin identification, separation and quantitation of are presented in tabular form Future prospects of TLC/HPTLC for amino acid and vitamin determinations are also discussed 
Open image in new window

/pdf/amino-acid-and-vitamin-determinations-by-tlc-hptlc-review-of-2flfmw83bp.pdf

Amino acid and vitamin determinations by TLC/HPTLC: review of the current state

Determination of protein structure is very dependent on amino acid detection, because these compounds form the backbones of proteins, and also for determination of the C-terminal units of degraded proteins. Several selective and nonselective reagents have been used for detection and identification of amino acids on thin-layer chromatograms [1–20]. Among the reagents used, ninhydrin is the most well-known nonselective reagent. It is widely used, because of its high sensitivity [2], but produces same color, purple–violet, for all amino acids except proline and hydroxyproline (yellow). To overcome this problem 2,3dichloro-1,4-naphthaquinone and isatin have been introduced as a new reagent which produces distinguishable and stable colors (more or less stable for 24 to 48 h) with many amino acids and enables convenient and easy detection of these compounds on silica gel G TLC plates with very high sensitivity (detection limit between 0.01 and 0.3 μg).

New reagent for detection of amino acids on TLC plates

Thin-layer chromatography (TLC) of fifteen amino acids was performed using silica gel and alumina impregnated with micellar solutions of cetrimide and cetylpyridinium chloride as stationary phases and aqueous solutions of dextrose as mobile phases. TLC system comprising of silica gel impregnated with micellar solution of cetrimide (5.0 mM) as stationary phase and 40% ( w/v ) aqueous solution of dextrose as mobile phase was found the most favourable for the separation of amino acids. Impregnation of silica gel with the micellar solution of cetrimide brings about a substantial change in the mobility of lysine. Separation of lysine (ketogenic) from arginine (glucogenic) is important physiologically. Surface modification of silica gel on impregnation, as indicated by FTIR and SEM studies, was responsible for improved chromatographic performance. The effect on the separation of the presence in the sample of heavy metal cations, as impurities, was examined. Limits of detection for lysine and arginine were 0.17 ...

TLC separation of amino acids with a green mobile phase

A new green TLC has been used for identifying and monitoring the migration behavior of amino acids through silica and kieselguhr static flat bed in contact of n-butyl alcohol, ethyl acetate or ethylene glycol and their mixtures. From the point of view of chromatographic performance, a mixture of n-butyl alcohol-70% aqueous ethylene glycol-ethyl acetate ratio 5:3:2 by volume proves to be more efficient than the individual components for separation of amino acids from their binary, ternary and quaternary mixtures and the chromatographic parameters like ΔR(F) , separation factor (α) and resolution (R(S) ) for the separation were calculated. Effect of the presence of foreign substances such as metal cations, anions, vitamins and pesticides as impurities in the sample on the separation was also examined. Effect of substitution of butanol by various alcohols has been examined to assess the impact of hydrophobicity of alcohols on the separation of amino acids. The limits of detection for tyrosine, tryptophan, alanine, isoleucine, methionine and serine were found to be 0.10 μg/spot, whereas for lysine it is 0.05 μg/spot. Application of the selected TLC system for the identification and separation of amino acids present in drugs/pharmaceuticals has been performed.

Resolution of multicomponent mixture of amino acids using environmentally benign eluents: A green chromatographic approach

Surfactants modified silica phase for sorption studies of essential amino acids by thin layer chromatography

A variety of selective and non-selective reagents [1–18] are used in thin-layer chromatography for identification of amino acids. Such identification is very useful because of the occurrence of the amino acids as the structural units of proteins, in the free state in natural products, and for determination of the C-terminal amino acids of degraded proteins. Of the reagents generally used, ninhydrin, a non-selective reagent, is popular because of its remarkably high sensitivity [2], even though it produces the same purple color with all the amino acids except proline and hydroxyproline. To solve this problem a new reagent, 4hydroxyacetophenone–isatin-5-sulfonic acid (sodium salt) is proposed. This communication deals with this reagent, which produces distinguishable colors for most of the amino acids. With the proposed reagent detection limits for the amino acids range between 0.1 and 2.0 μg.

A new reagent for identification of amino acids on thin-layer chromatography plates

Popular Tasks used by Researchers

How can I help with your research?

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

We encountered an error while loading the panel. Please try refreshing the page.

Something went wrong

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

Anyone with the link can view this chat. You can switch it back to private anytime.

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

Dipsikha Panja

Author Tools

Chat about Author

Papers

A new reagent for identification of amino acids on thin-layer chromatography plates