An apparatus includes a substrate, a first heating element, and a second heating element. The substrate includes a first portion, a second portion, and a third portion that is between the first portion and the second portion. The first portion is characterized by a first thermal conductivity, the second portion is characterized by a second thermal conductivity, and the third portion is characterized by a third thermal conductivity. The third thermal conductivity is less than the first thermal conductivity and the second thermal conductivity. The first heating element is coupled to the first portion of the substrate, and is configured to produce a first thermal output. The second heating element is coupled to the second portion of the substrate, and configured to produce a second thermal output. The second thermal output is different from the first thermal output.

Printed circuit board heater for an amplification module

The present invention relates to a communicator, a combination of the communicator and a reagent module, and a microfluidic chip. The communicator comprises a needle plate and at least a communicationassembly, wherein the communication assembly comprises a first channel arranged in the needle plate, a first needle head connected to the needle plate and communicated with the first channel, and a second needle head connected to the needle plate and communicated with the first channel, and the first channel is used for guiding a liquid introduced by the first needle head to the second needle head. According to the invention, the communicator has piercing and communicating functions, can guide a liquid, can be suitable for different reagent modules by changing the arrangement of channels andneedle heads, is beneficial to enabling reagent storage modules to be independent, and enables the reagent storage bin of an independent reagent storage chip to be communicated with the reagent reaction bin of a reagent reaction module during an experiment.

Communicator, combination of communicator and reagent module, and microfluidic chip

An infection detection system includes a sampling device, a lysing chamber, a filter, a meter, a NASBA fluidic network, and an instrument. The sampling device is configured to contain a whole blood sample containing a pathogen target. The lysing chamber is configured to be in fluid communication with the sampling device to receive the whole blood sample. The lysing chamber is configured to lyse the whole blood sample into a lysate. The filter is configured to be in fluid communication with the lysing chamber and to filter the lysate into a filter lysate. The meter is configured to be in fluid communication with the filter and configured to meter a predetermined amount of filtered lysate from the filtered lysate. The NASBA fluidic network is configured to be in fluid communication with the meter to receive the predetermined amount of filtered lysate.

Infection detection systems and methods including a sample processor having integrated sample filter and meter

The invention discloses a use of Aliivibrio fischeri strain DNA. The Aliivibrio fischeri strain DNA is used for determining a DNA extraction rate of a soil sample. The invention also discloses a method for determining a DNA extraction rate of a soil sample. The method comprises the following steps: culturing the Aliivibrio fischeri ATCC 7744T strain, extracting DNA of the Aliivibrio fischeri ATCC7744T strain, and using as an internal standard for determining the DNA extraction rate of the soil sample; designing a specific primer of a 16S rRNA gene fragment in Aliivibrio fischeri; adding DNA of the internal standard in the soil sample, and then immediately extracting the DNA; determining the gene quantity of the internal standard added into the soil and the gene quantity of the internal standard in the soil sample after the extraction by adopting qPCR, and calculating the DNA extraction rate.

Method for determining DNA extraction rate of soil sample

An infection detection system includes a sampling device, a meter, a sample processor, and an analytical instrument. The sampling device is configured to collect a whole blood sample containing a pathogen target. The meter is configured to be removably connected to a portion of the sampling device to meter a predetermined amount of the whole blood sample. The sample processor includes a NASBA fluidic network configured to be in fluid communication with the meter to receive the whole blood sample. The NASBA fluidic network includes an enzyme, a primer, and a beacon. The enzyme and a primer are for amplifying a predetermined genetic sequence in the pathogen target. The beacon is configured to attach to the predetermined genetic sequence in the pathogen target.

Infection detection systems and methods including intermediate filtering and metering

A method includes conveying a detection solution containing a target amplicon into a detection module of a molecular diagnostic test device. The detection module includes a detection surface including a series of capture probes to which a first portion of the target amplicon is bound when the detection solution is conveyed. A first reagent formulated to produce a visible signal indicating a presence of the target amplicon is then conveyed into the detection module. The first reagent is bound to a second portion of the target amplicon when the first reagent is conveyed. A second reagent is conveyed into the detection module. The second reagent includes a precipitating substrate formulated to catalyze the production of the visible signal by producing an insoluble colored product when the second reagent is in contact with the first reagent. The method includes viewing the visible signal via a transparent portion of the detection module.

Devices and methods for the detection of molecules using a flow cell

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Devices and methods for the detection of molecules using a flow cell