Thiazolidinediones (TZDs) are widely used to treat type 2 diabetes mellitus; however, their use is complicated by systemic fluid retention. Along the nephron, the pharmacological target of TZDs, peroxisome proliferator-activated receptor-gamma (PPARgamma, encoded by Pparg), is most abundant in the collecting duct. Here we show that mice treated with TZDs experience early weight gain from increased total body water. Weight gain was blocked by the collecting duct-specific diuretic amiloride and was also prevented by deletion of Pparg from the collecting duct, using Pparg (flox/flox) mice. Deletion of collecting duct Pparg decreased renal Na(+) avidity and increased plasma aldosterone. Treating cultured collecting ducts with TZDs increased amiloride-sensitive Na(+) absorption and Scnn1g mRNA (encoding the epithelial Na(+) channel ENaCgamma) expression through a PPARgamma-dependent pathway. These studies identify Scnn1g as a PPARgamma target gene in the collecting duct. Activation of this pathway mediates fluid retention associated with TZDs, and suggests amiloride might provide a specific therapy.

Thiazolidinediones expand body fluid volume through PPARγ stimulation of ENaC-mediated renal salt absorption

https://www.cell.com/cell-metabolism/pdf/S1550-4131(12)00498-6.pdf

Genetic Control of Obesity and Gut Microbiota Composition in Response to High-Fat, High-Sucrose Diet in Mice

Author(s): Jurgens, Hella; Haass, Wiltrud; Castaneda, Tamara R; Schurmann, Annette; Koebnick, Corinna; Dombrowski, Frank; Otto, Barbel; Nawrocki, Andrea R; Scherer, Philipp E; Spranger, Jochen; Ristow, Michael; Joost, Hans-Georg; Havel, Peter J; Tschop, Matthias H | Abstract: ObjectiveThe marked increase in the prevalence of obesity in the United States has recently been attributed to the increased fructose consumption. To determine if and how fructose might promote obesity in an animal model, we measured body composition, energy intake, energy expenditure, substrate oxidation, and several endocrine parameters related to energy homeostasis in mice consuming fructose.Research methods and proceduresWe compared the effects of ad libitum access to fructose (15% solution in water), sucrose (10%, popular soft drink), and artificial sweetener (0% calories, popular diet soft drink) on adipogenesis and energy metabolism in mice.ResultsExposure to fructose water increased adiposity, whereas increased fat mass after consumption of soft drinks or diet soft drinks did not reach statistical significance (n = 9 each group). Total intake of energy was unaltered, because mice proportionally reduced their caloric intake from chow. There was a trend toward reduced energy expenditure and increased respiratory quotient, albeit not significant, in the fructose group. Furthermore, fructose produced a hepatic lipid accumulation with a characteristic pericentral pattern.DiscussionThese data are compatible with the conclusion that a high intake of fructose selectively enhances adipogenesis, possibly through a shift of substrate use to lipogenesis.

/pdf/consuming-fructose-sweetened-beverages-increases-body-4bcnu41uz9.pdf

Consuming fructose-sweetened beverages increases body adiposity in mice

Reliable and valid body composition assessment is important in both clinical and research settings. A multitude of methods and techniques for body composition measurement exist, all with inherent problems, whether in measurement methodology or in the assumptions upon which they are based. This review is focused on currently applied methods for in vivo measurement of body composition, including densitometry, bioimpedance analysis, dual-energy X-ray absorptiometry, computed tomography (CT), magnetic resonance techniques and anthropometry. Multicompartment models including quantification of trace elements by in vivo neutron activation analysis, which are regarded as gold standard methods, are also summarized. The choice of a specific method or combination of methods for a particular study depends on various considerations including accuracy, precision, subject acceptability, convenience, cost and radiation exposure. The relative advantages and disadvantages of each method are discussed with these considerations in mind.

Contemporary methods of body composition measurement.

The present invention relates to methods and devices for analyzing x-ray images. In particular, devices, methods and algorithms are provided that allow for the accurate and reliable evaluation of bone mineral density and bone structure from x-ray images.

Methods and devices for analysis of x-ray images

Objective: to evaluate the applicability, precision, and accuracy of the new EchoMRI quantitative magnetic resonance (QMR) method for in-vitro bovine bone analysis and in-vivo whole-body-composition analysis of conscious live mice. Research methods and procedures: bovine tibia bone samples were measured by QMR and dual-energy X-ray adsorptiometry (DEXA). Repeated measures of whole-body composition were made using live and dead mice with different levels of fat by QMR and DEXA and by classic chemical analysis of the mouse carcass. Results: bone-mineral density (BMD) and bone-mineral content (BMC) measured in bovine tibia by QMR and DEXA were highly correlated. Precision of fat and lean measurement in mice was found to be better for QMR than for DEXA. The coefficient of variation (CV) for fat was 0.34–0.71% for QMR compared with 3.06–12.60% for DEXA. Discussion: QMR offers more specific parameters of bone structure than does DEXA. QMR and DEXA did not differ in the total amount of fat detected in live mice but QMR had improved precision. QMR was superior to DEXA in measuring fat in very small mice. Conclusions: in bone tissue there is a strong correlation between hydrogen NMR signal and bone-mineral density as measured by X-ray. QMR provides a very precise, accurate, fast, and easy to use method for determining fat and lean mass of mice without the need for anesthesia. Its ability to detect differences and monitor changes in body composition in mice with great precision should be of great value in characterizing phenotypes and studying drugs affecting obesity.

Quantitative magnetic resonance (QMR) method for bone and whole-body-composition analysis

A nuclear magnetic resonance sensing apparatus including a magnet for inducing a static magnetic field in materials to be analyzed, the magnetic field having uniform field strength around, being substantially rotationally symmetric and perpendicular to a longitudinal axis of the magnet. The apparatus includes means for generating a radio frequency magnetic field in the materials to be analyzed. The radio frequency magnetic field is substantially rotationally symmetric about the longitudinal axis and is parallel to the longitudinal axis within a sensitive volume containing the materials to be analyzed. The apparatus includes means for receiving a nuclear magnetic resonance signal from the sensitive volume. In a preferred embodiment, the means for generating and means for receiving include an antenna coil wound so that turns of the coil lie in planes perpendicular to the longitudinal axis of the magnet.

Nuclear magnetic resonance well logging apparatus and method adapted for measurement-while-drilling

A combined electromagnetic acoustic transducer (EMAT) is disclosed adapted to generate both SH-type acoustic waves and LAMB-type acoustic waves in a conductive casing, surroundings of which are to be analyzed. The transducer comprises one magnet assembly and two RF coils implemented as multi-layer printed circuit board. Each coil is used to generate or receive acoustic signals of one wave type. Compared to using two single-wave-type transducers the combined EMAT significantly reduces total attraction force to the casing and, correspondingly, simplifies mechanics of the measurement tool. It is emphasized that this abstract is provided to comply with the rules requiring an abstract which will allow a searcher or other reader to quickly ascertain the subject matter of the technical disclosure. It is submitted with the understanding that it will not be used to interpret or limit the scope or meaning of the claims.

Combined Electro-Magnetic Acoustic Transducer

A side-looking NMR logging tool incorporates a permanent magnet arrangement (1) having a magnetization direction oriented towards a side of the tool and a dipole RF antenna (10) displaced towards the front of the tool. The magnetic arrangement produces a shaped region of investigation in front of the tool wherein the magnetic field has a uniform field strength and the RF field has a uniform field strength in a direction orthogonal to the static field. The shaping of the static field is accomplished by the magnet arrangement comprising a plurality of magnets (8) having parallel magnetization or by a single shaped magnet (8). The antenna arrangement includes gapped core made of non-ferritic soft material for increasing antenna efficiency. The magnet arrangement also reduces ringing in the core and the antenna. An optional RF shield is used to reduce NMR signals from borehole fluids.

Side-looking nmr probe for oil well logging

A method for determining nuclear magnetic resonance longitudinal relaxation time of a medium. The method includes magnetically polarizing nuclei in the medium along a static magnetic field, momentarily inverting the magnetic polarization of the nuclei within each one of a plurality of different spatial volumes within the medium, transversely magnetizing the nuclei in each one of the spatial volumes after an individual recovery time corresponding to each one of the spatial volumes, and measuring an amplitude of a magnetic resonance signal from each one of the spatial volumes.

Multiple frequency method for nuclear magnetic resonance longitudinal relaxation measurement and pulsing sequence for power use optimization

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