Abdul Bhuiya
University of California, Berkeley
10 Papers
19 Citations
Abdul Bhuiya is an academic researcher from University of California, Berkeley. The author has contributed to research in topics: RNA & Biology. The author has an hindex of 6, co-authored 9 publications. Previous affiliations of Abdul Bhuiya include University of California, San Francisco & University of Illinois at Urbana–Champaign.
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Papers
Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy.
Parinaz Fozouni,Sungmin Son,María Díaz de León Derby,Gavin J. Knott,Gavin J. Knott,Carley N. Gray,Carley N. Gray,Michael V. D’Ambrosio,Chunyu Zhao,Neil A. Switz,G. Renuka Kumar,G. Renuka Kumar,Stephanie I. Stephens,Stephanie I. Stephens,Daniela Boehm,Daniela Boehm,Chia-Lin Tsou,Chia-Lin Tsou,Jeffrey Shu,Jeffrey Shu,Abdul Bhuiya,Maxim Armstrong,Andrew R. Harris,Pei Yi Chen,Pei Yi Chen,Jeannette M. Osterloh,Anke Meyer-Franke,Bastian Joehnk,Keith Walcott,Anita Sil,Charles Langelier,Katherine S. Pollard,Emily D. Crawford,Andreas S. Puschnik,Maira Phelps,Amy Kistler,Joseph L. DeRisi,Jennifer A. Doudna,Daniel A. Fletcher,Melanie Ott +39 more
TL;DR: This work reports the development of an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2 from nasal swab RNA that can be read with a mobile phone microscope and has the potential to enable rapid, low-cost, point-of-care screening for Sars-Cov-2.
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Accelerated RNA detection using tandem CRISPR nucleases.
Tina Y. Liu,Gavin J. Knott,Gavin J. Knott,Dylan C. J. Smock,John J Desmarais,Sungmin Son,Abdul Bhuiya,Shrutee Jakhanwal,Noam Prywes,Shreeya Agrawal,María Díaz de León Derby,Neil A. Switz,Maxim Armstrong,Andrew R. Harris,Emeric J Charles,Brittney W. Thornton,Parinaz Fozouni,Jeffrey Shu,Jeffrey Shu,Stephanie I. Stephens,Stephanie I. Stephens,G. Renuka Kumar,G. Renuka Kumar,Chunyu Zhao,Amanda Mok,Anthony T. Iavarone,Arturo M. Escajeda,Roger McIntosh,Shineui Kim,Eli J Dugan,Katherine S. Pollard,Katherine S. Pollard,Ming X. Tan,Melanie Ott,Melanie Ott,Daniel A. Fletcher,Liana F. Lareau,Patrick D. Hsu,David F. Savage,Jennifer A. Doudna +39 more
TL;DR: Fast Integrated Nuclease Detection In Tandem (FIND-IT) as mentioned in this paper combines RNA-guided Cas13 and Csm6 with a chemically stabilized activator to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA extracted from respiratory swab samples with quantitative reverse transcriptase PCR (qRT-PCR)-derived cycle threshold values up to 33.
Hands-free smartphone-based diagnostics for simultaneous detection of Zika, Chikungunya, and Dengue at point-of-care.
Anurup Ganguli,Akid Ornob,Hojeong Yu,Gregory L. Damhorst,Weili Chen,Fu Sun,Abdul Bhuiya,Brian T. Cunningham,Rashid Bashir +8 more
TL;DR: A novel point-of-care platform to diagnose infectious diseases from whole blood samples using a commercial smartphone to acquire real-time images of the amplification reaction and displays a visual read-out of the assay.
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Rapid detection of SARS-CoV-2 RNA in saliva via Cas13
Sita S. Chandrasekaran,Shreeya Agrawal,Alison Fanton,Aditya Jangid,Bérénice Charrez,A. M. Escajeda,S. Son,Roger McIntosh,Huyen Tran,Abdul Bhuiya,Maria Diaz de Leon Derby,Neil A. Switz,Maxim Armstrong,Andrew R. Harris,Noam Prywes,Maria Lukarska,Scott B. Biering,Dylan C. J. Smock,Amanda Mok,Gavin J. Knott,Qi Dang,Erik Van Dis,Eli J Dugan,Shin Kim,Tina Y. Liu,Jennifer Hamilton,Enrique Lin-Shiao,Elizabeth C. Stahl,Connor A. Tsuchida,Petros Giannikopoulos,Matthew McElroy,Shana L. McDevitt,Arielle Zur,Iman Sylvain,Alison Ciling,Madeleine Zhu,Clara Williams,Alisha Baldwin,Erica A. Moehle,Katherine Kogut,Brenda Eskenazi,Eva Harris,Sarah A. Stanley,Liana F. Lareau,Ming X. Tan,Daniel A. Fletcher,Jennifer A. Doudna,David F. Savage,Patrick D. Hsu +48 more
TL;DR: In this paper , the authors reported an assay for rapid COVID-19 testing and its implementation in a prototype microfluidic device called DISCoVER (for diagnostics with coronavirus enzymatic reporting), which involves extraction-free sample lysis via shelfstable and low-cost reagents, multiplexed isothermal RNA amplification followed by T7 transcription, and Cas13-mediated cleavage of a quenched fluorophore.
Accelerated RNA detection using tandem CRISPR nucleases
Tina Y. Liu,Gavin J. Knott,Gavin J. Knott,Dylan C. J. Smock,John J Desmarais,Sungmin Son,Abdul Bhuiya,Abdul Bhuiya,Shrutee Jakhanwal,Noam Prywes,Shreeya Agrawal,María Díaz de León Derby,María Díaz de León Derby,Neil A. Switz,Maxim Armstrong,Andrew R. Harris,Emeric J Charles,Brittney W. Thornton,Parinaz Fozouni,Jeffrey Shu,Jeffrey Shu,Stephanie I. Stephens,Stephanie I. Stephens,G. Renuka Kumar,G. Renuka Kumar,Chunyu Zhao,Amanda Mok,Anthony T. Iavarone,Arturo M. Escajeda,Roger McIntosh,Shineui Kim,Eli J Dugan,Katherine S. Pollard,Katherine S. Pollard,Ming X. Tan,Melanie Ott,Melanie Ott,Daniel A. Fletcher,Daniel A. Fletcher,Liana F. Lareau,Patrick D. Hsu,David F. Savage,Jennifer A. Doudna +42 more
TL;DR: Fast Integrated Nuclease Detection In Tandem (FIND-IT) as mentioned in this paper was proposed to detect SARS-CoV-2 RNA from nasopharyngeal samples with PCR-derived Ct values up to 29 in microfluidic chips.