TL;DR: Two new benzo[c]phenanthridine alkaloids and 6-methyldihydrochelerythrine, together with 23 known compounds, were isolated from the root bark of Zanthoxylum simulans and exhibited cytotoxic activity.
Abstract: Two new benzo[c]phenanthridine alkaloids, 6-methyldihydrochelerythrine [1] and 6-methylnorchelerythrine [2], together with 23 known compounds, were isolated from the root bark of Zanthoxylum simulans. Structures were elucidated by spectral analysis. Among them, the pyranoquinoline alkaloids, zanthosimuline [3], and huajiaosimuline [4], exhibited cytotoxic activity. In addition, compound 4 showed significant antiplatelet aggregation activity and induced terminal differentiation with cultured HL-60 cells.
TL;DR: Three new pyranoquinoline alkaloids, simulenoline, peroxysimulenoline and benzosimuline, together with 22 known compounds, were isolated from the stem bark of Formosan Zanthoxylum simulans, showing strong anti-platelet aggregation activity in vitro.
TL;DR: Among the isolates, 11 compounds showed anti-platelet aggregation activity in vitro and their structures were determined through spectral analyses.
TL;DR: Three new 2-quinolinone alkaloids are isolated and characterized from the root bark of Zanthoxylum simulans, and Simulanoquinoline is the first naturally occurring dimeric alkaloid between dihydrobenzo[c]phenanthridine and2-quinolone joined by a C-C linkage.
TL;DR: Five endospore-forming, nitrogen-fixing strains isolated from rhizosphere soils of Zanthoxylum simulans planted in Beijing, China revealed that the five strains formed a distinct cluster within the genus Paenibacillus, for which the name PaenIBacillus zanthoxyli sp.
Abstract: Five endospore-forming, nitrogen-fixing strains were isolated from rhizosphere soils of Zanthoxylum simulans planted in Beijing, China. Phylogenetic analysis based on full-length 16S rRNA gene sequences revealed that the five strains formed a distinct cluster within the genus Paenibacillus. High levels of 16S rRNA gene sequence similarity were found between these novel strains and Paenibacillus azotofixans ATCC 35681(T) (97.8-98.5 % similarity) and Paenibacillus stellifer DSM 14472(T) (95.4-96.3 %). Levels of 16S rRNA gene sequence similarity between the novel isolates and other species of the genus Paenibacillus were less than 95.0 %. Levels of 16S rRNA gene sequence similarity among the isolates were more than 98.0 %. DNA-DNA relatedness between the five novel isolates and P. azotofixans ATCC 35681(T) was 45.50-47.45 % and relatedness among the five novel strains was 95.8-99.6 %. A significant feature of the novel strains that differentiated them from P. azotofixans and other Paenibacillus species was that none of the novel strains could produce acid or gas from the following various carbohydrates: glucose, sucrose, lactose, fructose, glycerol, xylose, maltose, d-sorbitol, sodium succinate, sodium citrate, glycine or l-aspartate. Anteiso-branched C(15 : 0) was the major fatty acid component (36.59 %) of novel strain JH29(T). On the basis of phenotypic properties, 16S rRNA gene sequences, DNA G+C content, DNA-DNA hybridization, chemotaxonomic properties and the nifH gene sequence, the five novel strains form a very homogeneous group which is different from other related species within the genus Paenibacillus. Therefore, the five novel strains are considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus zanthoxyli sp. nov. is proposed. The type strain is JH29(T) (=CCBAU 10243(T)=DSM 18202(T)).