TL;DR: A high-performance liquid chromatography method for the determination of urea that incorporates automated derivatisation with xanthydrol (9H-xanthen-9-ol) is described and the limit of detection for urea was 5 x 10(-8) M (0.003 mg L(-1)).
TL;DR: NA cells of the adrenal medulla, another kind of cell in the carotid body and chemodectomas, as well as ruminants' mast cells, reacted like catecholamines in in vitro tests.
Abstract: Factors influencing the fixation of biogenic amines by solutions of glutaraldehyde, formaldehyde, acrolein and glyoxal as well as the influences of these aldehydes on the histochemical reactivity of biogenic amines, have been extensively investigated. Selective stainings of 5-hydroxytryptamine, noradrenaline, dopamine and related substances in many aldehyde-fixed tissues have been achieved by systematically applying some “phenol” methods such as argentaffin, ferric-ferricyanide, diazonium and thioindoxyl reactions, and some “indole” methods such as xanthydrol, dimethylaminobenzaldehyde, vanilline, salicilaldehyde and benzaldehyde tests.
TL;DR: The facility of the histochemical technique as well as its specificity, sensitivity, and sharp localizability recommends the use of the rosindole reaction in routine histopathological investigation.
Abstract: 1. The application of the rosindole reaction of E. Fischer to the histochemical localization of indole derivatives is described. This method demonstrates predominantly the tryptophan moiety of proteins and it therefore depends on the degree of protein fixation produced by formaldehyde.2. The specificity of the rosindole reaction is established and the comparison is made between it and both the xanthydrol and post-coupled benzylidene reactions.3. The facility of the histochemical technique as well as its specificity, sensitivity, and sharp localizability recommends the use of the rosindole reaction in routine histopathological investigation.
TL;DR: In this work, three different phages displayed peptides with capability of identifying the xanthyl ethyl carbamate immunocomplex were isolated from phage library and the binding mechanism of peptides and Immunocomplex was studied by computer-assisted simulation, resulting in a sensitive non-competitive immunoassay.