Topic
Wright Staining Method
About: Wright Staining Method is a research topic. Over the lifetime, 2 publications have been published within this topic receiving 7 citations.
Papers
Patent•
25 Sep 2013
TL;DR: In this article, a liquid-based cell analyzer staining method for cells exfoliated from serous cavity by using Wright Staining method, and relates to the cell staining methods for the cells exporters from serrous cavity of a liquid based cell system.
Abstract: The invention discloses a liquid-based cell analyzer staining method for cells exfoliated from serous cavity by using Wright staining method, and relates to the cell staining method for the cells exfoliated from serous cavity of a liquid-based cell system. The method comprises following steps: (1) preparing different systematic dye solutions, and preparing buffer solutions with a pH gradient; (2) performing matched experiments of different concentrations by simulation of analyzer procedures, observing effects of processes by using a microscope, selecting optimum staining concentrations of reagents by using an optimum seeking method, and then performing the analyzer procedures until the effects are consistent with conventional effects; (3) optimally selecting a best staining system as the Wright staining solution, and setting staining operation parameters of slice-making of the liquid-based cell analyzer; (3) slice-making settling time is 8 to 12 min, staining time of the Wright staining solution and the buffer solutions is 8 to 12 min, and washing time of the buffer solutions is 2 to 4 times. Characteristics of the cell staining method are that: cost is lower, cell shading effect is excellent, cell volumes are basically unchanged, the color of cytolymph and the color of cell nucleus are obviously different from each other, problems of conventional staining method of the analyzer are solved, and positive diagnosis rate is increased.
5 citations
Patent•
22 Dec 2017
TL;DR: In this article, a blood cell staining method consisting of three steps is presented. The method comprises the steps as follows: preparation of a staining solution, preparation of staining buffer solution, staining and the like.
Abstract: The invention belongs to the technical field of blood cell staining and discloses a blood cell staining method. The method comprises the steps as follows: preparation of a staining solution, preparation of a staining buffer solution, staining and the like, wherein each liter of the staining buffer solution is prepared from 0.3 g of potassium dihydrogen phosphate, 0.2 g of disodium hydrogen phosphate, 5-10 ml of glycerol and 5-10 mu L of hydrogen peroxide with the mass fraction of 30% through mixing, and distilled water is added to the constant volume of 1L. According to the method, the staining buffer solution used in a Wright staining method is improved, hydrogen peroxide is added on the basis of the traditional buffer solution, production of azure is promoted under the action of oxidation of the hydrogen peroxide, so that after the prepared staining buffer solution and the staining solution are mixed, all that is required is to place an obtained mixture for 5-10 min, the mixture can be used, the staining effect is not affected, the staining solution only needs to be placed for 3 d after being prepared and can be used in cooperation with the staining buffer solution, and consumption time is short.
2 citations
Performance Metrics
| Year | Papers |
|---|---|
| 2017 | 1 |
| 2013 | 1 |