TL;DR: The continuous increase in branched-chain fatty acids suggested an increase in gram-positive bacteria and actinomycetes, and the decrease in polyunsaturated fatty acids indicated a decrease in eukaryotic cells during the composting.
Abstract: Determination of different indicators of microbial biomass, community structure, and bioactivity by the fumigation extraction method, as well as determination of phospholipid fatty acids (PLFAs) and their subfractions and the measurement of trace gases, respectively, provides valuable information about microbial succession in composting processes. The emission rates of carbon dioxide (CO(inf2)), methane (CH(inf4)), and nitrous oxide (N(inf2)O) increased successively during compost maturation: initially in the presence of easily degradable nutrients, during high temperature, and after the temperature had cooled down, respectively. The emission rate patterns of these trace gases corresponded to the concentrations of PLFAs and their particular subfractions. (i) Similar to the CO(inf2) emission rates, microbial biomass estimations by fumigation extraction and by determination of the amount of total PLFAs showed a discontinuous decrease during the composting process, with a slight increase at the end of the observation period. (ii) An increase in ether lipids, indicating the enhanced presence of archaean methanogens, and an elevated CH(inf4) emission were observed at the same time. (iii) The period of enhanced N(inf2)O emission corresponded to the increase in beta and omega hydroxy fatty acids derived from the outer membrane. Additionally, the continuous increase in branched-chain fatty acids suggested an increase in gram-positive bacteria and actinomycetes, and the decrease in polyunsaturated fatty acids indicated a decrease in eukaryotic cells during the composting.
TL;DR: In this article, a simple respirometric technique for the assessment of compost stability (SOUR test) was developed, that utilises a dissolved oxygen probe to measure changes in the oxygen concentration in an aqueous compost extract, under conditions ensuring optimum microbial activity and maximum reaction rates.
TL;DR: Some important methodological prerequisites for proper measurement and data interpretation are described, and a common scale and dimension of emission data are proposed so that data from different studies can be compared.
TL;DR: In this paper, a windrow composting pile, having the dimensions 2.5 m (height) x 30 m (length) was established, and the maturation of waste compost was accompanied by a decline in NH4+-N concentration, water soluble C (WSC) and an increase in NO3-N content.
Abstract: Composting of pruning waste, leaves and grass clippings was monitored by different parameters. A windrow composting pile, having the dimensions 2.5 m (height) x 30 m (length) was establish. The maturation of pruning waste compost was accompanied by a decline in NH4+-N concentration, water soluble C (WSC) and an increase in NO3–-N content. Both organic matter (OM) content and total N (TN) losses during composting followed a first-order kinetic equation. These results were in agreement with the microbiological activity measured either by the CO2 respiration or dehydrogenase (DH-ase) activity during the process. Statistically significant correlations were found between DH-ase activity, easily biodegradable organic C forms, NH4+-N and NO3–-N concentrations and organic matter content and N losses. For this reason, DH-ase activity and the CO2 evolution could be used as good indicators of pruning waste compost maturity. In contrast, humification parameters data from the organic matter fractionation did not agree with the initially expected values and did not contribute to the assessment of compost maturity. Neither the cation exchange capacity nor the germination index showed a clear tendency during the composting time, suggesting that these parameters are not suitable for evaluating the dynamics of the process.
TL;DR: It is concluded that BSF biowaste treatment offers an environmentally relevant alternative with very low direct GHG emissions and potentially high GWP reduction and further research should improve residue post-treatment.