TL;DR: To quantify the occurrence of ingested plastic in fish species caught at different geographical positions in the North Sea, and to test whether the fish condition is affected by ingestion of plastics, 1203 individual fish of seven common North Sea species were investigated.
Abstract: To quantify the occurrence of ingested plastic in fish species caught at different geographical positions in the North Sea, and to test whether the fish condition is affected by ingestion of plastics, 1203 individual fish of seven common North Sea species were investigated: herring, gray gurnard, whiting, horse mackerel, haddock, atlantic mackerel, and cod. Plastic particles were found in 2.6% of the examined fish and in five of the seven species. No plastics were found in gray gurnard and mackerel. In most cases, only one particle was found per fish, ranging in size from 0.04 to 4.8 mm. Only particles larger than 0.2 mm, being the diameter of the sieve used, were considered for the data analyses, resulting in a median particle size of 0.8 mm. The frequency of fish with plastic was significantly higher (5.4%) in the southern North Sea, than in the northern North Sea above 55°N (1.2%). The highest frequency (>33%) was found in cod from the English Channel. In addition, small fibers were initially detected in most of the samples, but their abundance sharply decreased when working under special clean air conditions. Therefore, these fibers were considered to be artifacts related to air born contamination and were excluded from the analyses. No relationship was found between the condition factor (size–weight relationship) of the fish and the presence of ingested plastic particles.
TL;DR: Alcalase and Neutrase showed optimum activity against Pacific whiting solid wastes (PWSW) at pH 95, 60 °C and pH 70, 55 °C, respectively Alcalase had a higher proteolytic activity than NEutrase Enzyme concentration, reaction time, and waste/buffer ratio significantly affected the hydrolysis and nitrogen recovery as mentioned in this paper.
Abstract: Alcalase and Neutrase showed optimum activity against Pacific whiting solid wastes (PWSW) at pH 95, 60 °C and pH 70, 55 °C, respectively Alcalase had a higher proteolytic activity than Neutrase Enzyme concentration, reaction time, and waste/buffer ratio significantly affected the hydrolysis and nitrogen recovery (NR) (p < 005) Optimum conditions for PWSW hydrolysis were 20 AU Alcalase/kg, 1 h reaction time, waste/buffer ratio of 1:1 (w/v) Correlation between the degree of hydrolysis (DH) and NR (R2 = 0970−0978) was high Freeze-dried hydrolysate was brownish yellow in color (L* = 5459, a* = 670, b* = 2789) and contained 277% moisture, 7997% protein, 1344% ash, and 383% lipid Amino acid composition of freeze-dried hydrolysate was similar to that of PWSW and Pacific whiting muscle but tryptophan was reduced to 2150% and 1474%, respectively Keywords: Waste; Pacific whiting; hydrolysate; Alcalase; Neutrase
TL;DR: In this paper, the authors showed that autolysis may be the main cause of physicochemical changes in Pacific whiting muscle proteins during iced storage, but no noticeable difference was observed in actin.
Abstract: No changes in actomyosin Ca 2+ -, Mg 2+ -, or Mg 2+ -Ca 2+ -ATPase activities were observed during iced storage of Pacific whiting fillets, but Mg 2+ -EGTA-ATPase increased with a loss of Ca 2+ -sensitivity. Surface hydrophobicity of actomyosin increased substantially within 2 days, but not total sulfhydryl (SH) content. During longer storage, the SH content decreased gradually, but surface hydrophobicity remained constant. Autolytic degradation products increased in fish muscle with storage time. Myosin heavy chain (MHC) was degraded by 45% within 8 days, but no noticeable difference was observed in actin. Results indicated that autolysis may be the main cause of physicochemical changes in Pacific whiting muscle proteins during iced storage.
TL;DR: A conceptual model suggests that photosynthetic activity and cell surface chemistry, together with the substantial surface area that arises from the great abundance of micron-sized cells, allow Synechococcus to dominate the annual whiting events in Fayetteville Green Lake.
Abstract: An annual whiting event occurs each year in late May to early June in Fayetteville Green Lake, New York. The initiation of this event correlates with exponential growth of the Synechococcus population within the lake. Synechococcus is the dominant (by approximately 4 orders of magnitude) autotrophic organism owing to the oligotrophic condition of the lake. The delta 13C values of the dissolved inorganic C range seasonally from -9.5% in winter to -6.2% in summer due to photosynthetic activity. Calcite precipitates principally in the microenvironment surrounding Synechococcus because of a photosynthetically driven alkalization process and the availability of the cells as nucleation sites. This calcite has a heavier delta 13C value (>4%) than does the dissolved inorganic C of the lake water owing to the cells' preferential uptake of 12C. A conceptual model suggests that photosynthetic activity and cell surface chemistry, together with the substantial surface area that arises from the great abundance of micron-sized cells, allow Synechococcus to dominate the annual whiting events in Fayetteville Green Lake.
TL;DR: Results indicate that fish rapidly migrate into beam trawled areas to feed on benthic animals that have been either damaged or disturbed by fishing or on scavenging invertebrates.
Abstract: The diets of gurnards Aspitrigla cuculus and Eutrigla gurnardus, lesser-spotted dogfish Scyliorhinus canicula and whiting Merlangius merlangus were examined to determine whether they migrated into recently trawled areas to feed on animals that may be damaged or dislodged by the action of a 4 m beam trawl. Gurnards and whiting increased their intake of prey after an area had been fished. In particular, they increased the proportion of the amphipod Ampelisca spinipes in their diets. Beam trawling damaged the purple burrowing heart urchin Spatanguspurpureus, scallop Aequipecten operculans, Ensisspp. and Laevocardium sp., exposing internal tissues which were then eaten by whit- ing. Some mobile invertebrate scavengers, such as Pandalusspp., only occurred in diets after the area had been fished, suggesting that these animals were also scavenging over the trawl tracks. Observa- tions of the seabed using a side-scan sonar revealed a greater concentration of fish marks around the trawl tracks than in adjacent unfished areas. Our results indicate that fish rapidly migrate into beam trawled areas to feed on benthic animals whlch have been either damaged or disturbed by fishing or on scavenging invertebrates. In areas where certain benthic communities occur, beam trawling inten- sity may be such that it creates a significant food resource for opportunistic fish species. This is a possi- ble mechanism whereby long-term community structure could be altered by fishing activity.