TL;DR: In this paper, the methanolic extract of V. pauciflora was semi-purified by using vacuum liquid chromatography (VLC) and further purified by HPLC to obtain a pure compound.
Abstract: Phytochemical studies on the woods of Vatica pauciflora and Vatica lowii have been conducted. The woods of V. pauciflora were extracted with methanol by using Soxhlet apparatus while the woods of V. lowii were extracted with acetone at room temperature. The methanolic extract of V. pauciflora was semi-purified by using vacuum liquid chromatography (VLC) and further purified by high performance liquid chromatography (HPLC) to obtain a pure compound (1) . The same technique of semi-purification has been employed on V. lowii acetone extract which was further purified by radial chromatography to give a pure compound (2) . Based on the UV, IR, NMR and mass spectral data, the isolated compounds were characterized as (-)- cis -resveratrol-10- C -b-D-glucopyranoside and (-)-piceid respectively.
TL;DR: In this article, Diphenylpicryl-hydrazyl (DPPH), Total Phenolic Content (TPC), Ferric Thiocyanate (FTC), and Thiobarbituric acid (TBA) methods were used to determine the antioxidant properties of the crude extracts.
Abstract: Ethyl acetate, methanol and ethanol crude extracts of the woods and twigs of Vatica pauciflora as well as the woods and the stem barks of Vatica lowii have been examined for their antioxidative properties. In this study, Diphenylpicryl-hydrazyl (DPPH), Total Phenolic Content (TPC), Ferric Thiocyanate (FTC) and Thiobarbituric acid (TBA) methods were used to determine the antioxidant properties of the crude extracts. A good correlation was observed among the DPPH, FTC and TBA results of the ethanol crude extract from the twigs of V. pauciflora (VTR) and acetone crude extract from the woods of V. lowii (VLBA). In conclusion, VTR and VLBA crude extracts had demonstrated the strongest antioxidant activity compared to other crude extracts in their species respectively.