Topic
Vanda testacea
About: Vanda testacea is a research topic. Over the lifetime, 14 publications have been published within this topic receiving 88 citations.
Papers
TL;DR: The study revealed that the endophytic fungal assemblage and diversity of B. neilgherrense and V. testacea of Kaiga forest of the Western Ghats were relatively similar between orchids and their tissues.
Abstract: We used standard isolation protocols to explore the endophytic fungal communities in three tissue types of two dominant orchids (Bulbophyllum neilgherrense and Vanda testacea) of the Kaiga forest of the Western Ghats. We surface sterilized and assessed 90 segments of each orchid for the occurrence and diversity of endophytic fungal taxa. The 118 fungal isolates were obtained from root, bulb and leaves of B. neilgherrense, consisting of 17 anamorphic taxa (range, 10–15 taxa) with 1.3 fungal taxa per segment (range, 1.2–1.4 taxa). Four taxa (Aspergillus flavus, A. niger, Penicillium sp. and morpho sp. 1) belonged to the core group (11.1%–32.2%). The relative abundance of A. flavus and morpho sp. 1 was more than 10%. A total of 130 fungal isolates from roots, stems and leaves of V. testacea yielded 20 anamorphic taxa (range, 11–15 taxa) with 1.4 fungal taxa per segment (range, 1.4–1.5 taxa). Aspergillus flavus, A. niger, A. ochraceus, Gliocladium viride, Penicillium sp. and morpho sp. 1 belonged to the core group. Relative abundance exceeded 10% for A. flavus, A. niger, and morpho sp. 1. The Simpson and Shannon diversity indices were higher in leaf than root or bulb/stem of both orchids. Jaccard’s similarity coefficient was higher between root and leaf in both orchids (56.3%-60%) than between other pairs. Our study revealed that the endophytic fungal assemblage and diversity of B. neilgherrense and V. testacea of Kaiga forest of the Western Ghats were relatively similar between orchids and their tissues.
44 citations
TL;DR: The best response in the explants (in terms of high regeneration frequency, early initiation, PLB proliferations, and plantlet development) was observed in 1.0 mg/l BAP alone/with 2.0mg/l NAA + activated charcoal.
Abstract: Foliar explants of Vanda testacea (Lindl.) Reichb. f. were cultured on Mitra (M) medium with 1.0 mg/l BAP, Kn each and 1.0 mg/l NAA individually and in combination for initiation of regeneration response, proliferation of regenerants and subsequent development of plantlets. Juvenility of the tissues and chemical stimulus were important factors in initiating the regeneration response in the explants. The relatively older leaf explants (>1cm in length) remained recalcitrant to regeneration the representing younger ones (<1cm in length) responded to certain chemical regimes. BAP, Kn individually in the medium should direct PLB regeneration whereas when used with NAA, the explants showed callus proliferation and further differentiated into PLBs. An individual treatment with NAA ( 1.0 mg/l ) impaired the response frequency and delayed further morphogenetic processes leading to plantlet development. The best response in the explants (in terms of high regeneration frequency, early initiation, PLB proliferations, and plantlet development) was observed in 1.0 mg/l BAP alone/with 1.0 mg/l NAA + activated charcoal. Plantlets were transferred to pots containing epiphytic compost (1 charcoal : 1 brick pices : 1 bats). Nearly 75% of plantlets survival was recorded. Key words : In vitro , Orchid, Vanda testacea , Micropropagation, Protocorm-like bodies, c allus D.O.I. 10.3329/ptcb.v19i1.4077 Plant Tissue Cult. & Biotech. 19 (1): 1-7, 2009 (June)
23 citations
TL;DR: Genomic template stability (GTS) test of hybrids were calculated indicating the percentage of descendant characteristics from parents and results indicate that identical species showed monophyletic group and genetic distances (D) that lead to the identification of Vanda, sp.
Abstract: Molecular data supporting morphological characters for identification and specific markers for ornamental Vanda species in Thailand can be achieved for economic means. The ten native Thai species that have been explored and identified are Vanda bensonii, Vanda brunnea, Vanda coerulea, Vanda coerulescens, Vanda denisoniana, Vanda pumila, Vanda lilacina, Vanda liouvillei, Vanda testacea and Vanda tessellata . Three unidentified species ( Vanda sp. 1, Vanda sp. 2 and Vanda sp. 3) have been discovered. In addition, three hybrids, hybrid 1 (maternal A × Vanda tessellata ), hybrid 2 ( Vanda denisoniana × Vanda bensonii ), and hybrid 3 (maternal B × paternal C), and two transferred species, Holcoglossum kimballianum (previously Vanda kimballiana ) and Papilionanthe teres (previously Vanda teres ) were included in genetic analysis by dendrogram constructed from random amplified polymorphic deoxyribonucleic acid (DNA) (RAPD) markers. The results indicate that identical species showed monophyletic group and genetic distances (D) that were between 0.15 to 0.17 which lead to the identification of Vanda, sp. 1 as Vanda bensonii and Vanda sp. 2 as Vanda brunnea because different species give D as higher as 0.20 to 0.40 with divided ancestors. Genomic template stability (GTS) test of hybrids were calculated indicating the percentage of descendant characteristics from parents. The GTS values of hybrid 2 compared with maternal and paternal were 32.88 and 36.62, respectively. Regarding hybrid 1 and 3 for which maternal and / or paternal are unclear, the GTS values when compared to other identified species ranged from 20.34 to 36.84 and 23.19 to 45.98, respectively. Finally, the barcodes of all wild studied species were done by two core barcodes and the tag sequences were tested for nucleotide variations of 0.005 to 0.076 in mat K and 0.007 to 0.040 in rbc L regions . The sequences were deposited in GenBank database with accession numbers. Key words : Genetic analysis, genomic template stability, mat K, rbc L, Orchidaceae, Vand ,.
18 citations
TL;DR: A simple dichotomous key based on the leaf and root anatomical characters for the identification of the south Indian Vanda species is provided.
16 citations
Journal Article•
TL;DR: If a well-established method is developed for their survival in the garden, it can be said that such orchid species can well be popularized and can be conserved forever.
Abstract: Though small in area, Nepal is considered to be rich in orchid flora. It has been realized that archid flora in Nepal has not been fully explored. Orchid in gardens, green houses and even in home decoration is not fully well appreciated due to difficulty in its assemblage, lack of knowledge in culture and garden varieties. Natural History Museum, Kathmandu has tried to accumulate some orchid species in its garden and in herbarium, attempting to create awareness for its conservation. In the present paper orchid of less attraction for garden development has been described from its collection. Some of such species are Eulophia dabia , Gastrochilus acutifolius , Coelogyne flavida , Cleisostoma filiforme , Vanda testacea , Dendrobium bicameratum and so on. If a well-established method is developed for their survival in the garden, it can be said that such orchid species can well be popularized and can be conserved forever.
Key words: Orchids, Natural History Museum; conservation
J. Nat. Hist. Mus. Vol.22, 2005 p.57-63
13 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2020 | 1 |
| 2018 | 2 |
| 2017 | 1 |
| 2014 | 2 |
| 2012 | 4 |
| 2010 | 2 |