TL;DR: PRF, which belongs to a new second generation of platelet concentrates, with simplified processing, and not requiring biochemical blood handling, has several advantages over traditionally prepared PRP, which has been widely used for accelerating soft tissue and hard tissue healing.
Abstract: To assess the potential use and benefits of Platelet-Rich Fibrin (PRF) over Platelet-Rich Plasma (PRP), for wound healing post oral and maxillofacial surgeries. This article describes the evolution of this second generation platelet concentrate and its multiple uses in various surgical procedures. Around 5 ml of whole venous blood is collected from the patients in each of the two sterile vacutainer tubes of 6 ml capacity without anticoagulant. The vacutainer tubes are then placed in a centrifugal machine at 3000 revolutions per minute (rpm) for 10 minutes, and the middle fraction containing the fibrin clot is then collected 2 mm below lower dividing line, to obtain the PRF. Cavities filled with PRF post oral and maxillofacial surgical procedures, at the institute, showed faster healing in half the time as compared to physiologic healing. PRF, which belongs to a new second generation of platelet concentrates, with simplified processing, and not requiring biochemical blood handling, has several advantages over traditionally prepared PRP, which has been widely used for accelerating soft tissue and hard tissue healing. However, the preparation being strictly autologous, the amount of PRF obtained is limited.
TL;DR: A new protocol is tested for bacterial identification from blood culture broths in hospital routine that correlates with conventional microbiology procedures by up to 90%, and by >95% for only monomicrobial samples, and provides a decreased turn-around time for identification of bacteria isolated from blood cultures.
TL;DR: Experimental evidence suggests that the poor yields were due to carrier 99Tc, and this effect may explain low labeling yields obtained with some other radiopharmaceuticals.
Abstract: A simple kit for the preparation of 99mTc-labeled red blood cells in a closed sterile system is described. Whole blood (3–6 ml) is automatically drawn from the patient into a 10-ml Vacutainer tube, containing a freeze-dried stannous citrate formulation, with heparin as an anticoagulant. The kit provides consistent 97% yields in 20 min with small blood samples. The radionuclide is added at the end of the labeling sequence to minimize operator exposure. Freeze dried kits have performed well after more than 12 months. Some technetium solutions produced low labeling yields. Experimental evidence suggests that the poor yields were due to carrier 99Tc. This effect may explain low labeling yields obtained with some other radiopharmaceuticals.
TL;DR: Improved procedures included increased baseline stability, shorter analysis time, and simpler standardization, which improved the sensitivity of the sensitive assay for carboxyhemoglobin based on reaction with K3Fe(CN)6 and gas chromatography of the liberated CO.
Abstract: We examined the sensitive assay for carboxyhemoglobin based on reaction with K3Fe(CN)6 and gas chromatography of the liberated CO. Our improvements included increased baseline stability, shorter analysis time, and simpler standardization. EDTA-containing Vacutainer Tubes (lavender-stoppered) increase the carboxyhemoglobin content of blood stored in them. The carboxyhemoglobin content of blood stored in capillary tubes containing solid heparin and saponin remained stable for two weeks. Using our improved procedures, we measured the carboxyhemoglobin content of blood from adults and neonates collected via venipuncture or heel or fingersticks. We observed no significant difference in carboxyhemoglobin content of blood obtained by venipuncture or heel stick for premature infants, 0.19 +/- 0.04 vs 0.18 +/- 0.03 mL of CO per 100 mL of blood, respectively (mean +/- SD). Nonsmoking adults (n = 19) had CO values (mean +/- SD) of 0.19 +/- 0.03 and 0.17 +/- 0.04 mL per 100 mL of blood, and smoking adults (n = 7) gave CO values of 0.96 +/- 0.49 and 0.91 +/- 0.49 mL/dL, for venipuncture and fingerstick, respectively.
TL;DR: Drawing blood through intravenous catheters was associated with significantly more hemolysis than drawing blood with straight needles, and using a combination of intravenousCatheter and vacutainer caused more hemorysis than using an intravenous Catheter with a syringe.