Abstract: The polymerase chain reaction (PCR) was used to develop a simple technique for detecting monoclonality at the DNA level in B lymphocyte populations in formalin fixed, paraffin wax embedded material. Sections were dewaxed and dehydrated, and the DNA was extracted by boiling in water for 45 minutes. A semi-nested PCR was performed to amplify the V-D-J region of the immunoglobulin heavy chain gene. The product was electrophoresed and viewed under ultraviolet light after ethidium bromide staining. Specimens from 26 B cell lymphomas produced a monoclonal band in 24 cases and no amplification in two cases; monoclonality was specific for this disorder. Specimens from seven T cell lymphomas produced no amplification; specimens from nine reactive nodes produced a broad smear of polyclonal material; and specimens from 12 cases of carcinoma produced either no amplification or polyclonal material. As detection of monoclonality is strongly suggestive of neoplastic disease, this technique is likely to be of value in routine diagnosis, because of its speed, simplicity, and applicability to fixed, embedded material.

Abstract: A flexible and relatively inexpensive method and apparatus are described for continuous pressure mapping of aerodynamic surfaces using photoluminescence and imaging techniques. Platinum octaethylporphyrin (PtOEP) has a phosphorescence known to be quenched by oxygen. When dissolved in a silicone matrix, PtOEP may be distributed over a surface as a thin, uniform film. When the film is irradiated with ultraviolet light, the luminescence intensity provides a readily detectable, qualitative surface flow visualization. Moreover, since the luminescence intensity is found to be inversely proportional to the partial pressure of oxygen, a quantitative measure of pressure change may be obtained using a silicon target vidicon or a charge-coupled device video sensor to measure intensity. Luminescent images are captured by a commercial frame buffer board. Images taken in wind tunnels during airflow are ratioed to images taken under ambient 'wind-off' conditions. The resulting intensity ratio information is converted to pressure using calibration curves of I0/I vs p/p0, where I0 is the intensity at ambient pressure p0 and I is the intensity at any other pressure p.

Abstract: Southern blot hybridization analysis of clonal antigen receptor gene rearrangements has proved to be a valuable adjunct to conventional methods for diagnosing lymphoid neoplasia. However, Southern blot analysis suffers from a number of technical disadvantages, including the time necessary to obtain results, the use of radioactivity, and the susceptibility of the method to various artifacts. We have investigated an alternative approach for assessing the clonality of antigen receptor gene rearrangements in lymphoid tissue biopsy specimens. This approach involves the amplification of rearranged gamma T-cell receptor genes by the polymerase chain reaction and analysis of the polymerase chain reaction products by denaturing gradient gel electrophoresis. By use of this approach, clonal rearrangements from neoplastic lymphocytes constituting as little as 0.1-1% of the total cells in the tissue are detected as discrete bands in the denaturing gel after the gel is stained with ethidium bromide and viewed under ultraviolet light. In contrast, polyclonal rearrangements from reactive lymphocytes appear as a diffuse smear along the length of the gel. Our findings suggest that polymerase chain reaction combined with denaturing gradient gel electrophoresis may offer a rapid, nonradioactive, and sensitive alternative to Southern blot analysis for the diagnostic evaluation of lymphoid tissue biopsy specimens.

Abstract: Ro/SS-A antibodies are found in a number of human autoimmune disorders including Sjogren's syndrome and several systemic lupus erythematosus-related disorders. These heterogeneous autoantibodies are known to recognize several distinct cellular antigens. With synthetic oligonucleotides corresponding to amino acid sequence information we have isolated a full-length cDNA clone which encodes a human Ro ribonucleoprotein autoantigen. The 1,890-base pair clone contains an open reading frame that encodes a 417-amino acid, 48-kD polypeptide that migrates aberrantly at 60 kD by SDS-PAGE. Rabbit antibodies raised against this protein's recently described amino-terminal epitope react with a previously identified 52-kD human Ro protein and immunoprecipitate the human cytoplasmic RNAs. Ultraviolet light cross-linking studies suggest that this Ro protein binds each of the four major human cytoplasmic RNAs. The deduced amino acid sequence is 63% homologous to an Onchocerca volvulus antigen. Southern filter hybridization analysis indicates that this gene is not highly polymorphic and exists as a single copy in the human genome. Chromosomal localization studies place this gene on the short arm of chromosome 19 near the gene encoding the low density lipoprotein receptor.

Abstract: The ability of local ultraviolet B (UVB) irradiation to impair the induction of dinitrofluorobenzene (DNFB)-specific contact hypersensitivity (CH) in mice has been shown to be genetically determined. We have explored the possibility that the mouse Tnfa and Lps loci are involved. We demonstrate that C3H/HeN (Lps n strains are UVB-susceptible, whereas C3H/HeJ (Lps d ) strains are UVB-resistant. Our results indicate that local intradermal (ID) injection of mouse recombinant tumor necrosis factor-alpha (TNFa) into sites painted with DNFB impaired the induction of CH, and in a dose response experiment the effect was found to be more marked in C3H/HeN than in C3H/HeJ. Systemic administration of neutralizing TNFa-specific antibody reconstituted the UVB-induced defect in induction of CH in UVB-susceptible mice, confirming that TNFa is a major mediator of the deleterious effects of UVB on induction of cutaneous immunity. The UVB-susceptibility trait (revealed by effects on CH) correlates positively with a recently described restriction fragment length polymorphism (RFLP) at the Tnfa locus (allele b) and with the wild-type Lps n allele. These results suggest that appropriate alleles at the Tnfa and Lps loci conspire to render mice susceptible to the impairment of CH induction by UVB. We propose that the mechanism may function through the capacity of UVB to elicit excessive local (cutaneous) production of TNFa, which mediates the immune defect.

Abstract: The invention relates to lenses, prisms or other optical members which are subjected to high-power ultraviolet light having a wavelength of about 360 nm or less, or ionizing radiation, particularly optical members for use in laser exposure apparatus for lithography, and to blanks for such optical members. The homogeneity of the refractive index distribution and the resistance to optical deterioration when the optical members are exposed for a long period of time to short wavelength ultraviolet light from a laser beam are improved. The optical members are made of high-purity synthetic silica glass material containing at least about 50 wt. ppm of OH groups, and are doped with hydrogen. The refractive index distribution caused by the fictive temperature distribution during heat treatment in the process of producing high-purity silica glass blanks for optical members in accordance with the present invention is offset by the combined refractive index distribution determined by the OH group concentration distribution or by the OH group concentration distribution and the Cl concentration distribution in the glass.

Abstract: An integrated circuit amplifier having a random input offset voltage is adaptable such that the input offset voltage may be cancelled out. An inverting input node is a floating input node and is coupled to a source of input signal by a first capacitor. A second capacitor is connected between the output of the amplifier and the floating node. An ultraviolet window above the second capacitor allows the floating node to be charged, by the application of ultraviolet light, to a voltage which effectively cancels the input offset voltage. The ultraviolet window and capacitor electrodes are arranged such that the ultraviolet light may strike only the desired areas of the structure.

Abstract: Acute ultraviolet light B (UVB) injury is associated with dermal mast cell histamine release. The possibility that histamine-stimulated prostaglandin (PG) synthesis could be a mechanism for irradiation erythema was therefore examined using human skin explants. Explants responded to UV irradiation (120 mJ/cm2) with a fivefold increase in synthesis of prostaglandins E2, F2 alpha and 6-keto PGF1 alpha. Incubating explants with the H1 antihistamines brompheniramine (50 microM) or pyrilamine (30 microM) inhibited PG release from irradiated explants 63 +/- 4.9% (mean +/- SEM) 6 h after UV exposure. Antihistamines did not affect PG synthesis in control explants. Irradiation increased the histamine concentration in explant conditioned medium only 50% over basal values, suggesting that irradiation enhanced histamine responsiveness. Explants were therefore incubated with exogenous histamine. In irradiated explants, PG synthesis was stimulated threefold by 3 microM histamine. Unirradiated explants' PG synthesis was unaffected by histamine. Enhanced histamine sensitivity was also examined in epidermal cell cultures. In irradiated cultures, histamine sensitivity was again markedly potentiated: as little as 1 microM histamine stimulated significant PGE2 release and the response to 10-30 microM histamine was increased six to eight times compared with that of unirradiated cultures. These studies demonstrate that endogenous histamine stimulates PG synthesis in human skin after UV injury by potentiation of histamine-induced prostaglandin release. Potentiated agonist responses induced by UV exposure may contribute to the effects of UVB irradiation injury and in particular to irradiation erythema.

Abstract: Highly weather and corrosion resistant flexible reflective films for solar energy applications and lighting reflectors are formed by vapor-depositing silver onto a flexible support sheet, e.g., biaxially oriented polyester film and adherently bonding a protective fluorocarbon film, e.g., poly(vinyl fluoride), poly(vinylidine fluoride), or poly(tetrafluoroethylene), to the surface of the silver distal to the flexible support sheet with a layer of adhesive. Fluorocarbon film is highly optically transmissive to visible, ultraviolet, and/or near infra-red light between about 300-2,500 nanometers, stable to ultraviolet light, impervious to moisture, non-hygroscopic, scratch resistant, and easy to keep clean. The adhesive is highly optically transmissive to light between about 300-2,500 nanometers and stable to ultraviolet light. Chemical corrosion inhibitors and UV absorbers incorporated into the flexible reflective film can increase its resistance to corrosion and degradation by ultraviolet light.

Abstract: Escalating doses of recombinant interferon alfa-2a (Roferon-A), administered intramuscularly three times weekly, combined with psoralen plus ultraviolet light irradiation (PUVA), were tested in a phase I trial for the therapy of patients with cutaneous T-cell lymphomas (CTCL). Interferon doses were escalated in groups of three patients from 6 million to 30 million IUs three times weekly. Disease stages ranged from IB to IVB. Eighty percent of the patients entered in this trial had failed at least one prior therapy. Complete remissions were obtained in 12 of 15 patients, and partial responses were seen in two of 15 patients, for an overall response rate of 93%. The median duration of response exceeded 13 months (range, 3-15+). All patients who responded have been maintained on therapy. The dose-limiting toxic effects were constitutional symptoms such as fevers and malaise (93.3%), leukopenias (40.0%), mental status changes consisting of depression and confusion (33.3%), and photosensitivity (26.6%). These side effects were reversible with a decrement in dose or discontinuation of the interferon. No patient tolerated 30 million IU of the interferon for extended periods; the maximally tolerated dose was 18 million IU. Interferon plus PUVA appears to be a highly effective regimen for the treatment of patients with CTCL. Phase II studies investigating this combination, using 18 million IU of interferon alfa-2a three times weekly, should be undertaken to expand these findings, and to attempt to reduce the toxic effects associated with this therapy.

Abstract: This study investigates the chemistry of femoral gland secretions in the desert iguana,Dipsosaurus dorsalis (Lacertilia: Iguanidae), and discusses their possible functional significance. Electrophoretic and proton NMR studies indicated that the secretions are composed of approximately 80 % protein and 20% lipid material. Individual differences in polyacrylamide gel banding patterns of femoral gland proteins were found. Reflectance spectroscopy revealed that the secretions strongly absorb longwave ultraviolet light, a feature that may be important in the localization of secretion deposits in the environment.

Abstract: A stable topical composition consists essentially of at least about 1 wt.% L-ascorbic acid, preferably from about 3 to 20 wt. % L-ascorbic acid, and more preferably about 5 to 10 wt. % L-ascorbic acid in water and a carrier for topical application is disclosed. Such a composition appears to allow ready penetration into the skin and retards the damage caused by ultraviolet light. Any composition containing at least about 1 wt. % L-ascorbic acid is expected to have beneficial effects, with the concentration of L-ascorbic acid being primarily limited by its solubility in the water/carrier system. The ratio of water to carrier is at least 1:1, and more preferably from 2:1 to 10:1, and the pH of the composition is no more than 3.0 to 3.5, preferably no more than about 2.5.

Abstract: Complex organic molecules, such as polychlorinated biphenyls can be degraded on porous titanium ceramic membranes by photocatalysis under ultraviolet light.

Abstract: DNA damage caused by UV-B and UV-A irradiation and the rate of repair of such damage was quantitated in bovine lens epithelial cell cultures using a modified alkaline elution methodology. Two enzymes, bacteriophage T4 endonuclease V, which cleaves at the site of pyrimidine dimers, and E. coli endonuclease III, which cleaves at the site of thymine glycols, were utilized. Pyrimidine dimers were not detected after UV-A irradiation of lens cultures with up to 400 J/m2. In contrast, after exposure to as little as 2 J/m2 of UV-B irradiation, large numbers of pyrimidine dimers were observed. At higher fluences, thymine glycols were also found. Significant levels of DNA-DNA crosslinking were suggested by reduced rates of elution of DNA from cells treated with both UV-B irradiation and H2O2 in comparison to treatment with H2O2 alone. Protein-DNA crosslinks, in contrast, were not observed. The rate of repair of UV-B induced DNA damage was quantitated by harvesting cells at various times after the UV-B exposure. Single-strand breaks were never observed immediately after UV-B exposure but appeared later during the repair phase. In contrast to the repair of H2O2 induced DNA damage, which is largely completed within 30 min of exposure, more than 50% of the UV-B light induced DNA damage remained unrepaired five hours after exposure. This difference between the rate of repair of H2O2 and UV-B induced DNA damage could provide valuable insights into the nature of DNA damaging agents in the lens environment and may reflect underlying differences in the potential for epithelial cell DNA mutation in response to various DNA damaging insults.

Abstract: An integrated circuit amplifier having a random input offset voltage is adaptable such that the input offset voltage may be cancelled out. An inverting input node is a floating input node and is coupled to a source of input signal by a first capacitor. A second capacitor is connected between the output of the amplifier and the floating node. An ultraviolet window above the second capacitor allows the floating node to be charged, by the application of ultraviolet light, to a voltage which effectively cancels the input offset voltage. The ultraviolet window and capacitor electrodes are arranged such that the ultraviolet light may strike only the desired areas of the structure.

Abstract: A point of use water purification system including a housing supporting an untreated water container, a removable and disposable filter means and a treated water receiving container all of which are self contained in relation to the housing to enable the housing to be supported from a countertop or other suitable surface at the point of use. The filter means includes multiple compartments with purification media therein with two of the compartments including plate means coated with a photoactivated catalyst, preferrably titanium oxide (TiO 2 ). An ultraviolet lamp and reflector directs ultraviolet light energy onto the catalyst. Flow control means is provided in the filter means to provide a slow rate of flow thereby providing long time contact between the water being treated and the purification media in the filter means to provide maximum purification efficiency. The water supply container is removable and refillable to provide a batch purification process well adapted for installation at a point of use in both domestic and industrial applications.

Abstract: A method for coating a plastic lens element including applying a coating composition consisting substantially of reactants having at least triacrylate functionality, a photo-initiator and a polymerization inhibitor reactive with oxygen to the face of a mold. The coating is subjected to ultraviolet light in an oxygen containing environment such that a hard abrasion-resistant coating is formed. The mold is then filled with a lens forming composition which is reactive with acrylate groups of the coating at the coating/lens interface. The lens forming composition is permitted to cure in the mold with the lens forming composition being bonded to the lens coating.

Abstract: Both naturally occuring and carcinogen-induced tumors display not only point mutations in cellular oncogenes but also more complex changes in cellular oncogenes and other cellular genes. For this and other reasons, it seems likely that DNA damage in mammalian cells can induce alterations in gene expression that may have both short and long term consequences in the target cell. The purpose of this review is to summarize current available information on inducible responses to UV-irradiation and other mediators of DNA damage in mammalian cells, and to provide some working hypotheses. We have divided these responses into three time frames, immediate (0–12 hours), early (12–48) and late (beyond 48 hours). Immediate responses include the action of DNA repair enzymes, some of which are induced as a consequence of DNA damage, and transient inhibition of DNA synthesis. Within the past few years considerable evidence has accumulated that during this immediate period there is increased expression of certain cellular oncogenes, proteases and proteins whose functions remain to be identified. It is of interest that the expression of some of these genes is also induced by certain growth factors, tumor promoters and heat shock. Alterations in gene expression during the subsequent “early” period (12–48 hrs.) have not been studied in detail, but it is during this period that one can detect increased replication of several types of viruses in cells that harbor these viruses. We have examined in detail the induction of asynchronous polyoma DNA replication (APR) in a rat fibroblast cell line carrying integrated copies of this DNA. We have obtained evidence that UV-irradiation of these cells leads to the synthesis of a 40 kd protein, within the first 1–24 hrs after irradiation, that binds to a specific sequence TGACAACA in the regulatory region of polyoma DNA. We suggest that this protein acts together with other proteins to induce APR and that this serves as a useful model for understanding the mechanisms responsible for amplification of cellular genes, a phenomenon often seen in malignant tumors. Finally, we discuss how the events occurring during the immediate and early periods following DNA damage might lead to late effects in the target cell that are stable and contribute to the genotype and phenotype of some of the progeny of these cells that are destined to become tumor cells.

Abstract: : The resistance of Coxiella burnetii to physical and chemical stress is well documented. Compared with vegetative bacteria and other rickettsiae, this hardy microorganism resists elevated temperatures, desiccation, osmotic shock, ultraviolet light, and chemical disinfectants. The environmental stability of C. burnetii is perhaps related to (i) the sporulation process, which produces endogenous spores and small dense cells, and (ii) the metabolic dormancy of C. burnetii at neutral pH C. burnetii is very stable and easily transmitted in aerosols. Estimates of infectivity for humans range from 1 to 10 microorganisms. Survival of the microorganisms for long periods in contaminated foods, buildings, and pastures has resulted in human and animal infections.

Abstract: A photoresist system that is easily structurable and, in particular, is suitable for the deep ultraviolet range is provided. An increased etching resistance to a halogen-containing plasma is produced in a lithographically generated photoresist structure by treatment with a reactant. The reactant comprises predominantly aromatic structures and includes reactive groups that are suitable for chemical reaction with further reactable groups of the photoresist. In an embodiment, the photoresist includes anhydride or epoxy groups that are suitable for structuring with deep ultraviolet light.

Abstract: An integrated circuit amplifier having a random input offset voltage is adaptable such that then input offset voltage may be cancelled out. An inverting input node is a floating input node and is coupled to a source of input signal by a first capacitor. A second capacitor is connected between the output of the amplifier and the floating node. An ultraviolet window above the second capacitor allows the floating node to be charged, by the application of ultraviolet light, to a voltage which effectively cancels the input offset voltage. The ultraviolet window and capacitor electrodes are arranged such that the ultraviolet light may strike only the desired areas of the structure.

Abstract: We have identified a strain of the yeast Pichia acaciae which produces a “killer” toxin active against the yeast Debaryomyces tamarii. The killer phenotype was associated with the presence of two DNA plasmids, pPacl-1 (13.6 kilobase pairs) and pPacl-2 (7.3 kilobase pairs). P. acaciae strains, cured of these plasmids by irradiation with ultraviolet light, lacked killer activity and were sensitive to toxin produced by the parental strain. A partially cured strain, GS-1215, missing only the smaller plasmid, pPacl-2, also exhibited loss of both toxin activity and immunity. Exonuclease studies revealed that both plasmids were linear double-stranded DNA molecules with 5′ protected ends. The P. acaciae system differs from that of the well-studied Kluyveromyces lactis “killer” system both in the range of susceptible strains and in the sizes of the plasmids involved. Our studies contradict previous reports that Pichia killer systems are invariably chromosomal.

Abstract: In 1989 more than 500,000 new cases of nonmelanomatous skin cancer were dialgnosed in the United States. The majority of these skin cancers are basal cell carcinomas and the majority of the remainder are squamous cell carcinomas. From an etiological point of view the most common cause is exposure to ultraviolet light and many treatment modalities are used in the management of these lesions. They are readily accessible to examination, thereby offering a great potential for early diagnosis and cure. Radiation therapy plays a significant role as the major treatment modality in lesions involving the eyelid, the nose, the lips, and the ea.rlobes. The major reason for the utilization of radiotherapy in these areas is the preservation of normal soft tissues, thereby giving rise to excellent cosmetic results and excellent control rates. Radiotherapy is equal to surgical management in other sites and is therefore an equally competing treatment regimen. The basic advantages for well-designed programs of radiation therapy are directed toward excellent cosmetic results and a major potential for cure. In this issue Lovett ef al. report their experience from the Radiation Oncology Center of the Mallinckrodt Institute of Radiology at the Washington University School of Medicine (1). This experience extended from January 1966 through December 1986 and comprised 339 consecutively treated biopsied proved squamous cell and basal cell carcinomas of the skin. There were 242 basal cell carcinomas, 92 squamous cell carcinomas, and 5 variances of squamous cell carcinomas in various locations. Radiotherapy was the initial treatment modality in 2 12 patients, and 127 were .treated after having failed initial surgical excision. Lymph node metastases in patients with basal cell carcinoma occurs in less than 0.5% of all patients whereas metastatic disease to lymph nodes from primary treated squamous cell carcinomas occurs in 15% and 39% of patients with recurrent sqeamous cell carcinoma of the skin. Distant metastatic disease was found in only one patient in the entire group. The data presented by Lovett et al. indicate local tumor control was achieved in 86% of the patients treated, 9 1% of those having basal cell carcinomas and 75% of those having squamous cell carcinoma. The data presented clearly stress the impact of tumor size on the potential for local control: 97% for basal cell carcinomas less than 1 cm in diameter and 9 1% for squamous cell carcinomas less than 1 cm in diameter; the local control rate diminished as the tumor size increased. For tumors greater than 5 cm, the tumor control for basal cell carcinomas was 87% and 56% for squamous cell carcinomas. The modality used in treating the patients was clearly a significant and important predictor in the expected outcome. When superficial X rays were used, the tumor control was excellent for all tumor sizes. The utilization of superficial X rays allows for a wide variety in choice of field size and half-value layers, and provides excellent dose distribution within the volume being treated. Superficial X ray techniques allow for sophisticated shielding procedures to be used thus shielding normal structures with a more precise fitting of the central portion of the beam to the area being treated and for choice of energy and filtration to allow for homogeneous adequate dose distribution within the volume of the tumor. In the utilization of electron beam, however, major factors emerge in the treatment planning that can have a significant and important impact upon the outcome. The data from Lovett et al. indicate that tumor control was 88% for tumors less than 1 cm, 72% for those that were l-5 cm in size, and 78% for those that were greater than 5 cm in size, results that were clearly less good than those achieved by superficial X rays. Treatment planning in the utilization of electron beam must consider the utilization of highly sophisticated techniques for shielding normal structures but also must take into consideration the specific characteristics of the electron beam and the need for a bolus in the field being treated to allow for homogeneous