TL;DR: This study reports a simple non-invasive method for establishing primary cell lines from Australian dragon lizards without sacrifice and finds no reduction in cell viability after short term (<6 months) storage at −80 °C.
Abstract: Primary cell lines were established from cultures of tail and toe clips of five species of Australian dragon lizards: Tympanocryptis pinguicolla, Tympanocryptis sp., Ctenophorus fordi, Amphibolurus norrisi and Pogona vitticeps. The start of exponential cell growth ranged from 1 to 5 weeks. Cultures from all specimens had fibroblastic morphology. Cell lines were propagated continuously up to ten passages, cryopreserved and recovered successfully. We found no reduction in cell viability after short term (<6 months) storage at −80 °C. Mitotic metaphase chromosomes were harvested from these cell lines and used in differential staining, banding and fluorescent in situ hybridisation. Cell lines maintained normal diploidy in all species. This study reports a simple non-invasive method for establishing primary cell lines from Australian dragon lizards without sacrifice. The method is likely to be applicable to a range of species. Such cell lines provide a virtually unlimited source of material for cytogenetic, evolutionary and genomic studies.
TL;DR: The systematic status of the dragon lizards Tympanocryptis lineata pinguicolla and T. tetraporophora (Agamidae) was examined by allozyme electrophoresis and multivariate morphological techniques, confirming their recognition at the specific level and raising their status to full specific status.
Abstract: The systematic status of the dragon lizards Tympanocryptis lineata pinguicolla and T. tetraporophora (Agamidae) was examined by allozyme electrophoresis and multivariate morphological techniques. Both taxa exhibited considerable genetic and morphological differentiation from each other and from most other members of the T. lineata species group examined. The recognition of T. tetraporophora at the specific level is confirmed and T. l. pinguicolla is raised to full specific status. The remaining subspecies of T. lineata are each genetically well differentiated from each other and may represent full species. Allozyme analysis of specimens from the T. cephalus/intima complex in eastern Australia was problematic because of small samples and heterogenous distributions of alleles, a more extensive sampling being required to resolve their status. A key is provided for identification of species of Tympanocryptis in south-eastern Australia.
TL;DR: In the small Australian dragon (agamid) lizard Tympanocryptis tetraporophora mean snout-vent length (SVL) does not differ significantly between the sexes within the species as a whole, however, in a large sample from Sturt National Park, in northwestern New South Wales, males are larger than females both in snouted length and in mass.
Abstract: In the small Australian dragon (agamid) lizard Tympanocryptis tetraporophora mean snout-vent length (SVL) does not differ significantly between the sexes within the species as a whole. However, in a large sample from Sturt National Park, in northwestern New South Wales, males are larger than females both in snout-vent length and in mass. Large specimens are notably lacking from collections made during the coolest time of year.The number of premaxillary teeth is not correlated with head size. However, the number of acrodont teeth on the maxilla and dentary increases with head size.The modal number of presacral vertebrae is 23. There is no sexual dimorphism in either the number of postsacral vertebrae or the number of postsacral vertebrae with transverse processes. Hyperphalangy occurs in 3.2% of specimens. Gross variation in the sacral vertebrae occurs in 5.3% of specimens.For allometric analysis, two samples were considered: specimens from Stud National Park and all other specimens. Head length and hind l...
TL;DR: Two new subspecies of Tympanocryptis lineata Peters are described: T. l.
Abstract: Two new subspecies of Tympanocryptis lineata Peters are described: T. l. houstoni from the Nullarbor Plain and T. l. macra from the Kimberley and neighbouring part of Northern Territory. Tympanocryptis tetraporophora Lucas and Frost and T. uniformis Mitchell are removed from the Western Australian list, and T. cephala gigas Mitchell is merged in T. cephala Giinther.