Topic
TRPM3
About: TRPM3 is a research topic. Over the lifetime, 213 publications have been published within this topic receiving 9871 citations. The topic is also known as: GON-2 & LTRPC3.
Papers published on a yearly basis
Papers
TL;DR: It is shown by single channel studies that polycystin-2 behaves as a calcium-activated, high conductance ER channel that is permeable to divalent cations and thatpolycystic kidney disease results from the loss of a regulated intracellular calcium release signalling mechanism.
Abstract: Polycystin-2, the product of the gene mutated in type 2 autosomal dominant polycystic kidney disease (ADPKD), is the prototypical member of a subfamily of the transient receptor potential (TRP) channel superfamily, which is expressed abundantly in the endoplasmic reticulum (ER) membrane. Here, we show by single channel studies that polycystin-2 behaves as a calcium-activated, high conductance ER channel that is permeable to divalent cations. Epithelial cells overexpressing polycystin-2 show markedly augmented intracellular calcium release signals that are lost after carboxy-terminal truncation or by the introduction of a disease-causing missense mutation. These data suggest that polycystin-2 functions as a calcium-activated intracellular calcium release channel in vivo and that polycystic kidney disease results from the loss of a regulated intracellular calcium release signalling mechanism.
720 citations
TL;DR: Evidence is provided that TRPM3 functions as a chemo- and thermosensor in the somatosensory system and an unanticipated role for TR PM3 as a thermosensitive nociceptor channel implicated in the detection of noxious heat is revealed.
567 citations
TL;DR: It is shown that TRPM3, a divalent-permeable cation channel, is rapidly and reversibly activated by extracellular pregnenolone sulphate, a neuroactive steroid, enabling unanticipated crosstalk between steroidal and insulin-signalling endocrine systems.
Abstract: Transient receptor potential (TRP) cation channels are renowned for their ability to sense diverse chemical stimuli. Still, for many members of this large and heterogeneous protein family it is unclear how their activity is regulated and whether they are influenced by endogenous substances. On the other hand, steroidal compounds are increasingly recognized to have rapid effects on membrane surface receptors that often have not been identified at the molecular level. We show here that TRPM3, a divalent-permeable cation channel, is rapidly and reversibly activated by extracellular pregnenolone sulphate, a neuroactive steroid. We show that pregnenolone sulphate activates endogenous TRPM3 channels in insulin-producing beta cells. Application of pregnenolone sulphate led to a rapid calcium influx and enhanced insulin secretion from pancreatic islets. Our results establish that TRPM3 is an essential component of an ionotropic steroid receptor enabling unanticipated crosstalk between steroidal and insulin-signalling endocrine systems.
365 citations
TL;DR: The pore region of TRPV4 is delineated and a first insight into the possible architecture of its permeation pathway is given.
322 citations
TL;DR: These results show that alternative splicing can be a mechanism to produce channels with very different selectivity profiles, and unambiguously show that TRPM3 proteins constitute a pore-forming channel subunit and localize the position of the ion-conducting pore within theTRPM3 protein.
228 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 8 |
| 2020 | 17 |
| 2019 | 13 |
| 2018 | 9 |
| 2017 | 15 |
| 2016 | 6 |