Topic
TRNA 5'-end processing
About: TRNA 5'-end processing is a research topic. Over the lifetime, 3 publications have been published within this topic receiving 54 citations.
Papers
TL;DR: Biochemical studies indicate that PRORP2 requires Mg(2+) for catalysis and catalyzes the maturation of nuclear encoded substrates up to 10-fold faster than mitochondrial encoded precursor nad6 t-element under single-turnover conditions and it is demonstrated that PR ORP2 preferentially binds precursor tRNAs containing short 5' leaders and 3' trailers.
35 citations
TL;DR: A microarray containing probes that tile all known yeast noncoding RNAs found a general loss of Box C/D snoRNAs in the TetO7-BCD1 mutant and identified a relationship between tRNA 5′ end processing and tRNA splicing, processes that were previously thought to be independent.
Abstract: We used a microarray containing probes that tile all known yeast noncoding RNAs (ncRNAs) to investigate RNA biogenesis on a global scale The microarray verified a general loss of Box C/D snoRNAs in the TetO7-BCD1 mutant, which had previously been shown for only a handful of snoRNAs We also monitored the accumulation of improperly processed flank sequences of pre-RNAs in strains depleted for known RNA nucleases, including RNase III, Dbr1p, Xrn1p, Rat1p and components of the exosome and RNase P complexes Among the hundreds of aberrant RNA processing events detected, two novel substrates of Rnt1p (the RUF1 and RUF3 snoRNAs) were identified We also identified a relationship between tRNA 5′ end processing and tRNA splicing, processes that were previously thought to be independent This analysis demonstrates the applicability of microarray technology to the study of global analysis of ncRNA synthesis and provides an extensive directory of processing events mediated by yeast ncRNA processing enzymes
30 citations
TL;DR: The precise functional equivalence between RNP RNase P and PRORP despite such obvious difference in structure and ancestry makes them an excellent context in which to consider fundamental principles of enzyme function and to gain a deeper understanding of the role ofRNase P in the RNA metabolism.
8 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2016 | 2 |
| 2005 | 1 |