Topic
Traumatic acid
About: Traumatic acid is a research topic. Over the lifetime, 34 publications have been published within this topic receiving 1543 citations. The topic is also known as: 2E-dodecenedioic acid & trans-2-dodecenedioic acid.
Papers
TL;DR: The results indicated that the compound identified as traumatic acid is formed by autooxidation of trans- 10-ODA and that trans-10- ODA is a natural compound with growth-regulating properties.
Abstract: 12-Oxo- trans -10-dodecenoic acid ( trans -10-ODA) is an oxidation product of polyunsaturated fatty acids in plant tissues. The structural similarity of trans -10-ODA and traumatic acid, a compound considered to be a wound hormone, suggested that trans -10-ODA might be a precursor of traumatic acid. Both trans -10-ODA and traumatic acid were active in the Wehnelt bean assay. The results were more consistent with trans -10-ODA than with traumatic acid. Cucumber ( Cucumis sativus L. var. National Pickling) hypocotyls also showed a growth increase following treatment with trans -10-ODA, which suggested that trans -10-ODA has a more general influence on plant development than previously ascribed to traumatic acid. Runner beans ( Phaseolus vulgaris L. var. Kentucky Wonder) were analyzed for the presence of endogenous trans -10-ODA and traumatic acid. These are the beans from which traumatic acid was originally isolated in 1939. They contained trans -10-ODA but no traumatic acid. Young beans were a better source of trans -10-ODA than older beans and an increase in the esterified form of trans -10-ODA with age may have been due to a conversion of the free acid to the esterified form. The amount of endogenous trans -10-ODA increased when bean pod tissue was sliced and wounded. Rapid stirring and the presence of oxygen increased autooxidation of trans -10-ODA to traumatic acid in runner beans, which indicated that the compound identified as traumatic acid is formed by autooxidation of trans -10-ODA and that trans -10-ODA is a natural compound with growth-regulating properties. Enzyme extracts of runner beans synthesized trans -10-ODA from linoleic acid. No enzymic synthesis of traumatic acid was observed even when cofactors were added to the reaction mixture. This confirmed the conclusion that traumatic acid is formed by autooxidation of trans -10-ODA.
196 citations
TL;DR: Data suggest that in response to auxin depletion, endogenous mJA could be produced and act by linking physiological events thus leading to alkaloid biosynthesis activation.
Abstract: The effect of methyl jasmonate (mJA), jasmonic acid and traumatic acid, derivatives of the octadecanoic pathway, on the production of alkaloids by cell suspension cultures of Catharanthus roseus L. (G) Don was investigated. Cells cultured in the presence of auxin (m-cells) did not accumulate alkaloids. The addition of exogenous mJA to mcells restored the ability to produce alkaloids. In cells cultured in a 2,4-D-starved medium (p-cells), exogenous mJA greatly increased alkaloid production. Similar data were obtained for jasmonic acid. In contrast, traumatic acid had no effect on alkaloid production. The sensitivity of cell suspension cultures to exogenous mJA was restricted to the first four days of subculture corresponding to the active growth phase, whereas the alkaloid accumulation occurred only during the stationary phase of the subculture (days 6 to 10). When p-cells were treated with octadecanoic pathway inhibitors, the ability to produce alkaloids was strongly reduced. The addition of exogenous mJA always restored the ability to produce alkaloids. These data suggest that in response to auxin depletion, endogenous mJA could be produced and act by linking physiological events thus leading to alkaloid biosynthesis activation.
103 citations
TL;DR: Among these compounds, compounds 1, 2, 3, 6, 7, 9 and 12 had strong growth inhibitory effects on cancer cell lines, indicating that jujube extracts exhibited cytotoxicity on these cancer cell Lines.
Abstract: The fruit of Ziziphus jujuba Mill., also called hongzao in Chinese, has a long history of cultivation in China. From the fruit of Z. jujuba, twenty-seven known compounds were isolated and identified as the main constituents of these fruits. They were 3-O-(trans-p-coumaroyl)-alphitolic acid (1), 3-O-(cis-p-coumaroyl)-alphitolic acid (2), 3β-O-(trans-p-coumaroyl)-maslinic acid (3), pomonic acid (4), 2-oxo-pomolic acid (5), benthamic acid (6), terminic acid (7), oleanic acid (8), betulinic acid (9), quercetin 3-O-rutinoside (10), quercetin 3-O-robinobioside (11), apigenin (12), traumatic acid (13), (Z)-4-oxotetradec-5-enoic acid (14), 7(E)-9-keto-hexadec-7-enoic acid (15), 9(E)-11-oxo-octadecenoic acid (9CI) (16), and magnoflorine (27), etc. The HPLC fingerprint of Z. jujuba fruits was established at the same time. Compounds 4, 5, 7, 11, 14, 15 and 16 were isolated from Z. jujuba for the first time. Compound 14 was isolated from the nature for the first time. Furthermore, cytotoxicity against four human tumor cell lines (MCF-7, A549, HepG2 and HT-29) of the isolated compounds (1–17 and 27) was evaluated. Among these compounds, compounds 1, 2, 3, 6, 7, 9 and 12 had strong growth inhibitory effects on cancer cell lines. These results indicated that jujube extracts exhibited cytotoxicity on these cancer cell lines.
74 citations
TL;DR: The mechanisms of resistance in barley to fusarium head blight (FHB), caused by Gibberella zeae are complex and metabolomics technology was explored to phenotype resistance.
Abstract: The mechanisms of resistance in barley to fusarium head blight (FHB), caused by Gibberella zeae are complex. Metabolomics technology was explored to phenotype resistance. Spikelets of barley genotypes with contrasting levels of resistance to FHB, mock inoculated or with the pathogen, were extracted with aqueous methanol and the metabolites were analyzed using liquid chromatography and hybrid mass spectrometry. Peaks were de-convoluted using XCMS and annotated using CAMERA and IntelliXtract bioinformatics tools. A t-test, of a total of 1608 purified peaks, selected 626 metabolites with significant treatment effects, of which 161 were identified as resistance related (RR) metabolites. A total of 53 metabolites, that are RR or pathogenicity related (PR), were assigned with putative compound names. These mainly belonged to three metabolic pathways: fatty acid (jasmonic acid, methyl jasmonate, 9,10- dihydro-isojasmonate, linolenic acid, linoleic acid, traumatic acid), phenylpropanoid (p-coumaric acid, caffeyl alcohol, dimethoxy-4-phenylcoumarin, rosmarinic acid, diphyllin, 5-methoxypodophyllotoxin) and flavonoid (naringenin, catechin, quercetin, and alpinumisoflavone). A few PR/RR metabolites significantly reduced mycelial growth of G. zeae in vitro.
73 citations
TL;DR: The present findings reveal that TA exhibits multiple and complex activity in fibroblast cells in vitro, with its activity similar to unsaturated fatty acids, shows antioxidant and stimulatory effects on collagen biosynthesis.
Abstract: Traumatic acid (TA) is a plant hormone (cytokinin) that in terms of chemical structure belongs to the group of fatty acids derivatives. It was isolated from Phaseolus vulgaris. TA activity and its influence on human cells and organism has not previously been the subject of research. The aim of this study was to examine the effects of TA on collagen content and basic oxidative stress parameters, such as antioxidative enzyme activity, reduced glutathione, thiol group content, and lipid peroxidation in physiological conditions. The results show a stimulatory effect of TA on tested parameters. TA caused a decrease in membrane phospholipid peroxidation and exhibited protective properties against ROS production. It also increases protein and collagen biosynthesis and its secretion into the culture medium. The present findings reveal that TA exhibits multiple and complex activity in fibroblast cells in vitro. TA, with its activity similar to unsaturated fatty acids, shows antioxidant and stimulatory effects on collagen biosynthesis. It is a potentially powerful agent with applications in the treatment of many skin diseases connected with oxidative stress and collagen biosynthesis disorders.
53 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 1 |
| 2016 | 2 |
| 2014 | 1 |
| 2012 | 1 |
| 2011 | 3 |
| 2008 | 1 |