Topic
TRAMP complex
About: TRAMP complex is a research topic. Over the lifetime, 259 publications have been published within this topic receiving 28287 citations. The topic is also known as: GO:0031499.
Papers published on a yearly basis
Papers
TL;DR: The exosome constitutes a highly conserved eukaryotic RNA processing complex in S. cerevisiae that is required for 3' processing of the 5.8S rRNA.
1,000 citations
TL;DR: Using a cell-free RNA decay system, it is demonstrated that the mammalian exosome is required for rapid degradation of ARE-containing RNAs but not for poly(A) shortening.
938 citations
TL;DR: It is argued that efficient mRNA turnover is required for viability and that the two major pathways of mRNA decay in yeast are identified, and the exosome's activity on mRNAs may be modulated by Ski2p, Ski3p and Ski8p.
Abstract: One major pathway of mRNA decay in yeast occurs by deadenylation-dependent decapping, which exposes the transcript to 5' to 3' exonucleolytic degradation. We show that a second general pathway of mRNA decay in yeast occurs by 3' to 5' degradation of the transcript. We also show that the SKI2, SKI3, SKI6/RRP41, SKI8 and RRP4 gene products are required for 3' to 5' decay of mRNA. The Ski6p/Rrp41p protein has homology to the Escherichia coli 3' to 5' exoribonuclease RNase PH, and both the Ski6p/Rrp41p and Rrp4p proteins are components of a multiprotein complex, termed the exosome, that contains at least three polypeptides with 3' to 5' exoribonuclease activities. These observations suggest that the exosome may be the nucleolytic activity that degrades the body of the mRNA in a 3' to 5' direction, and the exosome's activity on mRNAs may be modulated by Ski2p, Ski3p and Ski8p. Blocking both 3' to 5' and 5' to 3' decay leads to inviability, and conditional double mutants show extremely long mRNA half-lives. These observations argue that efficient mRNA turnover is required for viability and that we have identified the two major pathways of mRNA decay in yeast.
694 citations
TL;DR: Yeast mutants are used to demonstrate that these “nonstop” mRNAs are degraded by the exosome in a 3′-to-5′ direction, and the recognition of nonstop m RNAs involves the binding of Ski7p to an empty aminoacyl-(RNA-binding) site (A site) on the ribosome, thereby bringing theExosome to a mRNA with aribosome stalled near the 3′ end.
Abstract: One role of messenger RNA (mRNA) degradation is to maintain the fidelity of gene expression by degrading aberrant transcripts. Recent results show that mRNAs without translation termination codons are unstable in eukaryotic cells. We used yeast mutants to demonstrate that these “nonstop” mRNAs are degraded by the exosome in a 3′-to-5′ direction. The degradation of nonstop transcripts requires the exosome-associated protein Ski7p. Ski7p is closely related to the translation elongation factor EF1A and the translation termination factor eRF3. This suggests that the recognition of nonstop mRNAs involves the binding of Ski7p to an empty aminoacyl-(RNA-binding) site (A site) on the ribosome, thereby bringing the exosome to a mRNA with a ribosome stalled near the 3′ end. This system efficiently degrades mRNAs that are prematurely polyadenylated within the coding region and prevents their expression.
605 citations
TL;DR: To elucidate the architecture of a eukaryotic exosome, its conserved surfaces, and the structural basis for RNA decay, the X-ray structure determination for the 286 kDa nine-subunit human exosomes is reported.
555 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 2 |
| 2020 | 2 |
| 2019 | 1 |
| 2018 | 3 |
| 2017 | 15 |
| 2016 | 17 |